Transcript: Human NM_001190822.2

Homo sapiens SMAD family member 7 (SMAD7), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-07-23
Taxon:
Homo sapiens (human)
Gene:
SMAD7 (4092)
Length:
2292
CDS:
138..773

Additional Resources:

NCBI RefSeq record:
NM_001190822.2
NBCI Gene record:
SMAD7 (4092)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001190822.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000019386 CGTGCAGATCAGCTTTGTGAA pLKO.1 674 CDS 100% 4.950 6.930 N SMAD7 n/a
2 TRCN0000222188 CGGCCCAATGACCACGAGTTT pLKO.1 627 CDS 100% 1.650 2.310 N SMAD7 n/a
3 TRCN0000428663 GTGTACAACCGCAGCAGTTAC pLKO_005 486 CDS 100% 10.800 8.640 N SMAD7 n/a
4 TRCN0000096052 ACAGCTCAATTCGGACAACAA pLKO.1 389 CDS 100% 4.950 3.960 N Smad7 n/a
5 TRCN0000428072 ACTACTTTGCTGCTAATATTT pLKO_005 821 3UTR 100% 15.000 10.500 N SMAD7 n/a
6 TRCN0000019388 CTCCAGATACCCGATGGATTT pLKO.1 125 5UTR 100% 10.800 7.560 N SMAD7 n/a
7 TRCN0000222190 GCTTTCAGATTCCCAACTTCT pLKO.1 227 CDS 100% 4.950 3.465 N SMAD7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001190822.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00964 pDONR223 100% 49.5% 49.5% None 0_1ins645 n/a
2 TRCN0000476798 TCAGATTCTGTTACAGAAGTAAAA pLX_317 24.7% 49.5% 49.5% V5 0_1ins645 n/a
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