Construct: ORF ccsbBroad304_99992
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORFC00003.2_s304c1, BRDN0000412229
- Derived from:
- BRDN0000464768
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- Luciferase (Luciferase)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_99992
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | CONTROL | Luciferase | Luciferase | Hahn Lab Luciferase | Luciferase.1 | 100% | 100% | |
| 2 | CONTROL | LUCIFERASE | LUCIFERASE | promegaLuc | promegaLuc.1 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1719
- ORF length:
- 1650
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg 121 aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg 181 gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg 241 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa 301 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta 361 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta 421 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga 481 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt 541 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg 601 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact 661 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga 721 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt 781 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat 841 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc 901 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa 961 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc 1021 ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc 1081 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata 1141 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata 1201 ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta 1261 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc 1321 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc 1381 tgaagtctct gattaagtac aaaggctatc agGTGGCTCC CGCTGAATTG GAATCCATCT 1441 TGCTCCAACA CCCCAACATC TTCGACGCAG GTGTCGCAGG TCTTCCCGAC GATGACGCCG 1501 GTGAACTTCC CGCCGCCGTT GTTGTTTTGG AGCACGGAAA GACGATGACG GAAAAAGAGA 1561 TCGTGGATTA CGTCGCCAGT CAAGTAACAA CCGCGAAAAA GTTGCGCGGA GGAGTTGTGT 1621 TTGTGGACGA AGTACCGAAA GGTCTTACCG GAAAACTCGA CGCAAGAAAA ATCAGAGAGA 1681 TCCTCATAAA GGCCAAGAAG GGCGGAAAGA TCGCCGTGTT GCCAACTTTC TTGTACaaag 1741 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1801 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta