Construct: ORF ccsbBroadEn_00964

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000613.1_s300c1, BRDN0000456947
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SMAD7 (4092)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4092 SMAD7 SMAD family member 7 NM_005904.3 100% 100%
2 human 4092 SMAD7 SMAD family member 7 NM_001190821.1 99.7% 99.7% 666_667insGCA
3 human 4092 SMAD7 SMAD family member 7 NM_001190823.1 53.7% 48.2% (many diffs)
4 human 4092 SMAD7 SMAD family member 7 NM_001190822.2 49.5% 49.5% 0_1ins645
5 mouse 17131 Smad7 SMAD family member 7 NM_001042660.1 95.3% 98.1% (many diffs)
6 mouse 17131 Smad7 SMAD family member 7 XM_006525703.2 95.1% 97.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1347
ORF length:
1278
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GTTCAGGACC AAACGATCTG CGCTCGTCCG GCGTCTCTGG AGGAGCCGTG
121CGCCCGGCGG CGAGGACGAG GAGGAGGGCG CAGGGGGAGG TGGAGGAGGA GGCGAGCTGC
181GGGGAGAAGG GGCGACGGAC AGCCGAGCGC ATGGGGCCGG TGGCGGCGGC CCGGGCAGGG
241CTGGATGCTG CCTGGGCAAG GCGGTGCGAG GTGCCAAAGG TCACCACCAT CCCCACCCGC
301CAGCCGCGGG CGCCGGCGCG GCCGGGGGCG CCGAGGCGGA TCTGAAGGCG CTCACGCACT
361CGGTGCTCAA GAAACTGAAG GAGCGGCAGC TGGAGCTGCT GCTCCAGGCC GTGGAGTCCC
421GCGGCGGGAC GCGCACCGCG TGCCTCCTGC TGCCCGGCCG CCTGGACTGC AGGCTGGGCC
481CGGGGGCGCC CGCCGGCGCG CAGCCTGCGC AGCCGCCCTC GTCCTACTCG CTCCCCCTCC
541TGCTGTGCAA AGTGTTCAGG TGGCCGGATC TCAGGCATTC CTCGGAAGTC AAGAGGCTGT
601GTTGCTGTGA ATCTTACGGG AAGATCAACC CCGAGCTGGT GTGCTGCAAC CCCCATCACC
661TTAGCCGACT CTGCGAACTA GAGTCTCCCC CCCCTCCTTA CTCCAGATAC CCGATGGATT
721TTCTCAAACC AACTGCAGAC TGTCCAGATG CTGTGCCTTC CTCCGCTGAA ACAGGGGGAA
781CGAATTATCT GGCCCCTGGG GGGCTTTCAG ATTCCCAACT TCTTCTGGAG CCTGGGGATC
841GGTCACACTG GTGCGTGGTG GCATACTGGG AGGAGAAGAC GAGAGTGGGG AGGCTCTACT
901GTGTCCAGGA GCCCTCTCTG GATATCTTCT ATGATCTACC TCAGGGGAAT GGCTTTTGCC
961TCGGACAGCT CAATTCGGAC AACAAGAGTC AGCTGGTGCA GAAGGTGCGG AGCAAAATCG
1021GCTGCGGCAT CCAGCTGACG CGGGAGGTGG ATGGTGTGTG GGTGTACAAC CGCAGCAGTT
1081ACCCCATCTT CATCAAGTCC GCCACACTGG ACAACCCGGA CTCCAGGACG CTGTTGGTAC
1141ACAAGGTGTT CCCCGGTTTC TCCATCAAGG CTTTCGACTA CGAGAAGGCG TACAGCCTGC
1201AGCGGCCCAA TGACCACGAG TTTATGCAGC AGCCGTGGAC GGGCTTTACC GTGCAGATCA
1261GCTTTGTGAA GGGCTGGGGC CAGTGCTACA CCCGCCAGTT CATCAGCAGC TGCCCGTGCT
1321GGCTAGAGGT CATCTTCAAC AGCCGGTTGC CAACTTTCTT GTACAAAGTT GGcattataa
1381gaaagcattg cttatcaatt tgttgcaacg aac

Download FASTA (ORF) (Full)