Construct: ORF ccsbBroadEn_01049
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003562.1_s300c1, BRDN0000398601
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTNR1A (4543)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4543 | MTNR1A | melatonin receptor 1A | NM_005958.4 | 100% | 100% | |
| 2 | human | 4543 | MTNR1A | melatonin receptor 1A | XM_011532002.3 | 75.7% | 75.7% | 0_1ins255 |
| 3 | mouse | 17773 | Mtnr1a | melatonin receptor 1A | XM_006509291.2 | 68.3% | 72% | (many diffs) |
| 4 | mouse | 17773 | Mtnr1a | melatonin receptor 1A | XM_011242176.1 | 68% | 72% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1119
- ORF length:
- 1050
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GCAGGGCAAC GGCAGCGCGC TGCCCAACGC CTCCCAGCCC GTGCTCCGCG 121 GGGACGGCGC GCGGCCCTCG TGGCTGGCGT CCGCCCTGGC CTGCGTCCTC ATCTTCACCA 181 TCGTGGTGGA CATCCTGGGC AACCTCCTGG TCATCCTGTC GGTGTATCGG AACAAGAAGC 241 TCAGGAACGC AGGAAACATC TTTGTGGTGA GCTTAGCGGT GGCAGACCTG GTGGTGGCCA 301 TTTATCCGTA CCCGTTGGTG CTGATGTCGA TATTTAACAA CGGGTGGAAC CTGGGCTATC 361 TGCACTGCCA AGTCAGTGGG TTCCTGATGG GCCTGAGCGT CATCGGCTCC ATATTCAACA 421 TCACCGGCAT CGCCATCAAC CGCTACTGCT ACATCTGCCA CAGTCTCAAG TACGACAAAC 481 TGTACAGCAG CAAGAACTCC CTCTGCTACG TGCTCCTCAT ATGGCTCCTG ACGCTGGCGG 541 CCGTCCTGCC CAACCTCCGT GCAGGGACTC TCCAGTACGA CCCGAGGATC TACTCGTGCA 601 CCTTCGCCCA GTCCGTCAGC TCCGCCTACA CCATCGCCGT GGTGGTTTTC CACTTCCTCG 661 TCCCCATGAT CATAGTCATC TTCTGTTACC TGAGAATATG GATCCTGGTT CTCCAGGTCA 721 GACAGAGGGT GAAACCTGAC CGCAAACCCA AACTGAAACC ACAGGACTTC AGGAATTTTG 781 TCACCATGTT TGTGGTTTTT GTCCTTTTTG CCATTTGCTG GGCTCCTCTG AACTTCATTG 841 GCCTGGCCGT GGCCTCTGAC CCCGCCAGCA TGGTGCCTAG GATCCCAGAG TGGCTGTTTG 901 TGGCCAGTTA CTACATGGCG TATTTCAACA GCTGCCTCAA TGCCATTATA TACGGGCTAC 961 TGAACCAAAA TTTCAGGAAG GAATACAGGA GAATTATAGT CTCGCTCTGT ACAGCCAGGG 1021 TGTTCTTTGT GGACAGCTCT AACGACGTGG CCGATAGGGT TAAATGGAAA CCGTCTCCAC 1081 TGATGACCAA CAATAATGTA GTAAAGGTGG ACTCCGTTTT GCCAACTTTC TTGTACAAAG 1141 Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac