Construct: ORF ccsbBroadEn_00055

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014476.1_s300c1, BRDN0000392423
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AKR1B1 (231)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 231 AKR1B1 aldo-keto reductase family ... NM_001628.4 100% 100%
2 human 231 AKR1B1 aldo-keto reductase family ... NM_001346142.1 54.4% 54.4% 0_1ins432
3 human 231 AKR1B1 aldo-keto reductase family ... XM_005250234.3 54.4% 54.4% 0_1ins432
4 human 231 AKR1B1 aldo-keto reductase family ... XM_024446695.1 54.4% 54.4% 0_1ins432
5 human 231 AKR1B1 aldo-keto reductase family ... NR_144376.1 45.6% 1_130del;869_1466del;1677_2078del
6 mouse 11677 Akr1b3 aldo-keto reductase family ... NM_009658.3 85.7% 85.4% (many diffs)
7 mouse 626009 Gm6644 predicted gene 6644; Akr1b3... NR_037965.1 59.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1014
ORF length:
948
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC AAGCCGTCTC CTGCTCAACA ACGGCGCCAA GATGCCCATC CTGGGGTTGG
121GTACCTGGAA GTCCCCTCCA GGGCAGGTGA CTGAGGCCGT GAAGGTGGCC ATTGACGTCG
181GGTACCGCCA CATCGACTGT GCCCATGTGT ACCAGAATGA GAATGAGGTG GGGGTGGCCA
241TTCAGGAGAA GCTCAGGGAG CAGGTGGTGA AGCGTGAGGA GCTCTTCATC GTCAGCAAGC
301TGTGGTGCAC GTACCATGAG AAGGGCCTGG TGAAAGGAGC CTGCCAGAAG ACACTCAGCG
361ACCTGAAGCT GGACTACCTG GACCTCTACC TTATTCACTG GCCGACTGGC TTTAAGCCTG
421GGAAGGAATT TTTCCCATTG GATGAGTCGG GCAATGTGGT TCCCAGTGAC ACCAACATTC
481TGGACACGTG GGCGGCCATG GAAGAGCTGG TGGATGAAGG GCTGGTGAAA GCTATTGGCA
541TCTCCAACTT CAACCATCTC CAGGTGGAGA TGATCTTAAA CAAACCTGGC TTGAAGTATA
601AGCCTGCAGT TAACCAGATT GAGTGCCACC CATATCTCAC TCAGGAGAAG TTAATCCAGT
661ACTGCCAGTC CAAAGGCATC GTGGTGACCG CCTACAGCCC CCTCGGCTCT CCTGACAGGC
721CCTGGGCCAA GCCCGAGGAC CCTTCTCTCC TGGAGGATCC CAGGATCAAG GCGATCGCAG
781CCAAGCACAA TAAAACTACA GCCCAGGTCC TGATCCGGTT CCCCATGCAG AGGAACTTGG
841TGGTGATCCC CAAGTCTGTG ACACCAGAAC GCATTGCTGA GAACTTTAAG GTCTTTGACT
901TTGAACTGAG CAGCCAGGAT ATGACCACCT TACTCAGCTA CAACAGGAAC TGGAGGGTCT
961GTGCCTTGTT GAGCTGTACC TCCCACAAGG ATTACCCCTT CCATGAAGAG TTTTGCCCAA
1021CTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)