Construct: ORF ccsbBroadEn_01578

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011324.1_s300c1, BRDN0000395101
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SOS2 (6655)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6655 SOS2 SOS Ras/Rho guanine nucleot... NM_006939.4 100% 100%
2 mouse 20663 Sos2 son of sevenless homolog 2 ... XM_006515635.1 88.5% 94.9% (many diffs)
3 mouse 20663 Sos2 son of sevenless homolog 2 ... NM_001135559.1 88.5% 94.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4065
ORF length:
3996
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GCAGCAGGCG CCGCAGCCTT ACGAGTTCTT CAGCGAGGAG AACAGTCCGA
121AATGGCGGGG ACTGTTGGTC TCGGCCCTGC GGAAGGTTCA GGAACAAGTG CATCCCACTC
181TCTCAGCTAA TGAAGAGTCT CTCTATTATA TTGAAGAGCT GATTTTTCAG CTGCTTAATA
241AATTATGCAT GGCCCAGCCA AGGACTGTTC AAGATGTAGA GGAGCGAGTT CAGAAGACCT
301TTCCTCACCC AATTGATAAA TGGGCCATTG CTGATGCACA ATCTGCTATA GAAAAACGAA
361AACGAAGAAA TCCTCTTTTA CTGCCTGTGG ACAAAATCCA TCCTTCGTTG AAGGAAGTAT
421TAGGGTACAA AGTGGACTAC CATGTATCCC TATATATTGT GGCTGTACTA GAGTATATCT
481CAGCTGATAT TTTAAAATTG GCTGGTAATT ATGTTTTTAA TATCCGGCAT TATGAAATAT
541CTCAGCAGGA CATTAAAGTG TCAATGTGTG CGGATAAGGT TTTGATGGAC ATGTTTGATC
601AGGATGACAT AGGTTTGGTT TCTCTCTGTG AAGATGAACC TAGTTCTTCT GGTGAATTAA
661ACTACTATGA TCTTGTCAGA ACTGAAATCG CAGAAGAAAG ACAGTATCTA CGGGAATTAA
721ATATGATCAT AAAAGTGTTT CGAGAAGCCT TTCTTTCTGA TAGAAAGCTG TTTAAACCTT
781CTGATATCGA AAAGATTTTT AGTAACATTT CAGATATACA TGAATTGACT GTGAAACTTT
841TAGGTTTGAT TGAAGACACA GTTGAAATGA CTGATGAAAG CAGTCCTCAT CCCTTAGCTG
901GCAGCTGTTT TGAAGATTTG GCAGAAGAGC AAGCATTTGA TCCTTATGAA ACATTATCAC
961AGGACATTCT TTCACCAGAG TTTCATGAAC ATTTCAATAA ATTGATGGCC AGACCTGCAG
1021TTGCTCTACA CTTTCAGTCC ATTGCTGATG GTTTTAAAGA GGCAGTTCGT TATGTCCTTC
1081CACGTCTTAT GCTGGTGCCA GTGTATCACT GTTGGCACTA CTTTGAGTTA CTAAAGCAAT
1141TGAAAGCATG TAGTGAAGAA CAAGAAGACA GAGAATGTTT GAACCAAGCT ATTACTGCTC
1201TCATGAATCT CCAAGGTAGC ATGGACCGAA TTTACAAGCA GTATTCACCT AGACGTCGAC
1261CTGGAGATCC TGTTTGCCCT TTTTATAGTC ACCAATTAAG AAGCAAACAC CTGGCTATCA
1321AAAAAATGAA TGAAATTCAG AAAAATATCG ATGGATGGGA AGGCAAAGAT ATTGGACAGT
1381GTTGTAATGA ATTCATTATG GAGGGACCAT TGACAAGAAT CGGTGCCAAA CATGAACGGC
1441ATATTTTTCT GTTTGATGGC TTAATGATCA GTTGTAAACC TAATCATGGC CAGACTCGGC
1501TTCCAGGTTA CAGTAGTGCA GAATACAGGT TAAAAGAAAA ATTTGTCATG AGGAAAATAC
1561AAATTTGTGA TAAAGAAGAT ACTTGTGAGC ACAAGCATGC ATTTGAATTA GTATCCAAAG
1621ATGAGAACAG CATAATATTT GCTGCTAAGT CTGCTGAAGA AAAAAACAAC TGGATGGCAG
1681CCCTTATTTC TCTTCATTAT CGTAGTACTC TAGATCGAAT GTTAGATTCA GTATTATTGA
1741AAGAAGAAAA TGAGCAACCA CTGAGATTAC CAAGTCCTGA AGTATATCGT TTTGTAGTAA
1801AAGACTCTGA GGAAAACATT GTTTTTGAAG ACAACTTGCA AAGTAGAAGT GGCATCCCCA
