Construct: ORF ccsbBroadEn_01271

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006752.1_s300c1, BRDN0000396458
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PPT1 (5538)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5538 PPT1 palmitoyl-protein thioester... NM_000310.3 100% 100%
2 human 5538 PPT1 palmitoyl-protein thioester... NM_001363695.2 92.1% 92.1% 724_725ins72
3 human 5538 PPT1 palmitoyl-protein thioester... NM_001142604.2 66.3% 66.3% 123_124ins309
4 mouse 19063 Ppt1 palmitoyl-protein thioester... NM_008917.3 84.8% 85.2% (many diffs)
5 mouse 19063 Ppt1 palmitoyl-protein thioester... XR_376299.3 27.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
984
ORF length:
918
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GTCGCCCGGC TGCCTGTGGC TCTTGGCTGT GGCTCTCCTG CCATGGACCT
121GCGCTTCTCG GGCGCTGCAG CATCTGGACC CGCCGGCGCC GCTGCCGTTG GTGATCTGGC
181ATGGGATGGG AGACAGCTGT TGCAATCCCT TAAGCATGGG TGCTATTAAA AAAATGGTGG
241AGAAGAAAAT ACCTGGAATT TACGTCTTAT CTTTAGAGAT TGGGAAGACC CTGATGGAGG
301ACGTGGAGAA CAGCTTCTTC TTGAATGTCA ATTCCCAAGT AACAACAGTG TGTCAGGCAC
361TTGCTAAGGA TCCTAAATTG CAGCAAGGCT ACAATGCTAT GGGATTCTCC CAGGGAGGCC
421AATTTCTGAG GGCAGTGGCT CAGAGATGCC CTTCACCTCC CATGATCAAT CTGATCTCGG
481TTGGGGGACA ACATCAAGGT GTTTTTGGAC TCCCTCGATG CCCAGGAGAG AGCTCTCACA
541TCTGTGACTT CATCCGAAAA ACACTGAATG CTGGGGCGTA CTCCAAAGTT GTTCAGGAAC
601GCCTCGTGCA AGCCGAATAC TGGCATGACC CCATAAAGGA GGATGTGTAT CGCAACCACA
661GCATCTTCTT GGCAGATATA AATCAGGAGC GGGGTATCAA TGAGTCCTAC AAGAAAAACC
721TGATGGCCCT GAAGAAGTTT GTGATGGTGA AATTCCTCAA TGATTCCATT GTGGACCCTG
781TAGATTCGGA GTGGTTTGGA TTTTACAGAA GTGGCCAAGC CAAGGAAACC ATTCCCTTAC
841AGGAGACCTC CCTGTACACA CAGGACCGCC TGGGGCTAAA GGAAATGGAC AATGCAGGAC
901AGCTAGTGTT TCTGGCTACA GAAGGGGACC ATCTTCAGTT GTCTGAAGAA TGGTTTTATG
961CCCACATCAT ACCATTCCTT GGATGCCCAA CTTTCTTGTA CAAAGTtggc attataagaa
1021agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)