Construct: ORF ccsbBroadEn_00219

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001343.1_s300c1, BRDN0000395710
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CARS1 (833)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 833 CARS1 cysteinyl-tRNA synthetase 1 NM_001751.6 100% 100%
2 human 833 CARS1 cysteinyl-tRNA synthetase 1 NM_139273.4 96.3% 94.2% 2111_2118delATGTAAGC;2178_2179ins74
3 human 833 CARS1 cysteinyl-tRNA synthetase 1 XM_024448703.1 95.4% 95.4% 1274_1381del
4 human 833 CARS1 cysteinyl-tRNA synthetase 1 XM_006718341.3 94.5% 94.5% 0_1ins77;38_84del
5 human 833 CARS1 cysteinyl-tRNA synthetase 1 XM_017018392.2 94.2% 92% (many diffs)
6 human 833 CARS1 cysteinyl-tRNA synthetase 1 XM_017018389.1 91% 88.9% (many diffs)
7 human 833 CARS1 cysteinyl-tRNA synthetase 1 XM_017018390.1 91% 88.9% (many diffs)
8 human 833 CARS1 cysteinyl-tRNA synthetase 1 XM_024448704.1 90.3% 90.2% 0_1ins77;38_84del;1244_1351del
9 human 833 CARS1 cysteinyl-tRNA synthetase 1 NM_001014437.3 89.8% 89.1% (many diffs)
10 human 833 CARS1 cysteinyl-tRNA synthetase 1 XM_017018391.1 87.7% 87.7% 0_1ins276
11 human 833 CARS1 cysteinyl-tRNA synthetase 1 NM_001194997.2 86.5% 84% (many diffs)
12 human 833 CARS1 cysteinyl-tRNA synthetase 1 XM_024448701.1 86% 85.4% (many diffs)
13 human 833 CARS1 cysteinyl-tRNA synthetase 1 XR_001747995.2 86% 1_56del;2085_2205del;2422_2609del
14 human 833 CARS1 cysteinyl-tRNA synthetase 1 XR_001747996.2 85.7% (many diffs)
15 human 833 CARS1 cysteinyl-tRNA synthetase 1 XM_024448705.1 83.6% 83.6% 0_1ins276;998_1105del
16 human 833 CARS1 cysteinyl-tRNA synthetase 1 XM_024448702.1 82.9% 80.5% (many diffs)
17 human 833 CARS1 cysteinyl-tRNA synthetase 1 XR_930914.2 80.9% (many diffs)
18 human 833 CARS1 cysteinyl-tRNA synthetase 1 XR_001747998.1 80.7% (many diffs)
19 human 833 CARS1 cysteinyl-tRNA synthetase 1 NR_036542.2 80.4% (many diffs)
20 human 833 CARS1 cysteinyl-tRNA synthetase 1 XR_930913.1 78.1% (many diffs)
21 human 833 CARS1 cysteinyl-tRNA synthetase 1 XR_001747997.1 77% (many diffs)
22 human 833 CARS1 cysteinyl-tRNA synthetase 1 XR_002957203.1 75.4% (many diffs)
23 human 833 CARS1 cysteinyl-tRNA synthetase 1 XR_002957204.1 75.2% (many diffs)
24 mouse 27267 Cars cysteinyl-tRNA synthetase NM_001252593.1 85.7% 90.2% (many diffs)
25 mouse 27267 Cars cysteinyl-tRNA synthetase NM_013742.5 76.9% 80.9% (many diffs)
26 mouse 27267 Cars cysteinyl-tRNA synthetase XM_011242000.1 75.2% 79.1% (many diffs)
27 mouse 27267 Cars cysteinyl-tRNA synthetase XM_011242001.1 75.2% 79.1% (many diffs)
28 mouse 27267 Cars cysteinyl-tRNA synthetase XM_006508627.2 74.9% 78.9% (many diffs)
29 mouse 27267 Cars cysteinyl-tRNA synthetase XR_378611.2 67.9% (many diffs)
30 mouse 27267 Cars cysteinyl-tRNA synthetase XM_017322295.1 62.6% 65.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2310
