Construct: ORF ccsbBroadEn_01162
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015147.1_s300c1, BRDN0000388279
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDGFB (5155)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5155 | PDGFB | platelet derived growth fac... | NM_002608.4 | 100% | 100% | |
| 2 | human | 5155 | PDGFB | platelet derived growth fac... | NM_033016.3 | 92.8% | 91.2% | (many diffs) |
| 3 | mouse | 18591 | Pdgfb | platelet derived growth fac... | NM_011057.3 | 86% | 89.6% | (many diffs) |
| 4 | mouse | 18591 | Pdgfb | platelet derived growth fac... | XM_017316502.1 | 86% | 89.6% | (many diffs) |
| 5 | mouse | 18591 | Pdgfb | platelet derived growth fac... | XM_006520592.3 | 62.5% | 65.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 790
- ORF length:
- 723
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAA 61 GTTGGCATGA ATCGCTGCTG GGCGCTCTTC CTGTCTCTCT GCTGCTACCT GCGTCTGGTC 121 AGCGCCGAGG GGGACCCCAT TCCCGAGGAG CTTTATGAGA TGCTGAGTGA CCACTCGATC 181 CGCTCCTTTG ATGATCTCCA ACGCCTGCTG CACGGAGACC CCGGAGAGGA AGATGGGGCC 241 GAGTTGGACC TGAACATGAC CCGCTCCCAC TCTGGAGGCG AGCTGGAGAG CTTGGCTCGT 301 GGAAGAAGGA GCCTGGGTTC CCTGACCATT GCTGAGCCGG CCATGATCGC CGAGTGCAAG 361 ACGCGCACCG AGGTGTTCGA GATCTCCCGG CGCCTCATAG ACCGCACCAA CGCCAACTTC 421 CTGGTGTGGC CGCCCTGTGT GGAGGTGCAG CGCTGCTCCG GCTGCTGCAA CAACCGCAAC 481 GTGCAGTGCC GCCCCACCCA GGTGCAGCTG CGACCTGTCC AGGTGAGAAA GATCGAGATT 541 GTGCGGAAGA AGCCAATCTT TAAGAAGGCC ACGGTGACGC TGGAAGACCA CCTGGCATGC 601 AAGTGTGAGA CAGTGGCAGC TGCACGGCCT GTGACCCGAA GCCCGGGGGG TTCCCAGGAG 661 CAGCGAGCCA AAACGCCCCA AACTCGGGTG ACCATTCGGA CGGTGCGAGT CCGCCGGCCC 721 CCCAAGGGCA AGCACCGGAA ATTCAAGCAC ACGCATGACA AGACGGCACT GAAGGAGACC 781 CTTGGAGCCT ACCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca 841 atttgttgca acgaac