Construct: ORF ccsbBroadEn_00026
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009250.1_s300c1, BRDN0000390477
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADD3 (120)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 120 | ADD3 | adducin 3 | NM_001320591.2 | 100% | 100% | |
| 2 | human | 120 | ADD3 | adducin 3 | NM_001320592.2 | 100% | 100% | |
| 3 | human | 120 | ADD3 | adducin 3 | NM_001320593.2 | 100% | 100% | |
| 4 | human | 120 | ADD3 | adducin 3 | NM_016824.5 | 100% | 100% | |
| 5 | human | 120 | ADD3 | adducin 3 | XM_024447794.1 | 100% | 100% | |
| 6 | human | 120 | ADD3 | adducin 3 | XM_024447795.1 | 100% | 100% | |
| 7 | human | 120 | ADD3 | adducin 3 | XM_024447796.1 | 100% | 100% | |
| 8 | human | 120 | ADD3 | adducin 3 | XM_024447797.1 | 100% | 100% | |
| 9 | human | 120 | ADD3 | adducin 3 | XM_024447798.1 | 100% | 100% | |
| 10 | human | 120 | ADD3 | adducin 3 | XM_024447799.1 | 100% | 100% | |
| 11 | human | 120 | ADD3 | adducin 3 | XM_024447800.1 | 100% | 100% | |
| 12 | human | 120 | ADD3 | adducin 3 | XM_024447801.1 | 100% | 100% | |
| 13 | human | 120 | ADD3 | adducin 3 | XM_024447802.1 | 100% | 100% | |
| 14 | human | 120 | ADD3 | adducin 3 | NM_001121.4 | 95.4% | 95.4% | 1726_1727ins96 |
| 15 | human | 120 | ADD3 | adducin 3 | NM_019903.5 | 95.4% | 95.4% | 1726_1727ins96 |
| 16 | human | 120 | ADD3 | adducin 3 | XM_024447803.1 | 95.4% | 95.4% | 1726_1727ins96 |
| 17 | human | 120 | ADD3 | adducin 3 | XM_024447804.1 | 95.4% | 95.4% | 1726_1727ins96 |
| 18 | human | 120 | ADD3 | adducin 3 | XM_024447805.1 | 95.4% | 95.4% | 1726_1727ins96 |
| 19 | human | 120 | ADD3 | adducin 3 | XM_024447806.1 | 95.4% | 95.4% | 1726_1727ins96 |
| 20 | human | 120 | ADD3 | adducin 3 | XM_024447807.1 | 95.4% | 95.4% | 1726_1727ins96 |
| 21 | human | 120 | ADD3 | adducin 3 | NM_001320594.2 | 88.9% | 88.9% | 0_1ins234 |
| 22 | human | 120 | ADD3 | adducin 3 | XM_024447808.1 | 88.9% | 88.9% | 0_1ins234 |
| 23 | human | 120 | ADD3 | adducin 3 | XM_024447809.1 | 84.4% | 84.4% | 0_1ins234;1492_1493ins96 |
| 24 | human | 120 | ADD3 | adducin 3 | XM_024447810.1 | 84.4% | 84.4% | 0_1ins234;1492_1493ins96 |
| 25 | human | 120 | ADD3 | adducin 3 | XM_024447811.1 | 65.7% | 65.7% | 0_1ins726 |
| 26 | human | 120 | ADD3 | adducin 3 | XM_024447812.1 | 65.7% | 65.7% | 0_1ins726 |
| 27 | human | 120 | ADD3 | adducin 3 | XM_024447813.1 | 65.7% | 65.7% | 0_1ins726 |
| 28 | human | 120 | ADD3 | adducin 3 | XM_024447814.1 | 61.1% | 61.1% | 0_1ins726;1000_1001ins96 |
| 29 | mouse | 27360 | Add3 | adducin 3 (gamma) | NM_001164099.2 | 87% | 92.2% | (many diffs) |
| 30 | mouse | 27360 | Add3 | adducin 3 (gamma) | NM_001277100.1 | 87% | 92.2% | (many diffs) |
| 31 | mouse | 27360 | Add3 | adducin 3 (gamma) | NM_013758.4 | 87% | 92.2% | (many diffs) |
