Construct: ORF ccsbBroadEn_01177
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013902.1_s300c1, BRDN0000396895
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PFDN1 (5201)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5201 | PFDN1 | prefoldin subunit 1 | NM_002622.5 | 100% | 100% | |
| 2 | human | 5201 | PFDN1 | prefoldin subunit 1 | XM_005268465.4 | 80.8% | 81.9% | (many diffs) |
| 3 | mouse | 67199 | Pfdn1 | prefoldin 1 | NM_026027.3 | 91.2% | 97.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 432
- ORF length:
- 366
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC CGCCCCCGTG GATCTAGAGC TGAAGAAGGC CTTCACAGAG CTTCAAGCCA 121 AAGTTATTGA CACTCAACAG AAGGTGAAGC TCGCAGACAT ACAGATTGAA CAGCTAAACA 181 GAACGAAAAA GCATGCACAT CTTACAGATA CAGAGATCAT GACTTTGGTA GATGAGACTA 241 ACATGTATGA AGGTGTAGGA AGAATGTTTA TTCTTCAGTC CAAGGAAGCA ATTCACAGTC 301 AGCTGTTAGA GAAGCAGAAA ATAGCAGAAG AAAAAATTAA AGAACTAGAA CAGAAAAAGT 361 CCTACCTGGA GCGAAGCGTT AAGGAAGCTG AGGACAACAT CCGGGAGATG CTGATGGCAC 421 GAAGGGCCCA GTACCCAACT TTCTTGTACA AAGTtggcat tataagaaag cattgcttat 481 caatttgttg caacgaac