Construct: ORF ccsbBroadEn_01036

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003122.1_s300c1, BRDN0000390450
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MSH5 (4439)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4439 MSH5 mutS homolog 5 NM_025259.5 100% 100%
2 human 4439 MSH5 mutS homolog 5 NM_172165.3 94.4% 94.4% 537_538ins51;2231_2320del
3 human 4439 MSH5 mutS homolog 5 NM_002441.4 94.3% 94.3% 537_538ins51;1959_1960insCAG;2228_2317del
4 human 4439 MSH5 mutS homolog 5 NM_172166.4 94.3% 94.3% 537_538ins51;1959_1960insCAG;2228_2317del
5 human 100532732 MSH5-SAPCD1 MSH5-SAPCD1 readthrough (NM... NR_037846.1 60.4% (many diffs)
6 mouse 17687 Msh5 mutS homolog 5 NM_001146215.2 81.8% 83.7% (many diffs)
7 mouse 17687 Msh5 mutS homolog 5 NM_013600.3 81.8% 83.7% (many diffs)
8 mouse 17687 Msh5 mutS homolog 5 XM_011246314.1 81.7% 83.6% (many diffs)
9 mouse 17687 Msh5 mutS homolog 5 XM_006523771.3 80.6% 81.7% (many diffs)
10 mouse 17687 Msh5 mutS homolog 5 XM_006523770.1 80.1% 81% (many diffs)
11 mouse 17687 Msh5 mutS homolog 5 XM_006523778.1 76.8% 78.5% (many diffs)
12 mouse 17687 Msh5 mutS homolog 5 XM_017317278.1 73.8% 75.8% (many diffs)
13 mouse 17687 Msh5 mutS homolog 5 XM_017317279.1 73.7% 75.7% (many diffs)
14 mouse 17687 Msh5 mutS homolog 5 XM_017317280.1 72.5% 74.4% (many diffs)
15 mouse 17687 Msh5 mutS homolog 5 XM_017317281.1 71.7% 73.7% (many diffs)
16 mouse 17687 Msh5 mutS homolog 5 XM_017317282.1 71.5% 73.4% (many diffs)
17 mouse 17687 Msh5 mutS homolog 5 NR_130640.1 70.8% (many diffs)
18 mouse 17687 Msh5 mutS homolog 5 XM_017317283.1 70% 71.1% (many diffs)
19 mouse 17687 Msh5 mutS homolog 5 XM_017317284.1 68.8% 70% (many diffs)
20 mouse 17687 Msh5 mutS homolog 5 NR_130641.1 68.2% (many diffs)
21 mouse 17687 Msh5 mutS homolog 5 XM_017317285.1 68% 69.4% (many diffs)
22 mouse 17687 Msh5 mutS homolog 5 XM_006523780.3 66.5% 69.2% (many diffs)
23 mouse 17687 Msh5 mutS homolog 5 XR_001782049.1 63.4% (many diffs)
24 mouse 17687 Msh5 mutS homolog 5 XR_001782050.1 62.7% (many diffs)
25 mouse 17687 Msh5 mutS homolog 5 XR_001782053.1 61.5% (many diffs)
26 mouse 17687 Msh5 mutS homolog 5 XR_001782051.1 58.1% (many diffs)
27 mouse 17687 Msh5 mutS homolog 5 XR_001782054.1 56.8% (many diffs)
28 mouse 17687 Msh5 mutS homolog 5 XM_017317286.1 56.5% 57.8% (many diffs)
29 mouse 17687 Msh5 mutS homolog 5 XR_001782052.1 52.9% (many diffs)
30 mouse 17687 Msh5 mutS homolog 5 XM_017317288.1 38.1% 36.6% (many diffs)
31 mouse 17687 Msh5 mutS homolog 5 XM_017317289.1 38.1% 36.6% (many diffs)
32 mouse 17687 Msh5 mutS homolog 5 XM_017317290.1 38.1% 36.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2532
ORF length:
2466
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC CTCCTTAGGA GCGAACCCAA GGAGGACACC GCAGGGACCG AGACCTGGGG
121CGGCCTCCTC CGGCTTCCCC AGCCCGGCCC CAGTGCCGGG CCCCAGGGAG GCCGAGGAGG
