Construct: ORF ccsbBroadEn_01693

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012334.1_s300c1, BRDN0000386107
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TNNT2 (7139)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7139 TNNT2 troponin T2, cardiac type NM_001001431.3 100% 100%
2 human 7139 TNNT2 troponin T2, cardiac type XM_017002217.1 100% 100%
3 human 7139 TNNT2 troponin T2, cardiac type XM_017002216.2 99.3% 99.3% 569_574delAGGCCC
4 human 7139 TNNT2 troponin T2, cardiac type XM_024449455.1 99.3% 99.3% 569_574delAGGCCC
5 human 7139 TNNT2 troponin T2, cardiac type NM_001001430.3 98.9% 98.9% 569_577delAGCAGGCCC
6 human 7139 TNNT2 troponin T2, cardiac type NM_001276347.2 98.9% 98.9% 569_577delAGCAGGCCC
7 human 7139 TNNT2 troponin T2, cardiac type XM_006711508.3 98.9% 98.9% 569_577delAGCAGGCCC
8 human 7139 TNNT2 troponin T2, cardiac type XM_006711509.3 98.6% 98.6% 128_129insAGA;566_574delAGCAGGCCC
9 human 7139 TNNT2 troponin T2, cardiac type XM_024449454.1 98.6% 98.6% 128_129insAGA;566_574delAGCAGGCCC
10 human 7139 TNNT2 troponin T2, cardiac type NM_001001432.3 97.5% 97.5% 48_49insGAAGAAGCAGCTGTT;554_559delAGGCCC
11 human 7139 TNNT2 troponin T2, cardiac type XM_011509942.2 97.2% 97.2% 48_49insGAAGAAGCAGCTGTT;554_562delAGCAGGCCC
12 human 7139 TNNT2 troponin T2, cardiac type XM_011509943.2 97.2% 97.2% 48_49insGAAGAAGCAGCTGTT;554_562delAGCAGGCCC
13 human 7139 TNNT2 troponin T2, cardiac type XM_011509944.2 96.8% 96.8% 48_49insGAAGAAGCAGCTGTT;113_114insAGA;551_559delAGCAGGCCC
14 human 7139 TNNT2 troponin T2, cardiac type NM_000364.4 96.6% 96.6% 67_96del
15 human 7139 TNNT2 troponin T2, cardiac type XM_011509940.2 95.9% 95.9% 67_96del;599_604delAGGCCC
16 human 7139 TNNT2 troponin T2, cardiac type NM_001276345.2 95.6% 95.6% 67_96del;599_607delAGCAGGCCC
17 human 7139 TNNT2 troponin T2, cardiac type XM_011509938.2 95.6% 95.6% 67_96del;599_607delAGCAGGCCC
18 human 7139 TNNT2 troponin T2, cardiac type XM_024449450.1 95.6% 95.6% 67_96del;599_607delAGCAGGCCC
19 human 7139 TNNT2 troponin T2, cardiac type XM_011509941.2 95.6% 95.6% 67_96del;158_159insAGA;596_601delAGGCCC
20 human 7139 TNNT2 troponin T2, cardiac type XM_011509939.1 95.3% 95.3% 67_96del;158_159insAGA;596_604delAGCAGGCCC
21 human 7139 TNNT2 troponin T2, cardiac type NM_001276346.2 83% 83% 67_96del;158_159insAGA;291_292ins117
22 human 7139 TNNT2 troponin T2, cardiac type XM_011509946.1 78.4% 78.4% 0_1ins177;392_400delAGCAGGCCC
23 mouse 21956 Tnnt2 troponin T2, cardiac NM_001130178.2 83.9% 86.5% (many diffs)
24 mouse 21956 Tnnt2 troponin T2, cardiac NM_001130177.2 83.6% 85.5% (many diffs)
25 mouse 21956 Tnnt2 troponin T2, cardiac NM_011619.3 83.4% 88.5% (many diffs)
26 mouse 21956 Tnnt2 troponin T2, cardiac NM_001130176.1 82.8% 84.7% (many diffs)
27 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529393.3 82.8% 84.7% (many diffs)
28 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529394.3 82.8% 84.7% (many diffs)
29 mouse 21956 Tnnt2 troponin T2, cardiac NM_001130180.2 82.6% 87.6% (many diffs)
30 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529395.3 82.6% 87.6% (many diffs)
31 mouse 21956 Tnnt2 troponin T2, cardiac NM_001130174.2 82% 84.4% (many diffs)
32 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529391.3 81.2% 83.6% (many diffs)
33 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529392.3 81.2% 83.6% (many diffs)
34 mouse 21956 Tnnt2 troponin T2, cardiac NM_001130179.2 76.7% 79% (many diffs)
35 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529388.3 76.5% 78.2% (many diffs)
36 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529390.3 76.2% 80.6% (many diffs)
37 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529385.3 75.8% 77.5% (many diffs)
38 mouse 21956 Tnnt2 troponin T2, cardiac NM_001130181.2 75.5% 79.9% (many diffs)
39 mouse 21956 Tnnt2 troponin T2, cardiac NM_001130175.2 75.1% 77.2% (many diffs)
40 mouse 21956 Tnnt2 troponin T2, cardiac XM_006529383.3 74.4% 76.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
921
ORF length:
855
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAGAAG
61TTGGCATGTC TGACATAGAA GAGGTGGTGG AAGAGTACGA GGAGGAGGAG CAGGAAGAAG
121CAGCTGTTGA AGAGCAGGAG GAGGCAGCGG AAGAGGATGC TGAAGCAGAG GCTGAGACCG
181AGGAGACCAG GGCAGAAGAA GATGAAGAAG AAGAGGAAGC AAAGGAGGCT GAAGATGGCC
241CAATGGAGGA GTCCAAACCA AAGCCCAGGT CGTTCATGCC CAACTTGGTG CCTCCCAAGA
301TCCCCGATGG AGAGAGAGTG GACTTTGATG ACATCCACCG GAAGCGCATG GAGAAGGACC
361TGAATGAGTT GCAGGCGCTG ATCGAGGCTC ACTTTGAGAA CAGGAAGAAA GAGGAGGAGG
421AGCTCGTTTC TCTCAAAGAC AGGATCGAGA GACGTCGGGC AGAGCGGGCC GAGCAGCAGC
481GCATCCGGAA TGAGCGGGAG AAGGAGCGGC AGAACCGCCT GGCTGAAGAG AGGGCTCGAC
541GAGAGGAGGA GGAGAACAGG AGGAAGGCTG AGGATGAGGC CCGGAAGAAG AAGGCTTTGT
601CCAACATGAT GCATTTTGGG GGTTACATCC AGAAGACAGA GCGGAAAAGT GGGAAGAGGC
661AGACTGAGCG GGAAAAGAAG AAGAAGATTC TGGCTGAGAG GAGGAAGGTG CTGGCCATTG
721ACCACCTGAA TGAAGATCAG CTGAGGGAGA AGGCCAAGGA GCTGTGGCAG AGCATCTATA
781ACTTGGAGGC AGAGAAGTTC GACCTGCAGG AGAAGTTCAA GCAGCAGAAA TATGAGATCA
841ATGTTCTCCG AAACAGGATC AACGATAACC AGAAAGTCTC CAAGACCCGC GGGAAGGCTA
901AAGTCACCGG GCGCTGGAAA TACCCAACTT TCTTGTACAA AGTtggcatt ataagaaagc
961attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)