Construct: ORF ccsbBroadEn_00526

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015219.1_s300c1, BRDN0000386598
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EZH2 (2146)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2146 EZH2 enhancer of zeste 2 polycom... NM_004456.5 100% 100%
2 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515884.2 100% 100%
3 human 2146 EZH2 enhancer of zeste 2 polycom... NM_001203247.2 99.3% 99.2% 892_893insGTAAGTGCAATTATT
4 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515883.2 98.9% 98.9% 1_24del
5 human 2146 EZH2 enhancer of zeste 2 polycom... XM_017011817.2 98.9% 98.9% 1_24del
6 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515887.3 98.8% 98.8% 219_220ins27
7 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515888.2 98.8% 98.8% 219_220ins27
8 human 2146 EZH2 enhancer of zeste 2 polycom... XM_005249962.4 98.2% 98.1% 1_24del;916_917insGTAAGTGCAATTATT
9 human 2146 EZH2 enhancer of zeste 2 polycom... NM_001203248.2 98.1% 98% 219_220ins27;865_866insGTAAGTGCAATTATT
10 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515885.2 97.7% 97.7% 1_24del;243_244ins27
11 human 2146 EZH2 enhancer of zeste 2 polycom... XM_005249963.4 97.1% 96.9% 1_24del;243_244ins27;889_890insGTAAGTGCAATTATT
12 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515894.2 94.8% 94.8% 245_246ins117
13 human 2146 EZH2 enhancer of zeste 2 polycom... NM_152998.3 94.1% 94% 245_246ins117;775_776insGTAAGTGCAATTATT
14 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515890.2 93.8% 93.8% 1_24del;269_270ins117
15 human 2146 EZH2 enhancer of zeste 2 polycom... XM_017011820.2 93.6% 93.6% 219_220ins144
16 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515892.2 93.4% 93.2% 1_24del;1570_1571ins126
17 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515893.2 93.1% 93% 1_24del;269_270ins117;799_800insGTAAGTGCAATTATT
18 human 2146 EZH2 enhancer of zeste 2 polycom... XM_017011819.1 92.7% 92.4% 1_24del;916_917insGTAAGTGCAATTATT;1555_1556ins126
19 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515895.2 92.6% 92.6% 1_24del;243_244ins144
20 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515891.3 92.6% 90.9% (many diffs)
21 human 2146 EZH2 enhancer of zeste 2 polycom... NM_001203249.2 92.5% 92.2% 219_220ins27;865_866insGTAAGTGCAATTATT;1504_1505ins126
22 human 2146 EZH2 enhancer of zeste 2 polycom... XM_024446680.1 91.9% 90.1% (many diffs)
23 human 2146 EZH2 enhancer of zeste 2 polycom... XM_005249964.4 91.5% 91.3% (many diffs)
24 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515896.2 87.6% 87.3% (many diffs)
25 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515889.2 87.6% 86.6% (many diffs)
26 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515886.2 86% 87.2% (many diffs)
27 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515897.2 85.4% 85.4% 0_1ins327
28 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515898.2 85.4% 85.4% 0_1ins327
29 human 2146 EZH2 enhancer of zeste 2 polycom... XM_017011818.1 85.4% 86.4% (many diffs)
30 human 2146 EZH2 enhancer of zeste 2 polycom... XM_017011821.1 84.8% 84.6% 0_1ins327;565_566insGTAAGTGCAATTATT
31 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515899.3 66.1% 66.2% 1_24del;1531delC;1533_1534ins745
32 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515901.3 55.3% 54.1% (many diffs)
33 human 2146 EZH2 enhancer of zeste 2 polycom... XR_002956413.1 40.1% (many diffs)
34 human 2146 EZH2 enhancer of zeste 2 polycom... XR_001744581.1 38.5% 1_2639del;3878_3879ins265;4628_4893del
35 human 2146 EZH2 enhancer of zeste 2 polycom... XR_002956414.1 37.4% (many diffs)
36 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505520.3 92.5% 98.2% (many diffs)