181AGGAAGTCGA GGAGGAGGAG GAGCTGGCCG AGATCCATCT GTGTGTGCTG TGGAATTCAG
241GATACTTGGG CATTGCCTAC TATGATACTA GTGACTCCAC TATCCACTTC ATGCCAGATG
301CCCCAGACCA CGAGAGCCTC AAGCTTCTCC AGAGAGTTCT GGATGAGATC AATCCCCAGT
361CTGTTGTTAC GAGTGCCAAA CAGGATGAGA ATATGACTCG ATTTCTGGGA AAGCTTGCCT
421CCCAGGAGCA CAGAGAGCCT AAAAGACCTG AAATCATATT TTTGCCAAGT GTGGATTTTG
481GTCTGGAGAT AAGCAAACAA CGCCTCCTTT CTGGAAACTA CTCCTTCATC CCAGACGCCA
541TGACTGCCAC TGAGAAAATC CTCTTCCTCT CTTCCATTAT TCCCTTTGAC TGCCTCCTCA
601CACCCCCAGG AGATTTAAGA TTTACCCCGA TTCCACTGCT GATCCCCTCC CAGGTTCGAG
661CACTTGGAGG GCTGCTGAAG TTCCTGGGTC GAAGAAGAAT CGGGGTTGAA CTGGAAGACT
721ATAATGTCAG CGTCCCCATC CTGGGCTTTA AGAAATTTAT GTTGACTCAT CTGGTGAACA
781TAGATCAAGA CACTTACAGT GTTCTACAGA TTTTTAAGAG TGAGTCTCAC CCCTCAGTGT
841ACAAAGTGGC CAGTGGACTG AAGGAGGGGC TCAGCCTCTT TGGAATCCTC AACAGATGCC
901ACTGTAAGTG GGGAGAGAAG CTGCTCAGGC TATGGTTCAC ACGTCCGACT CATGACCTGG
961GGGAGCTCAG TTCTCGTCTG GACGTCATTC AGTTTTTTCT GCTGCCCCAG AATCTGGACA
1021TGGCTCAGAT GCTGCATCGG CTCCTGGGTC ACATCAAGAA CGTGCCTCTG ATTCTGAAAC
1081GCATGAAGTT GTCCCACACC AAGGTCAGCG ACTGGCAGGT TCTCTACAAG ACTGTGTACA
1141GTGCCCTGGG CCTGAGGGAT GCCTGCCGCT CCCTGCCGCA GTCCATCCAG CTCTTTCGGG
1201ACATTGCCCA AGAGTTCTCT GATGACCTGC ACCATATCGC CAGCCTCATT GGGAAAGTAG
1261TGGACTTTGA GGGCAGCCTT GCTGAAAATC GCTTCACAGT CCTCCCCAAC ATAGATCCTG
1321AAATTGATGA GAAAAAGCGA AGACTGATGG GACTTCCCAG TTTCCTTACT GAGGTTGCCC
1381GCAAGGAGCT GGAGAATCTG GACTCCCGTA TTCCTTCATG CAGTGTCATC TACATCCCTC
1441TGATTGGCTT CCTTCTTTCT ATTCCCCGCC TGCCTTCCAT GGTAGAGGCC AGTGACTTTG
1501AGATTAATGG ACTGGACTTC ATGTTTCTCT CAGAGGAGAA GCTGCACTAT CGTAGTGCCC
1561GAACCAAGGA GCTGGATGCA TTGCTGGGGG ACCTGCACTG CGAGATCCGG GACCAGGAGA
1621CGCTGCTGAT GTACCAGCTA CAGTGCCAGG TGCTGGCACG AGCAGCTGTC TTAACCCGAG
1681TATTGGACCT TGCCTCCCGC CTGGACGTCC TGCTGGCTCT TGCCAGTGCT GCCCGGGACT
1741ATGGCTACTC AAGGCCGCGT TACTCCCCAC AAGTCCTTGG GGTACGAATC CAGAATGGCA
1801GACATCCTCT GATGGAACTC TGTGCCCGAA CCTTTGTGCC CAACTCCACA GAATGTGGTG
1861GGGACAAAGG GAGGGTCAAA GTCATCACTG GACCCAACTC ATCAGGGAAG AGCATATACC
1921TCAAACAGGT AGGCTTGATC ACATTCATGG CCCTGGTAGG CAGCTTTGTG CCAGCAGAGG
1981AGGCCGAAAT TGGGGCAGTA GACGCCATCT TCACACGAAT TCATAGCTGC GAATCCATCT
2041CCCTTGGCCT CTCCACCTTC ATGATCGACC TCAACCAGCA GGTGGCGAAA GCAGTGAACA
2101ATGCCACTGC ACAGTCGCTG GTCCTTATTG ATGAATTTGG AAAGGGAACC AACACGGTGG
2161ATGGGCTCGC GCTTCTGGCC GCTGTGCTCC GACACTGGCT GGCACGTGGA CCCACATGCC
2221CCCACATCTT TGTGGCCACC AACTTTCTGA GCCTTGTTCA GCTACAACTG CTGCCACAAG
2281GGCCCCTGGT GCAGTATTTG ACCATGGAGA CCTGTGAGGA TGGCAACGAT CTTGTCTTCT
2341TCTATCAGGT CTCAGACTTG ATCCGCAGTG GAAAACCCAT CAAGCCTGTC AAGGATTTGC
2401TAAAGAAGAA CCAAATGGAA AATTGCCAGA CATTAGTGGA TAAGTTTATG AAACTGGATT
2461TGGAAGATCC TAACCTGGAC TTGAACGTTT TCATGAGCCA GGAAGTGCTG CCTGCTGCCA
2521CCAGCATCCT CTGCCCAACT TTCTTGTACA AAGTtggcat tataagaaag cattgcttat
2581caatttgttg caacgaac

Download FASTA (ORF) (Full)