Construct: ORF ccsbBroadEn_01186

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002684.1_s300c1, BRDN0000392238
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PHB (5245)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5245 PHB prohibitin NM_001281496.1 100% 100%
2 human 5245 PHB prohibitin NM_001281715.1 100% 100%
3 human 5245 PHB prohibitin NM_002634.4 100% 100%
4 human 5245 PHB prohibitin XM_017024763.1 100% 100%
5 human 5245 PHB prohibitin NM_001281497.1 54.7% 56.9% (many diffs)
6 mouse 18673 Phb prohibitin NM_008831.4 91.1% 99.6% (many diffs)
7 mouse 237880 1700071K01Rik RIKEN cDNA 1700071K01 gene NM_001033765.2 88.9% 92.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
882
ORF length:
816
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC TGCCAAAGTG TTTGAGTCCA TTGGCAAGTT TGGCCTGGCC TTAGCTGTTG
121CAGGAGGCGT GGTGAACTCT GCCTTATATA ATGTGGATGC TGGGCACAGA GCTGTCATCT
181TTGACCGATT CCGTGGAGTG CAGGACATTG TGGTAGGGGA AGGGACTCAT TTTCTCATCC
241CGTGGGTACA GAAACCAATT ATCTTTGACT GCCGTTCTCG ACCACGTAAT GTGCCAGTCA
301TCACTGGTAG CAAAGATTTA CAGAATGTCA ACATCACACT GCGCATCCTC TTCCGGCCTG
361TCGCCAGCCA GCTTCCTCGC ATCTTCACCA GCATCGGAGA GGACTATGAT GAGCGTGTGC
421TGCCGTCCAT CACAACTGAG ATCCTCAAGT CAGTGGTGGC TCGCTTTGAT GCTGGAGAAC
481TAATCACCCA GAGAGAGCTG GTCTCCAGGC AGGTGAGCGA CGACCTTACA GAGCGAGCCG
541CCACCTTTGG GCTCATCCTG GATGACGTGT CCTTGACACA TCTGACCTTC GGGAAGGAGT
601TCACAGAAGC GGTGGAAGCC AAACAGGTGG CTCAGCAGGA AGCAGAGAGG GCCAGATTTG
661TGGTGGAAAA GGCTGAGCAA CAGAAAAAGG CGGCCATCAT CTCTGCTGAG GGCGACTCCA
721AGGCAGCTGA GCTGATTGCC AACTCACTGG CCACTGCAGG GGATGGCCTG ATCGAGCTGC
781GCAAGCTGGA AGCTGCAGAG GACATCGCGT ACCAGCTCTC ACGCTCTCGG AACATCACCT
841ACCTGCCAGC GGGGCAGTCC GTGCTCCTCC AGCTGCCCCA GTGCCCAACT TTCTTGTACA
901AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac

Download FASTA (ORF) (Full)