Construct: ORF ccsbBroadEn_00470

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003957.1_s300c1, BRDN0000386580
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DVL2 (1856)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1856 DVL2 dishevelled segment polarit... NM_004422.3 100% 100%
2 human 1856 DVL2 dishevelled segment polarit... XM_005256502.2 99.4% 99.3% 508_509insGTGAGCATGGCG
3 human 1856 DVL2 dishevelled segment polarit... XR_001752443.2 78.9% (many diffs)
4 human 1856 DVL2 dishevelled segment polarit... XR_001752442.2 71.6% 1_282del;1826_1891del;2557_3083del
5 human 1856 DVL2 dishevelled segment polarit... XR_001752441.2 63.6% (many diffs)
6 mouse 13543 Dvl2 dishevelled segment polarit... NM_007888.3 88.1% 96.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2274
ORF length:
2208
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GGGTAGCAGC ACTGGGGGCG GTGGGGTTGG GGAGACGAAG GTGATTTACC
121ACCTGGATGA GGAAGAGACT CCCTACCTGG TGAAGATCCC TGTCCCCGCC GAGCGCATCA
181CCCTCGGCGA TTTCAAGAGC GTCCTGCAGC GGCCCGCGGG CGCCAAGTAC TTTTTCAAGT
241CTATGGATCA GGATTTCGGG GTGGTGAAGG AAGAAATTTC AGATGACAAC GCCCGCCTCC
301CCTGCTTCAA CGGAAGGGTG GTATCCTGGC TGGTGTCCTC AGATAATCCC CAACCCGAGA
361TGGCCCCTCC AGTCCATGAG CCTCGGGCAG AACTGGCGCC TCCAGCCCCA CCTTTACCTC
421CTTTGCCACC CGAGAGGACC AGCGGCATTG GGGACTCAAG GCCTCCATCC TTCCACCCTA
481ATGTGTCCAG CAGCCATGAG AATCTGGAGC CTGAGACAGA AACCGAGTCA GTAGTGTCAC
541TGAGGCGGGA GCGGCCTCGC AGGAGAGACA GCAGTGAGCA TGGCGCTGGG GGCCACAGGA
601CTGGTGGCCC CTCAAGGCTG GAGCGCCACC TGGCCGGATA CGAGAGCTCC TCTACCCTCA
661TGACCAGCGA GCTGGAGAGT ACCAGCCTGG GGGACTCGGA CGAGGAGGAC ACCATGAGCA
721GGTTCAGCAG CTCCACGGAG CAGAGCAGTG CCTCCCGCCT CCTTAAGCGC CACCGGCGGC
781GAAGGAAGCA GAGGCCACCC CGCCTGGAGA GGACGTCATC CTTCAGCAGC GTCACAGATT
841CCACAATGTC TCTCAATATC ATCACAGTCA CGCTAAACAT GGAGAAGTAC AACTTCCTGG
901GTATCTCCAT TGTTGGCCAG AGCAATGAGC GGGGAGACGG AGGCATCTAC ATTGGCTCCA
961TCATGAAGGG TGGGGCTGTG GCGGCCGACG GGCGCATTGA GCCAGGGGAC ATGCTTTTGC
1021AGGTGAATGA CATGAACTTT GAGAACATGA GCAACGATGA CGCTGTGCGG GTGCTGAGGG
1081ACATTGTGCA CAAGCCTGGC CCCATTGTGC TGACTGTGGC CAAGTGCTGG GATCCCTCTC
1141CTCAGGCCTA TTTCACTCTC CCCCGAAATG AGCCCATCCA GCCAATTGAC CCTGCTGCCT
1201GGGTGTCCCA TTCCGCGGCT CTGACTGGCA CCTTCCCAGC CTATCCAGGT TCCTCCTCCA
1261TGAGCACCAT TACATCTGGA TCGTCTTTGC CTGATGGCTG TGAAGGCCGG GGTCTCTCCG
1321TCCATACGGA CATGGCATCG GTGACCAAGG CCATGGCAGC TCCAGAGTCT GGACTGGAAG
1381TCCGGGACCG CATGTGGCTC AAGATCACCA TCCCTAATGC CTTTCTGGGC TCGGATGTGG
1441TTGACTGGCT CTACCATCAC GTGGAGGGCT TTCCTGAGCG GCGGGAGGCC CGCAAGTATG
1501CCAGCGGGCT GCTCAAAGCA GGCCTGATCC GACACACCGT CAACAAGATC ACCTTCTCTG
1561AGCAGTGCTA TTACGTCTTC GGAGACCTCA GTGGTGGCTG TGAGAGCTAC CTAGTCAACC
1621TGTCTCTCAA TGACAACGAT GGCTCCAGTG GGGCTTCAGA CCAGGATACC CTGGCTCCTC
1681TGCCTGGGGC CACCCCCTGG CCCCTGCTGC CCACTTTCTC CTACCAATAC CCTGCCCCAC
1741ACCCCTACAG CCCGCAGCCT CCACCCTACC ATGAGCTTTC ATCTTACACC TATGGTGGGG
1801GCAGTGCCAG CAGCCAGCAT AGTGAGGGCA GCCGGAGCAG TGGGTCGACA CGGAGTGATG
1861GGGGGGCAGG GCGCACGGGG AGGCCCGAGG AGCGGGCCCC CGAGTCCAAG TCCGGCAGTG
1921GCAGTGAGTC TGAGCCCTCC AGCCGAGGGG GCAGCCTTCG GCGGGGTGGG GAAGCAAGTG
1981GGACTAGCGA TGGGGGCCCT CCTCCATCCA GAGGCTCAAC TGGGGGTGCC CCTAATCTCC
2041GAGCCCACCC AGGGCTCCAT CCCTATGGAC CGCCCCCTGG CATGGCCCTC CCCTACAACC
2101CCATGATGGT GGTCATGATG CCCCCACCTC CACCTCCAGT CCCTCCAGCA GTGCAGCCTC
2161CGGGGGCCCC TCCAGTCAGA GACCTGGGCT CTGTGCCCCC AGAACTGACA GCCAGCCGCC
2221AAAGCTTCCA CATGGCCATG GGCAATCCCA GCGAGTTCTT TGTGGATGTT ATGTACCCAA
2281CTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)