| 32 | mouse | 27360 | Add3 | adducin 3 (gamma) | NM_001164100.2 | 82.9% | 88.3% | (many diffs) |
| 33 | mouse | 27360 | Add3 | adducin 3 (gamma) | NM_001164101.2 | 82.9% | 88.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2184
- ORF length:
- 2118
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAG CTCAGATGCC AGCCAAGGCG TGATTACCAC TCCTCCTCCT CCCAGCATGC 121 CTCACAAAGA GAGATATTTT GACCGCATCA ATGAAAATGA CCCAGAATAC ATTAGGGAGA 181 GGAACATGTC TCCTGATCTA CGACAAGACT TCAACATGAT GGAGCAGAGG AAACGAGTTA 241 CTCAGATCCT GCAAAGTCCT GCCTTTCGGG AAGACTTGGA ATGCCTTATT CAAGAACAGA 301 TGAAGAAAGG CCACAACCCA ACTGGATTAC TAGCATTACA GCAGATTGCA GATTACATCA 361 TGGCCAATTC TTTCTCGGGT TTTTCTTCAC CTCCTCTCAG TCTTGGCATG GTCACACCTA 421 TCAATGACCT TCCTGGTGCA GATACATCCT CATATGTGAA GGGAGAAAAA CTTACTCGCT 481 GTAAACTTGC CAGCCTGTAC AGACTTGTAG ACTTGTTTGG ATGGGCACAC CTGGCAAATA 541 CCTATATCTC AGTAAGAATA AGTAAGGAGC AAGACCACAT TATAATAATT CCCAGAGGCC 601 TATCTTTTTC TGAAGCTACA GCCTCCAATT TGGTGAAAGT CAATATAATA GGAGAAGTGG 661 TTGACCAGGG AAGTACCAAT TTGAAAATTG ACCATACAGG ATTCAGTCCC CATGCTGCAA 721 TCTATTCAAC ACGTCCTGAT GTTAAGTGTG TGATACACAT CCATACCCTT GCAACAGCAG 781 CTGTATCCTC CATGAAATGT GGGATCCTTC CAATTTCTCA AGAGTCTCTT CTTCTGGGAG 841 ATGTTGCCTA TTATGACTAC CAAGGGTCAC TTGAAGAACA GGAGGAGAGA ATTCAACTGC 901 AGAAGGTTCT GGGACCAAGT TGTAAGGTGC TGGTACTCAG GAATCATGGT GTGGTTGCAC 961 TTGGAGAAAC ATTAGAGGAG GCTTTTCATT ATATTTTTAA TGTGCAACTA GCCTGTGAGA 1021 TTCAGGTGCA GGCCCTAGCA GGTGCAGGTG GAGTAGACAA TCTCCATGTA CTGGACTTTC 1081 AGAAGTATAA AGCTTTCACT TACACTGTAG CAGCGTCTGG TGGAGGAGGT GTGAATATGG 1141 GTTCCCATCA AAAATGGAAG GTTGGCGAAA TTGAGTTTGA AGGGCTTATG AGGACTCTGG 1201 ACAACTTGGG GTATAGAACA GGCTATGCTT ACAGGCATCC TCTCATTCGA GAGAAGCCTA 1261 GGCACAAGAG TGATGTGGAA ATCCCAGCAA CTGTGACTGC TTTTTCCTTT GAAGACGATA 1321 CAGTGCCACT CTCTCCTCTC AAATACATGG CACAGAGGCA ACAGCGTGAA AAAACAAGAT 1381 GGCTGAACTC ACCAAATACT TACATGAAAG TGAATGTGCC TGAGGAGTCT CGGAACGGAG 1441 AAACCAGTCC CCGAACCAAA ATCACGTGGA TGAAAGCAGA AGACTCATCT AAAGTTAGTG 1501 GTGGAACACC TATCAAAATT GAAGATCCAA ATCAGTTTGT TCCTTTAAAC ACAAACCCGA 1561 ATGAGGTACT AGAAAAGAGA AATAAGATTC GGGAACAAAA TCGATATGAC TTGAAAACAG 1621 CAGGACCACA ATCTCAGTTG CTTGCTGGAA TTGTTGTGGA TAAGCCACCT TCTACTATGC 1681 AATTTGAAGA TGATGATCAT GGCCCACCAG CTCCTCCTAA CCCATTTAGT CATCTCACAG 1741 AAGGAGAACT TGAAGAGTAT AAGAGGACAA TCGAACGTAA ACAACAAGGC CTAGAAGATG 1801 CTGAGCAGGA ATTACTCTCA GATGACGCTT CATCTGTTTC ACAAATTCAG TCTCAAACTC 1861 AGTCACCGCA AAATGTCCCT GAAAAATTAG AAGAAAACCA TGAGCTGTTT TCCAAGAGCT 1921 TCATCTCCAT GGAAGTGCCT GTCATGGTAG TAAATGGCAA GGATGATATG CATGATGTTG 1981 AAGATGAGCT TGCTAAGCGA GTGAGTAGGT TAAGCACAAG TACAACCATA GAAAACATCG 2041 AGATTACTAT TAAGTCTCCA GAGAAAATCG AAGAAGTCCT GTCACCTGAA GGCTCCCCTT 2101 CAAAATCGCC ATCCAAGAAA AAGAAGAAAT TCCGCACTCC TTCTTTTCTG AAAAAGAACA 2161 AAAAAAAGGA GAAAGTTGAG GCCTACCCAA CTTTCTTGTA CAAAGTtggc attataagaa 2221 agcattgctt atcaatttgt tgcaacgaac