Construct: ORF ccsbBroadEn_01314

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014434.1_s300c1, BRDN0000389220
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PSMC2 (5701)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5701 PSMC2 proteasome 26S subunit, ATP... NM_002803.4 99.9% 100% 934A>C
2 human 5701 PSMC2 proteasome 26S subunit, ATP... XR_002956469.1 37.2% (many diffs)
3 human 5701 PSMC2 proteasome 26S subunit, ATP... XR_002956468.1 36% 1_309del;731_1327del;1702_1703ins503
4 human 5701 PSMC2 proteasome 26S subunit, ATP... NM_001204453.1 32.4% 32.5% 423_423delGins877
5 mouse 19181 Psmc2 proteasome (prosome, macrop... NM_011188.3 80.9% 90.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1365
ORF length:
1299
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCC GGATTACCTC GGTGCCGATC AGCGGAAGAC CAAAGAGGAT GAGAAGGACG
121ACAAGCCCAT CCGAGCTCTG GATGAGGGGG ATATTGCCTT GTTGAAAACT TATGGTCAGA
181GCACTTACTC TAGGCAGATC AAGCAAGTTG AAGATGACAT TCAGCAACTT CTCAAGAAAA
241TTAATGAGCT CACTGGTATT AAAGAATCTG ACACTGGCCT GGCCCCACCA GCACTCTGGG
301ATTTGGCTGC AGATAAGCAG ACACTCCAGA GTGAACAGCC TTTACAGGTT GCCAGGTGTA
361CAAAGATAAT CAATGCTGAT TCGGAGGACC CAAAATACAT TATCAACGTA AAGCAGTTTG
421CCAAGTTTGT GGTGGACCTT AGTGATCAGG TGGCACCTAC TGACATTGAA GAAGGGATGA
481GAGTGGGCGT GGATAGAAAT AAATATCAAA TTCACATTCC ATTGCCTCCT AAGATTGACC
541CAACAGTTAC CATGATGCAG GTGGAAGAGA AACCTGATGT CACATACAGT GATGTTGGTG
601GCTGTAAGGA ACAGATTGAG AAACTGCGAG AAGTAGTTGA AACCCCATTA CTTCATCCAG
661AGAGGTTTGT GAACCTTGGC ATTGAGCCTC CCAAGGGCGT GCTGCTCTTT GGTCCACCCG
721GTACAGGCAA GACACTCTGT GCGCGGGCAG TTGCTAATCG GACTGATGCG TGCTTCATTC
781GAGTTATTGG ATCTGAGCTT GTACAGAAAT ACGTCGGTGA GGGGGCTCGA ATGGTTCGTG
841AACTCTTTGA AATGGCCAGA ACAAAAAAAG CCTGCCTTAT CTTCTTTGAT GAAATTGATG
901CTATTGGAGG GGCTCGTTTT GATGATGGTG CTGGAGGTGA CAATGAAGTG CAGAGAACAA
961TGTTGGAACT GATCAATCAG CTTGATGGTT TTGATCCTCG AGGCAATATT AAAGTGCTGA
1021TGGCCACTAA CAGACCTGAT ACTTTGGATC CAGCACTGAT GAGGCCAGGG AGATTGGATA
1081GAAAAATTGA ATTTAGCTTG CCCGATCTAG AGGGTCGGAC CCACATATTT AAGATTCACG
1141CTCGTTCAAT GAGTGTTGAA AGAGATATCA GATTTGAACT GTTAGCACGA CTGTGTCCAA
1201ATAGCACTGG TGCTGAGATT AGAAGCGTCT GCACAGAGGC TGGTATGTTT GCCATCAGAG
1261CACGGCGAAA AATTGCTACC GAGAAGGATT TCTTGGAAGC TGTAAATAAG GTCATTAAGT
1321CTTATGCCAA ATTCAGTGCT ACTCCTCGTT ACATGACATA CAACTGCCCA ACTTTCTTGT
1381ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)