Construct: ORF ccsbBroadEn_01469

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005295.1_s300c1, BRDN0000395355
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RSU1 (6251)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6251 RSU1 Ras suppressor protein 1 NM_012425.4 100% 100%
2 human 6251 RSU1 Ras suppressor protein 1 NM_152724.2 80.8% 80.8% 0_1ins159
3 human 6251 RSU1 Ras suppressor protein 1 XM_005252552.4 72.4% 68.1% 598_599ins133;699_831del
4 mouse 20163 Rsu1 Ras suppressor protein 1 NM_009105.4 88.5% 97.1% (many diffs)
5 mouse 20163 Rsu1 Ras suppressor protein 1 XM_006497405.3 83.5% 91.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
897
ORF length:
831
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTC CAAGTCTCTG AAGAAGTTGG TGGAGGAGAG CCGGGAGAAG AACCAGCCCG
121AGGTGGACAT GAGTGACCGG GGCATCTCCA ACATGCTGGA TGTCAACGGC CTCTTTACCT
181TATCCCATAT CACACAACTG GTCCTCAGCC ATAACAAGCT AACAATGGTG CCACCGAACA
241TCGCAGAACT GAAGAATTTG GAGGTGCTCA ACTTTTTTAA TAACCAAATC GAGGAGCTGC
301CCACACAGAT CAGTAGCCTT CAGAAACTCA AACACCTGAA CCTTGGCATG AACAGGCTGA
361ACACTTTGCC ACGAGGCTTC GGCTCCCTGC CAGCTCTTGA GGTTCTGGAC TTGACGTACA
421ACAACTTGAG CGAAAATTCT CTTCCTGGAA ACTTCTTCTA CCTGACCACC CTGCGTGCAC
481TCTATCTAAG TGACAACGAT TTTGAAATCC TGCCGCCAGA TATTGGGAAG CTCACAAAGT
541TGCAGATACT CAGCCTTAGG GATAACGACC TGATCTCGCT GCCTAAGGAA ATCGGGGAGC
601TTACCCAGCT TAAAGAGCTC CACATTCAGG GGAACCGCCT CACCGTTCTG CCCCCAGAAC
661TAGGAAACTT GGATTTAACT GGCCAGAAGC AGGTATTCAA AGCAGAGAAC AATCCCTGGG
721TGACCCCCAT TGCAGACCAG TTCCAGCTTG GCGTGTCCCA TGTTTTTGAG TATATCCGTT
781CTGAGACATA CAAATACCTC TACGGCAGAC ACATGCAGGC CAACCCAGAA CCACCGAAGA
841AGAATAATGA CAAATCGAAA AAGATCAGCC GGAAACCCCT GGCAGCCAAG AACAGATACC
901CAACTTTCTT GTACAAAGTt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
961aac

Download FASTA (ORF) (Full)