Construct: ORF ccsbBroadEn_00523

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016535.1_s300c1, BRDN0000395709
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EXT1 (2131)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2131 EXT1 exostosin glycosyltransfera... NM_000127.2 100% 100%
2 mouse 14042 Ext1 exostoses (multiple) 1 NM_010162.2 95.8% 99.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2304
ORF length:
2238
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCA GGCCAAAAAA CGCTATTTCA TCCTGCTCTC AGCTGGCTCT TGTCTCGCCC
121TTTTGTTTTA TTTCGGAGGC TTGCAGTTTA GGGCATCGAG GAGCCACAGC CGGAGAGAAG
181AACACAGCGG TAGGAATGGC TTGCACCACC CCAGTCCGGA TCATTTCTGG CCCCGCTTCC
241CGGACGCTCT GCGCCCCTTC GTTCCTTGGG ATCAATTGGA AAACGAGGAT TCCAGCGTGC
301ACATTTCCCC CCGGCAGAAG CGAGATGCCA ACTCCAGCAT CTACAAAGGC AAGAAGTGCC
361GCATGGAGTC CTGCTTCGAT TTCACCCTTT GCAAGAAAAA CGGCTTCAAA GTCTACGTAT
421ACCCACAGCA AAAAGGGGAG AAAATCGCCG AAAGTTACCA AAACATTCTA GCGGCCATCG
481AGGGCTCCAG GTTCTACACC TCGGACCCCA GCCAGGCGTG CCTCTTTGTC CTGAGTCTGG
541ATACTTTAGA CAGAGACCAG TTGTCACCTC AGTATGTGCA CAATTTGAGA TCCAAAGTGC
601AGAGTCTCCA CTTGTGGAAC AATGGTAGGA ATCATTTAAT TTTTAATTTA TATTCCGGCA
661CTTGGCCTGA CTACACCGAG GACGTGGGGT TTGACATCGG CCAGGCGATG CTGGCCAAAG
721CCAGCATCAG TACTGAAAAC TTCCGACCCA ACTTTGATGT TTCTATTCCC CTCTTTTCTA
781AGGATCATCC CAGGACAGGA GGGGAGAGGG GGTTTTTGAA GTTCAACACC ATCCCTCCTC
841TCAGGAAGTA CATGCTGGTA TTCAAGGGGA AGAGGTACCT GACAGGGATA GGATCAGACA
901CCAGGAATGC CTTATATCAC GTCCATAACG GGGAGGACGT TGTGCTCCTC ACCACCTGCA
961AGCATGGCAA AGACTGGCAA AAGCACAAGG ATTCTCGCTG TGACAGAGAC AACACCGAGT
1021ATGAGAAGTA TGATTATCGG GAAATGCTGC ACAATGCCAC TTTCTGTCTG GTTCCTCGTG
1081GTCGCAGGCT TGGGTCCTTC AGATTCCTGG AGGCTTTGCA GGCTGCCTGC GTCCCTGTGA
1141TGCTCAGCAA TGGATGGGAG TTGCCATTCT CTGAAGTGAT TAATTGGAAC CAAGCTGCCG
1201TCATAGGCGA TGAGAGATTG TTATTACAGA TTCCTTCTAC AATCAGGTCT ATTCATCAGG
1261ATAAAATCCT AGCACTTAGA CAGCAGACAC AATTCTTGTG GGAGGCTTAT TTTTCTTCAG
1321TTGAGAAGAT TGTATTAACT ACACTAGAGA TTATTCAGGA CAGAATATTC AAGCACATAT
1381CACGTAACAG TTTAATATGG AACAAACATC CTGGAGGATT GTTCGTACTA CCACAGTATT
1441CATCTTATCT GGGAGATTTT CCTTACTACT ATGCTAATTT AGGTTTAAAG CCCCCCTCCA
1501AATTCACTGC AGTCATCCAT GCGGTGACCC CCCTGGTCTC TCAGTCCCAG CCAGTGTTGA
1561AGCTTCTCGT GGCTGCAGCC AAGTCCCAGT ACTGTGCCCA GATCATAGTT CTATGGAATT
1621GTGACAAGCC CCTACCAGCC AAACACCGCT GGCCTGCCAC TGCTGTGCCT GTCGTCGTCA
1681TTGAAGGAGA GAGCAAGGTT ATGAGCAGCC GTTTTCTGCC CTACGACAAC ATCATCACAG
1741ACGCCGTGCT CAGCCTTGAC GAGGACACGG TGCTTTCAAC AACAGAGGTG GATTTCGCCT
1801TCACAGTGTG GCAGAGCTTC CCTGAGAGGA TTGTGGGGTA CCCCGCGCGC AGCCACTTCT
1861GGGATAACTC TAAGGAGCGG TGGGGATACA CATCAAAGTG GACGAACGAC TACTCCATGG
1921TGTTGACAGG AGCTGCTATT TACCACAAAT ATTATCACTA CCTATACTCC CATTACCTGC
1981CAGCCAGCCT GAAGAACATG GTGGACCAAT TGGCCAATTG TGAGGACATT CTCATGAACT
2041TCCTGGTGTC TGCTGTGACA AAATTGCCTC CAATCAAAGT GACCCAGAAG AAGCAGTATA
2101AGGAGACAAT GATGGGACAG ACTTCTCGGG CTTCCCGTTG GGCTGACCCT GACCACTTTG
2161CCCAGCGACA GAGCTGCATG AATACGTTTG CCAGCTGGTT TGGCTACATG CCGCTGATCC
2221ACTCTCAGAT GAGGCTCGAC CCCGTCCTCT TTAAAGACCA GGTCTCTATT TTGAGGAAGA
2281AATACCGAGA CATTGAGCGA CTTTGCCCAA CTTTCTTGTA CAAAGTtggc attataagaa
2341agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)