Construct: ORF ccsbBroadEn_01474
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009773.1_s300c1, BRDN0000388644
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- S100A4 (6275)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6275 | S100A4 | S100 calcium binding protei... | NM_002961.3 | 100% | 100% | |
| 2 | human | 6275 | S100A4 | S100 calcium binding protei... | NM_019554.3 | 100% | 100% | |
| 3 | mouse | 20198 | S100a4 | S100 calcium binding protei... | NM_011311.2 | 89.8% | 93% | (many diffs) |
| 4 | mouse | 20198 | S100a4 | S100 calcium binding protei... | XM_006501177.3 | 89.8% | 93% | (many diffs) |
| 5 | mouse | 20198 | S100a4 | S100 calcium binding protei... | XM_006501178.2 | 89.8% | 93% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 369
- ORF length:
- 303
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC GTGCCCTCTG GAGAAGGCCC TGGATGTGAT GGTGTCCACC TTCCACAAGT 121 ACTCGGGCAA AGAGGGTGAC AAGTTCAAGC TCAACAAGTC AGAACTAAAG GAGCTGCTGA 181 CCCGGGAGCT GCCCAGCTTC TTGGGGAAAA GGACAGATGA AGCTGCTTTC CAGAAGCTGA 241 TGAGCAACTT GGACAGCAAC AGGGACAACG AGGTGGACTT CCAAGAGTAC TGTGTCTTCC 301 TGTCCTGCAT CGCCATGATG TGTAACGAAT TCTTTGAAGG CTTCCCAGAT AAGCAGCCCA 361 GGAAGAAATG CCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa 421 tttgttgcaa cgaac