Construct: ORF ccsbBroadEn_01433
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006535.1_s300c1, BRDN0000388719
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL31 (6160)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6160 | RPL31 | ribosomal protein L31 | NM_000993.5 | 100% | 100% | |
| 2 | human | 6160 | RPL31 | ribosomal protein L31 | NM_001098577.2 | 93.3% | 92.1% | (many diffs) |
| 3 | human | 6160 | RPL31 | ribosomal protein L31 | NM_001099693.1 | 93.1% | 92% | (many diffs) |
| 4 | human | 641311 | RPL31P11 | ribosomal protein L31 pseud... | NR_002595.1 | 22.2% | (many diffs) | |
| 5 | mouse | 114641 | Rpl31 | ribosomal protein L31 | NM_001252218.1 | 90.9% | 100% | (many diffs) |
| 6 | mouse | 114641 | Rpl31 | ribosomal protein L31 | NM_001252219.1 | 90.9% | 100% | (many diffs) |
| 7 | mouse | 114641 | Rpl31 | ribosomal protein L31 | NM_053257.3 | 90.9% | 100% | (many diffs) |
| 8 | mouse | 665562 | Rpl31-ps12 | ribosomal protein L31, pseu... | NM_001258458.1 | 77.9% | 84.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 441
- ORF length:
- 375
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC TCCCGCAAAG AAGGGTGGCG AGAAGAAAAA GGGCCGTTCT GCCATCAACG 121 AAGTGGTAAC CCGAGAATAC ACCATCAACA TTCACAAGCG CATCCATGGA GTGGGCTTCA 181 AGAAGCGTGC ACCTCGGGCA CTCAAAGAGA TTCGGAAATT TGCCATGAAG GAGATGGGAA 241 CTCCAGATGT GCGCATTGAC ACCAGGCTCA ACAAAGCTGT CTGGGCCAAA GGAATAAGGA 301 ATGTGCCATA CCGAATCCGT GTGCGGCTGT CCAGAAAACG TAATGAGGAT GAAGATTCAC 361 CAAATAAGCT ATATACTTTG GTTACCTATG TACCTGTTAC CACTTTCAAA AATCTACAGA 421 CAGTCAATGT GGATGAGAAC TACCCAACTT TCTTGTACAA AGTtggcatt ataagaaagc 481 attgcttatc aatttgttgc aacgaac