37 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505521.2 92.5% 98.2% (many diffs)
38 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505526.3 92.5% 98.2% (many diffs)
39 mouse 14056 Ezh2 enhancer of zeste 2 polycom... NM_007971.2 91.9% 97.6% (many diffs)
40 mouse 14056 Ezh2 enhancer of zeste 2 polycom... NM_001146689.1 91.3% 97% (many diffs)
41 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505523.1 90.7% 96.4% (many diffs)
42 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505524.1 86.9% 92.5% (many diffs)
43 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505525.1 85.1% 90.6% (many diffs)
44 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505527.1 57.9% 54.3% (many diffs)
45 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505528.1 57.9% 54.3% (many diffs)
46 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505529.1 57.3% 54.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2319
ORF length:
2253
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGG CCAGACTGGG AAGAAATCTG AGAAGGGACC AGTTTGTTGG CGGAAGCGTG
121TAAAATCAGA GTACATGCGA CTGAGACAGC TCAAGAGGTT CAGACGAGCT GATGAAGTAA
181AGAGTATGTT TAGTTCCAAT CGTCAGAAAA TTTTGGAAAG AACGGAAATC TTAAACCAAG
241AATGGAAACA GCGAAGGATA CAGCCTGTGC ACATCCTGAC TTCTGTGAGC TCATTGCGCG
301GGACTAGGGA GTGTTCGGTG ACCAGTGACT TGGATTTTCC AACACAAGTC ATCCCATTAA
361AGACTCTGAA TGCAGTTGCT TCAGTACCCA TAATGTATTC TTGGTCTCCC CTACAGCAGA
421ATTTTATGGT GGAAGATGAA ACTGTTTTAC ATAACATTCC TTATATGGGA GATGAAGTTT
481TAGATCAGGA TGGTACTTTC ATTGAAGAAC TAATAAAAAA TTATGATGGG AAAGTACACG
541GGGATAGAGA ATGTGGGTTT ATAAATGATG AAATTTTTGT GGAGTTGGTG AATGCCCTTG
601GTCAATATAA TGATGATGAC GATGATGATG ATGGAGACGA TCCTGAAGAA AGAGAAGAAA
661AGCAGAAAGA TCTGGAGGAT CACCGAGATG ATAAAGAAAG CCGCCCACCT CGGAAATTTC
721CTTCTGATAA AATTTTTGAA GCCATTTCCT CAATGTTTCC AGATAAGGGC ACAGCAGAAG
781AACTAAAGGA AAAATATAAA GAACTCACCG AACAGCAGCT CCCAGGCGCA CTTCCTCCTG
841AATGTACCCC CAACATAGAT GGACCAAATG CTAAATCTGT TCAGAGAGAG CAAAGCTTAC
901ACTCCTTTCA TACGCTTTTC TGTAGGCGAT GTTTTAAATA TGACTGCTTC CTACATCGTA
961AGTGCAATTA TTCTTTTCAT GCAACACCCA ACACTTATAA GCGGAAGAAC ACAGAAACAG
1021CTCTAGACAA CAAACCTTGT GGACCACAGT GTTACCAGCA TTTGGAGGGA GCAAAGGAGT
1081TTGCTGCTGC TCTCACCGCT GAGCGGATAA AGACCCCACC AAAACGTCCA GGAGGCCGCA
1141GAAGAGGACG GCTTCCCAAT AACAGTAGCA GGCCCAGCAC CCCCACCATT AATGTGCTGG
1201AATCAAAGGA TACAGACAGT GATAGGGAAG CAGGGACTGA AACGGGGGGA GAGAACAATG
1261ATAAAGAAGA AGAAGAGAAG AAAGATGAAA CTTCGAGCTC CTCTGAAGCA AATTCTCGGT
1321GTCAAACACC AATAAAGATG AAGCCAAATA TTGAACCTCC TGAGAATGTG GAGTGGAGTG
1381GTGCTGAAGC CTCAATGTTT AGAGTCCTCA TTGGCACTTA CTATGACAAT TTCTGTGCCA
1441TTGCTAGGTT AATTGGGACC AAAACATGTA GACAGGTGTA TGAGTTTAGA GTCAAAGAAT
1501CTAGCATCAT AGCTCCAGCT CCCGCTGAGG ATGTGGATAC TCCTCCAAGG AAAAAGAAGA
1561GGAAACACCG GTTGTGGGCT GCACACTGCA GAAAGATACA GCTGAAAAAG GACGGCTCCT
1621CTAACCATGT TTACAACTAT CAACCCTGTG ATCATCCACG GCAGCCTTGT GACAGTTCGT
1681GCCCTTGTGT GATAGCACAA AATTTTTGTG AAAAGTTTTG TCAATGTAGT TCAGAGTGTC
1741AAAACCGCTT TCCGGGATGC CGCTGCAAAG CACAGTGCAA CACCAAGCAG TGCCCGTGCT
1801ACCTGGCTGT CCGAGAGTGT GACCCTGACC TCTGTCTTAC TTGTGGAGCC GCTGACCATT
1861GGGACAGTAA AAATGTGTCC TGCAAGAACT GCAGTATTCA GCGGGGCTCC AAAAAGCATC
1921TATTGCTGGC ACCATCTGAC GTGGCAGGCT GGGGGATTTT TATCAAAGAT CCTGTGCAGA
1981AAAATGAATT CATCTCAGAA TACTGTGGAG AGATTATTTC TCAAGATGAA GCTGACAGAA
2041GAGGGAAAGT GTATGATAAA TACATGTGCA GCTTTCTGTT CAACTTGAAC AATGATTTTG
2101TGGTGGATGC AACCCGCAAG GGTAACAAAA TTCGTTTTGC AAATCATTCG GTAAATCCAA
2161ACTGCTATGC AAAAGTTATG ATGGTTAACG GTGATCACAG GATAGGTATT TTTGCCAAGA
2221GAGCCATCCA GACTGGCGAA GAGCTGTTTT TTGATTACAG ATACAGCCAG GCTGATGCCC
2281TGAAGTATGT CGGCATCGAA AGAGAAATGG AAATCCCTTG CCCAACTTTC TTGTACAAAG
2341Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

Download FASTA (ORF) (Full)