1861TTATTAAAGG AGGAACTGTA GTGAAATTAA TTGAAAGGTT AACATATCAT ATGTATGCAG
1921ATCCCAATTT TGTTCGTACT TTTCTTACCA CATATCGTTC ATTTTGTAAA CCACAGGAAT
1981TGCTGAGCTT ACTGATTGAA CGGTTTGAAA TTCCAGAGCC AGAACCTACT GACGCAGACA
2041AATTGGCAAT AGAGAAAGGC GAGCAGCCAA TCAGTGCAGA CCTTAAAAGA TTTCGCAAGG
2101AATATGTCCA ACCAGTACAA CTTAGGATCT TAAATGTATT TCGGCATTGG GTTGAACATC
2161ATTTTTATGA CTTTGAAAGA GACTTGGAAT TGCTTGAAAG ACTAGAATCC TTCATTTCAA
2221GTGTAAGAGG GAAAGCTATG AAAAAATGGG TAGAGTCAAT TGCTAAGATC ATCAGGAGGA
2281AGAAGCAAGC TCAGGCAAAC GGAGTAAGCC ATAATATTAC CTTTGAAAGT CCACCTCCAC
2341CAATTGAATG GCATATCAGC AAACCAGGAC AGTTTGAAAC ATTTGATCTC ATGACACTTC
2401ATCCAATAGA AATTGCACGT CAGCTGACAC TTTTGGAGTC TGATCTTTAC AGGAAAGTTC
2461AACCGTCTGA ACTTGTAGGG AGTGTGTGGA CCAAAGAAGA TAAAGAAATA AATTCTCCAA
2521ATTTATTAAA AATGATTCGC CATACCACAA ATCTCACCCT CTGGTTTGAA AAATGCATTG
2581TGGAAGCAGA AAATTTTGAA GAACGGGTGG CAGTACTAAG TAGAATTATA GAAATTCTGC
2641AAGTTTTTCA AGATTTGAAT AATTTCAATG GCGTATTGGA GATAGTCAGT GCAGTAAATT
2701CAGTGTCAGT ATACAGACTA GACCATACCT TTGAGGCACT GCAGGAAAGG AAAAGGAAAA
2761TTTTGGACGA AGCTGTGGAA TTAAGTCAAG ATCACTTTAA AAAATACCTA GTAAAACTTA
2821AGTCAATCAA TCCACCTTGT GTGCCTTTTT TTGGAATATA TTTAACAAAT ATTCTGAAGA
2881CCGAAGAAGG GAATAATGAT TTTTTAAAAA AGAAAGGGAA AGATTTAATC AATTTCAGTA
2941AGAGGAGGAA AGTAGCTGAA ATTACTGGAG AAATTCAGCA GTATCAGAAT CAGCCTTACT
3001GTTTACGGAT AGAACCAGAT ATGAGGAGAT TCTTTGAAAA CCTTAACCCC ATGGGAAGTG
3061CATCTGAAAA AGAGTTTACA GATTATTTGT TCAACAAGTC ACTAGAAATT GAACCTCGAA
3121ACTGCAAACA GCCACCTCGA TTTCCTAGGA AATCAACTTT TTCCTTAAAA TCTCCTGGAA
3181TAAGGCCTAA CACAGGCCGA CATGGCTCTA CCTCAGGTAC TTTACGAGGT CACCCAACAC
3241CATTAGAAAG AGAACCATGT AAAATAAGCT TTAGTCGGAT TGCTGAAACT GAGCTGGAAT
3301CAACAGTGTC AGCACCAACC TCTCCAAATA CACCATCTAC TCCACCAGTA TCTGCTTCTT
3361CAGACCTTAG TGTATTTTTA GATGTGGATC TCAACAGCTC CTGTGGCAGC AATAGCATCT
3421TTGCTCCAGT GCTTTTGCCA CATTCAAAGT CTTTCTTTAG TTCATGTGGT AGTTTACATA
3481AACTAAGTGA AGAGCCCCTG ATTCCTCCTC CTCTTCCTCC TCGAAAAAAG TTTGATCATG
3541ATGCTTCAAA TTCCAAGGGA AATATGAAAT CTGATGATGA TCCTCCTGCT ATTCCACCGA
3601GACAGCCTCC TCCTCCAAAG GTAAAACCCA GAGTTCCTGT TCCTACTGGT GCATTTGATG
3661GGCCTCTGCA TAGTCCACCT CCGCCACCAC CAAGAGATCC TCTTCCTGAT ACCCCTCCAC
3721CAGTTCCCCT TCGGCCTCCA GAACACTTTA TAAACTGTCC ATTTAATCTT CAGCCACCTC
3781CACTGGGGCA TCTTCACAGA GATTCAGACT GGCTCAGAGA CATTAGTACG TGTCCAAATT
3841CGCCAAGCAC TCCTCCTAGC ACACCCTCTC CAAGGGTACC GCGTCGATGC TATGTGCTCA
3901GTTCTAGTCA GAATAATCTT GCTCATCCTC CAGCTCCCCC TGTTCCACCA AGGCAGAATT
3961CAAGCCCTCA TCTGCCAAAA CTGCCACCAA AGACTTACAA ACGGGAGCTT TCGCACCCCC
4021CATTGTACAG ACTGCCTTTG CTAGAAAATG CAGAAACTCC CCAATTGCCA ACTTTCTTGT
4081ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)