ORF length:
2244
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC AGATTCCTCC GGGCAGCAGG GCAAAGGCCG GCGTGTGCAG CCCCAGTGGT
121CCCCTCCTGC TGGGACCCAG CCATGCAGAC TCCACCTTTA CAACAGCCTC ACCAGGAACA
181AGGAAGTGTT CATACCTCAA GATGGGAAAA AGGTGACGTG GTATTGCTGT GGGCCAACCG
241TCTATGACGC ATCTCACATG GGGCACGCCA GGTCCTACAT CTCTTTTGAT ATCTTGAGAA
301GAGTGTTGAA GGATTACTTC AAATTTGATG TCTTTTATTG CATGAACATT ACGGATATTG
361ATGACAAGAT CATCAAGAGG GCCCGGCAGA ACCACCTGTT CGAGCAGTAT CGGGAGAAGA
421GGCCTGAAGC GGCACAGCTC TTGGAGGATG TTCAGGCCGC CCTGAAGCCA TTTTCAGTAA
481AATTAAATGA GACCACGGAT CCCGATAAAA AGCAGATGCT CGAACGGATT CAGCACGCAG
541TGCAGCTTGC CACAGAGCCA CTTGAGAAAG CTGTGCAGTC CAGACTCACG GGAGAGGAAG
601TCAACAGCTG TGTGGAGGTG TTGCTGGAAG AAGCCAAGGA TTTGCTCTCT GACTGGCTGG
661ATTCTACACT TGGCTGTGAT GTCACTGACA ATTCCATCTT CTCCAAGCTG CCCAAGTTCT
721GGGAGGGGGA CTTCCACAGA GACATGGAAG CTCTGAATGT TCTCCCTCCA GATGTCTTAA
781CCCGGGTTAG TGAGTATGTG CCAGAAATTG TGAACTTTGT CCAGAAGATT GTGGACAACG
841GTTACGGCTA TGTCTCCAAT GGGTCTGTCT ACTTTGATAC AGCGAAGTTT GCTTCTAGCG
901AGAAGCACTC CTATGGGAAG CTGGTGCCTG AGGCCGTTGG AGATCAGAAA GCCCTTCAAG
961AAGGGGAAGG TGACCTGAGC ATCTCTGCAG ACCGCCTGAG TGAGAAGCGC TCTCCCAACG
1021ACTTTGCCTT ATGGAAGGCC TCTAAGCCCG GAGAACCGTC CTGGCCGTGC CCTTGGGGAA
1081AGGGTCGTCC GGGCTGGCAT ATCGAGTGCT CGGCCATGGC AGGCACCCTC CTAGGGGCTT
1141CGATGGACAT TCACGGAGGT GGGTTCGACC TCCGGTTCCC CCACCATGAC AATGAGCTGG
1201CACAGTCGGA GGCCTACTTT GAAAACGACT GCTGGGTCAG GTACTTCCTG CACACAGGCC
1261ACCTGACCAT TGCAGGCTGC AAAATGTCAA AGTCACTAAA AAACTTCATC ACCATTAAAG
1321ATGCCTTGAA AAAGCACTCA GCACGGCAGT TGCGGCTGGC CTTCCTCATG CACTCGTGGA
1381AGGACACCCT GGACTACTCC AGCAACACCA TGGAGTCAGC GCTTCAATAT GAGAAGTTCT
1441TGAATGAGTT TTTCTTAAAT GTGAAAGATA TCCTTCGCGC TCCTGTTGAC ATCACTGGTC
1501AGTTTGAGAA GTGGGGAGAA GAAGAAGCAG AACTGAATAA GAACTTTTAT GACAAGAAGA
1561CAGCAATTCA CAAAGCCCTC TGTGACAATG TTGACACCCG CACCGTCATG GAAGAGATGC
1621GGGCCTTGGT CAGTCAGTGC AACCTCTATA TGGCAGCCCG GAAAGCCGTG AGGAAGAGGC
1681CCAACCAGGC TCTGCTGGAG AACATCGCCC TGTACCTCAC CCATATGCTG AAGATCTTTG
1741GGGCCGTAGA AGAGGACAGC TCCCTGGGAT TCCCGGTCGG AGGGCCTGGA ACCAGCCTCA
1801GTCTCGAGGC CACAGTCATG CCCTACCTTC AGGTGTTATC AGAATTCCGA GAAGGAGTGC
1861GGAAGATTGC CCGAGAGCAA AAAGTCCCTG AGATTCTGCA GCTCAGCGAT GCCCTGCGGG
1921ACAACATCCT GCCCGAGCTT GGGGTGCGGT TTGAAGACCA CGAAGGACTG CCCACAGTGG
1981TGAAACTGGT AGACAGAAAC ACCTTATTAA AAGAGAGAGA AGAAAAGAGA CGGGTTGAAG
2041AGGAGAAGAG GAAGAAGAAA GAGGAGGCGG CCCGGAGGAA ACAGGAACAA GAAGCAGCAA
2101AGCTGGCCAA GATGAAGATT CCCCCCAGTG AGATGTTCTT GTCAGAAACC GACAAATACT
2161CCAAGTTTGA TGAAAATGGT CTGCCCACAC ATGACATGGA GGGCAAAGAG CTCAGCAAAG
2221GGCAAGCCAA GAAGCTGAAG AAGCTCTTCG AGGCTCAGGA GAAGCTCTAC AAGGAATATC
2281TGCAGATGGC CCAGAATGGA AGCTTCCAGT GCCCAACTTT CTTGTACAAA GTtggcatta
2341taagaaagca ttgcttatca atttgttgca acgaac

Download FASTA (ORF) (Full)