Construct: ORF ccsbBroadEn_01370

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007677.1_s300c1, BRDN0000392542
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RAD51D (5892)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5892 RAD51D RAD51 paralog D NM_002878.3 100% 100%
2 human 5892 RAD51D RAD51 paralog D NM_001142571.2 89.9% 83.4% (many diffs)
3 human 5892 RAD51D RAD51 paralog D NM_133629.3 65.8% 65.8% 142_143ins336
4 human 100529207 RAD51L3-RFFL RAD51L3-RFFL readthrough NR_037714.1 15.5% (many diffs)
5 human 5892 RAD51D RAD51 paralog D NR_037711.2 8.7% 1_145del;287_288ins119;1011_9745del
6 human 5892 RAD51D RAD51 paralog D NR_037712.2 7.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1050
ORF length:
984
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGG CGTGCTCAGG GTCGGACTGT GCCCTGGCCT TACCGAGGAG ATGATCCAGC
121TTCTCAGGAG CCACAGGATC AAGACAGTGG TGGACCTGGT TTCTGCAGAC CTGGAAGAGG
181TAGCTCAGAA ATGTGGCTTG TCTTACAAGG CCCTGGTTGC CCTGAGGCGG GTGCTGCTGG
241CTCAGTTCTC GGCTTTCCCC GTGAATGGCG CTGATCTCTA CGAGGAACTG AAGACCTCCA
301CTGCCATCCT GTCCACTGGC ATTGGCAGTC TTGATAAACT GCTTGATGCT GGTCTCTATA
361CTGGAGAAGT GACTGAAATT GTAGGAGGCC CAGGTAGCGG CAAAACTCAG GTATGTCTCT
421GTATGGCAGC AAATGTGGCC CATGGCCTGC AGCAAAACGT CCTATATGTA GATTCCAATG
481GAGGGCTGAC AGCTTCCCGC CTCCTCCAGC TGCTTCAGGC TAAAACCCAG GATGAGGAGG
541AACAGGCAGA AGCTCTCCGG AGGATCCAGG TGGTGCATGC ATTTGACATC TTCCAGATGC
601TGGATGTGCT GCAGGAGCTC CGAGGCACTG TGGCCCAGCA GGTGACTGGT TCTTCAGGAA
661CTGTGAAGGT GGTGGTTGTG GACTCGGTCA CTGCGGTGGT TTCCCCACTT CTGGGAGGTC
721AGCAGAGGGA AGGCTTGGCC TTGATGATGC AGCTGGCCCG AGAGCTGAAG ACCCTGGCCC
781GGGACCTTGG CATGGCAGTG GTGGTGACCA ACCACATAAC TCGAGACAGG GACAGCGGGA
841GGCTCAAACC TGCCCTCGGA CGCTCCTGGA GCTTTGTGCC CAGCACTCGG ATTCTCCTGG
901ACACCATCGA GGGAGCAGGA GCATCAGGCG GCCGGCGCAT GGCGTGTCTG GCCAAATCTT
961CCCGACAGCC AACAGGTTTC CAGGAGATGG TAGACATTGG GACCTGGGGG ACCTCAGAGC
1021AGAGTGCCAC ATTACAGGGT GATCAGACAT GCCCAACTTT CTTGTACAAA GTtggcatta
1081taagaaagca ttgcttatca atttgttgca acgaac

Download FASTA (ORF) (Full)