Construct: ORF ccsbBroadEn_00933
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011243.1_s300c1, BRDN0000392595
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LDHB (3945)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3945 | LDHB | lactate dehydrogenase B | NM_001174097.2 | 100% | 100% | |
| 2 | human | 3945 | LDHB | lactate dehydrogenase B | NM_002300.7 | 100% | 100% | |
| 3 | human | 3945 | LDHB | lactate dehydrogenase B | NM_001315537.1 | 97.9% | 100% | 1003_1023del |
| 4 | human | 3945 | LDHB | lactate dehydrogenase B | XM_006719074.2 | 85% | 74.9% | (many diffs) |
| 5 | mouse | 16832 | Ldhb | lactate dehydrogenase B | NM_008492.3 | 86.3% | 97.9% | (many diffs) |
| 6 | mouse | 16832 | Ldhb | lactate dehydrogenase B | NM_001316322.1 | 84.5% | 97.9% | (many diffs) |
| 7 | mouse | 16832 | Ldhb | lactate dehydrogenase B | NM_001302765.1 | 67.3% | 70.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1068
- ORF length:
- 1002
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC AACTCTTAAG GAAAAACTCA TTGCACCAGT TGCGGAAGAA GAGGCAACAG 121 TTCCAAACAA TAAGATCACT GTAGTGGGTG TTGGACAAGT TGGTATGGCG TGTGCTATCA 181 GCATTCTGGG AAAGTCTCTG GCTGATGAAC TTGCTCTTGT GGATGTTTTG GAAGATAAGC 241 TTAAAGGAGA AATGATGGAT CTGCAGCATG GGAGCTTATT TCTTCAGACA CCTAAAATTG 301 TGGCAGATAA AGATTATTCT GTGACCGCCA ATTCTAAGAT TGTAGTGGTA ACTGCAGGAG 361 TCCGTCAGCA AGAAGGGGAG AGTCGGCTCA ATCTGGTGCA GAGAAATGTT AATGTCTTCA 421 AATTCATTAT TCCTCAGATC GTCAAGTACA GTCCTGATTG CATCATAATT GTGGTTTCCA 481 ACCCAGTGGA CATTCTTACG TATGTTACCT GGAAACTAAG TGGATTACCC AAACACCGCG 541 TGATTGGAAG TGGATGTAAT CTGGATTCTG CTAGATTTCG CTACCTTATG GCTGAAAAAC 601 TTGGCATTCA TCCCAGCAGC TGCCATGGAT GGATTTTGGG GGAACATGGC GACTCAAGTG 661 TGGCTGTGTG GAGTGGTGTG AATGTGGCAG GTGTTTCTCT CCAGGAATTG AATCCAGAAA 721 TGGGAACTGA CAATGATAGT GAAAATTGGA AGGAAGTGCA TAAGATGGTG GTTGAAAGTG 781 CCTATGAAGT CATCAAGCTA AAAGGATATA CCAACTGGGC TATTGGATTA AGTGTGGCTG 841 ATCTTATTGA ATCCATGTTG AAAAATCTAT CCAGGATTCA TCCCGTGTCA ACAATGGTAA 901 AGGGGATGTA TGGCATTGAG AATGAAGTCT TCCTGAGCCT TCCATGTATC CTCAATGCCC 961 GGGGATTAAC CAGCGTTATC AACCAGAAGC TAAAGGATGA TGAGGTTGCT CAGCTCAAGA 1021 AAAGTGCAGA TACCCTGTGG GACATCCAGA AGGACCTAAA AGACCTGTGC CCAACTTTCT 1081 TGTACAAAGT tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac