Construct: ORF ccsbBroadEn_01311

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017640.1_s300c1, BRDN0000388845
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PSMB3 (5691)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5691 PSMB3 proteasome 20S subunit beta 3 NM_002795.4 100% 100%
2 human 5691 PSMB3 proteasome 20S subunit beta 3 NR_104195.2 70.6% 1_86del;381_382ins77;625_685del
3 human 5691 PSMB3 proteasome 20S subunit beta 3 NR_104194.2 57.3% 1_86del;381_382ins178;524_584del
4 mouse 26446 Psmb3 proteasome (prosome, macrop... NM_011971.4 91% 98.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
681
ORF length:
615
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTCGCATGTC TATTATGTCC TATAACGGAG GGGCCGTCAT GGCCATGAAG GGGAAGAACT
121GTGTGGCCAT CGCTGCAGAC AGGCGCTTCG GGATCCAGGC CCAGATGGTG ACCACGGACT
181TCCAGAAGAT CTTTCCCATG GGTGACCGGC TGTACATCGG TCTGGCCGGG CTCGCCACTG
241ACGTCCAGAC AGTTGCCCAG CGCCTCAAGT TCCGGCTGAA CCTGTATGAG TTGAAGGAAG
301GTCGGCAGAT CAAACCTTAT ACCCTCATGA GCATGGTGGC CAACCTCTTG TATGAGAAAC
361GGTTTGGCCC TTACTACACT GAGCCAGTCA TTGCCGGGTT GGACCCGAAG ACCTTTAAGC
421CCTTCATTTG CTCTCTAGAC CTCATCGGCT GCCCCATGGT GACTGATGAC TTTGTGGTCA
481GTGGCACCTG CGCCGAACAA ATGTACGGAA TGTGTGAGTC CCTCTGGGAG CCCAACATGG
541ATCCGGATCA CCTGTTTGAA ACCATCTCCC AAGCCATGCT GAATGCTGTG GACCGGGATG
601CAGTGTCAGG CATGGGAGTC ATTGTCCACA TCATCGAGAA GGACAAAATC ACCACCAGGA
661CACTGAAGGC CCGAATGGAC TACCCAACTT TCTTGTACAA AGTtggcatt ataagaaagc
721attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)