Construct: ORF ccsbBroadEn_00238
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011374.1_s300c1, BRDN0000397568
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCNE1 (898)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 898 | CCNE1 | cyclin E1 | NM_001238.4 | 100% | 100% | |
| 2 | human | 898 | CCNE1 | cyclin E1 | XM_011527440.2 | 99.2% | 99.2% | 111_112insTTTTTGCAG |
| 3 | human | 898 | CCNE1 | cyclin E1 | NM_001322262.2 | 96.3% | 96.3% | 0_1ins45 |
| 4 | human | 898 | CCNE1 | cyclin E1 | NM_001322259.2 | 89% | 89% | 703_704ins135 |
| 5 | human | 898 | CCNE1 | cyclin E1 | NM_001322261.2 | 88% | 88% | 459_460ins147 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1296
- ORF length:
- 1230
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCC GAGGGAGCGC AGGGAGCGGG ATGCGAAGGA GCGGGACACC ATGAAGGAGG 121 ACGGCGGCGC GGAGTTCTCG GCTCGCTCCA GGAAGAGGAA GGCAAACGTG ACCGTTTTTT 181 TGCAGGATCC AGATGAAGAA ATGGCCAAAA TCGACAGGAC GGCGAGGGAC CAGTGTGGGA 241 GCCAGCCTTG GGACAATAAT GCAGTCTGTG CAGACCCCTG CTCCCTGATC CCCACACCTG 301 ACAAAGAAGA TGATGACCGG GTTTACCCAA ACTCAACGTG CAAGCCTCGG ATTATTGCAC 361 CATCCAGAGG CTCCCCGCTG CCTGTACTGA GCTGGGCAAA TAGAGAGGAA GTCTGGAAAA 421 TCATGTTAAA CAAGGAAAAG ACATACTTAA GGGATCAGCA CTTTCTTGAG CAACACCCTC 481 TTCTGCAGCC AAAAATGCGA GCAATTCTTC TGGATTGGTT AATGGAGGTG TGTGAAGTCT 541 ATAAACTTCA CAGGGAGACC TTTTACTTGG CACAAGATTT CTTTGACCGG TATATGGCGA 601 CACAAGAAAA TGTTGTAAAA ACTCTTTTAC AGCTTATTGG GATTTCATCT TTATTTATTG 661 CAGCCAAACT TGAGGAAATC TATCCTCCAA AGTTGCACCA GTTTGCGTAT GTGACAGATG 721 GAGCTTGTTC AGGAGATGAA ATTCTCACCA TGGAATTAAT GATTATGAAG GCCCTTAAGT 781 GGCGTTTAAG TCCCCTGACT ATTGTGTCCT GGCTGAATGT ATACATGCAG GTTGCATATC 841 TAAATGACTT ACATGAAGTG CTACTGCCGC AGTATCCCCA GCAAATCTTT ATACAGATTG 901 CAGAGCTGTT GGATCTCTGT GTCCTGGATG TTGACTGCCT TGAATTTCCT TATGGTATAC 961 TTGCTGCTTC GGCCTTGTAT CATTTCTCGT CATCTGAATT GATGCAAAAG GTTTCAGGGT 1021 ATCAGTGGTG CGACATAGAG AACTGTGTCA AGTGGATGGT TCCATTTGCC ATGGTTATAA 1081 GGGAGACGGG GAGCTCAAAA CTGAAGCACT TCAGGGGCGT CGCTGATGAA GATGCACACA 1141 ACATACAGAC CCACAGAGAC AGCTTGGATT TGCTGGACAA AGCCCGAGCA AAGAAAGCCA 1201 TGTTGTCTGA ACAAAATAGG GCTTCTCCTC TCCCCAGTGG GCTCCTCACC CCGCCACAGA 1261 GCGGTAAGAA GCAGAGCAGC GGGCCGGAAA TGGCGTGCCC AACTTTCTTG TACAAAGTtg 1321 gcattataag aaagcattgc ttatcaattt gttgcaacga ac