Construct: ORF ccsbBroadEn_01694
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009516.1_s300c1, BRDN0000391591
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNNT3 (7140)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_001042782.3 | 100% | 100% | |
| 2 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_001297646.2 | 100% | 100% | |
| 3 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_001367844.1 | 100% | 100% | |
| 4 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_017018211.1 | 98.6% | 98% | (many diffs) |
| 5 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_006718299.2 | 98.6% | 98% | 60_61insGAGGAA;72T>A;75_77delACC |
| 6 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_024448671.1 | 98.6% | 98% | 60_61insGAGGAA;72T>A;75_77delACC |
| 7 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_017018210.1 | 98.4% | 98.4% | 66_77del |
| 8 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_006718300.3 | 98% | 98% | 63_64insGAAGAGGAAGTTCAA |
| 9 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_017018212.1 | 97.8% | 97.2% | (many diffs) |
| 10 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_001367845.1 | 97.7% | 97.2% | (many diffs) |
| 11 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_001042781.3 | 97.5% | 97.2% | 67_81del;93T>A;96_98delACC |
| 12 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_001367842.1 | 97.5% | 97.2% | 67_81del;93T>A;96_98delACC |
| 13 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_024448669.1 | 97.5% | 97.2% | 67_81del;93T>A;96_98delACC |
| 14 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_001367848.1 | 97.2% | 97.2% | 61_81del |
| 15 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_017018209.1 | 97.2% | 97.2% | 61_81del |
| 16 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_001042780.3 | 96.9% | 96% | (many diffs) |
| 17 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_006757.4 | 96.8% | 96.8% | 79_102del |
| 18 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_017018213.1 | 96.3% | 96.4% | (many diffs) |
| 19 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_017018215.1 | 96.2% | 95.6% | (many diffs) |
| 20 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_024448672.1 | 96.2% | 95.6% | (many diffs) |
| 21 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_001363561.2 | 95.7% | 95.7% | 66_98del |
| 22 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_001367847.1 | 95.7% | 95.7% | 66_98del |
| 23 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_001367850.1 | 95.6% | 95.6% | 43_44ins33 |
| 24 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_017018219.1 | 95.6% | 95.6% | 30_31insGAGCAGTACGAAGAAGAA;45_46insGAAGAGGAAGTTCAA |
| 25 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_001367849.1 | 95.5% | 95.2% | (many diffs) |
| 26 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_017018208.1 | 95.4% | 95.4% | 66_77del;91_114del |
| 27 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_024448670.1 | 95.1% | 96% | (many diffs) |
| 28 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_017018214.1 | 94.9% | 94% | (many diffs) |
| 29 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_017018218.1 | 94.5% | 96.4% | 30_31insGAGCAGTACGAAGAAGAA;61_84del |
| 30 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_001367843.1 | 94.5% | 93.3% | (many diffs) |
| 31 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_017018205.1 | 94.5% | 93.3% | (many diffs) |
| 32 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_017018207.1 | 94.3% | 94.3% | 61_81del;100_123del |
| 33 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_017018216.1 | 93.9% | 92.7% | (many diffs) |
| 34 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_017018217.1 | 93.1% | 94.9% | 30_31insGAGCAGTACGAAGAAGAA;48_59del;73_96del |
| 35 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_001367846.1 | 92.9% | 92.9% | 66_98del;112_135del |
| 36 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_011520343.2 | 92.9% | 92.9% | 66_98del;112_135del |
| 37 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_017018206.1 | 92.9% | 92.9% | 66_98del;112_135del |
| 38 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_006718294.3 | 92.9% | 91.9% | (many diffs) |
| 39 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | XM_006718288.3 | 87.4% | 82.6% | (many diffs) |
| 40 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_001367851.1 | 76% | 76% | 0_1ins180 |
| 41 | human | 7140 | TNNT3 | troponin T3, fast skeletal ... | NM_001367852.1 | 76% | 76% | 0_1ins180 |
| 42 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | NM_001347548.1 | 88.9% | 96% | (many diffs) |
| 43 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | NM_001163668.1 | 87.3% | 94.1% | (many diffs) |
| 44 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508588.1 | 87.1% | 93.7% | (many diffs) |
| 45 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508590.1 | 87% | 92.8% | (many diffs) |
| 46 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | NM_001163670.1 | 86.9% | 93.6% | (many diffs) |
| 47 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | NM_011620.3 | 86.6% | 91.6% | (many diffs) |
| 48 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | NM_001347549.1 | 86.4% | 92.4% | (many diffs) |
| 49 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | NM_001163669.1 | 85.8% | 92.6% | (many diffs) |
| 50 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508585.1 | 85.6% | 92.2% | (many diffs) |
| 51 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | NM_001347551.1 | 85% | 91.6% | (many diffs) |
| 52 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508587.1 | 84.9% | 90.5% | (many diffs) |
| 53 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | NM_001347547.1 | 84.7% | 90.1% | (many diffs) |
| 54 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508591.1 | 84.5% | 89.3% | (many diffs) |
| 55 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | NM_001163666.1 | 84.5% | 91.2% | (many diffs) |
| 56 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | NM_001347550.1 | 84.1% | 88% | (many diffs) |
| 57 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | NM_001347546.1 | 84% | 89.3% | (many diffs) |
| 58 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508584.1 | 83.4% | 89.1% | (many diffs) |
| 59 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508586.1 | 83.3% | 87.1% | (many diffs) |
| 60 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | NM_001163667.1 | 83% | 89.5% | (many diffs) |
| 61 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | NM_001347552.1 | 82.6% | 88% | (many diffs) |
| 62 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | NM_001163664.1 | 81.7% | 88.2% | (many diffs) |
| 63 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_017322121.1 | 81% | 82.8% | (many diffs) |
| 64 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | NM_001163665.1 | 79.5% | 84.9% | (many diffs) |
| 65 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_017322119.1 | 78.7% | 81.9% | (many diffs) |
| 66 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_017322120.1 | 76.5% | 78.7% | (many diffs) |
| 67 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508567.3 | 56.1% | 57.6% | (many diffs) |
| 68 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508563.3 | 55.5% | 56.9% | (many diffs) |
| 69 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508561.3 | 55.3% | 56.5% | (many diffs) |
| 70 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508562.3 | 55.3% | 56.5% | (many diffs) |
| 71 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508569.3 | 55.3% | 56.2% | (many diffs) |
| 72 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508570.3 | 55.3% | 56.2% | (many diffs) |
| 73 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508573.3 | 55.2% | 56% | (many diffs) |
| 74 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508553.3 | 54.8% | 56.2% | (many diffs) |
| 75 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508575.3 | 54.8% | 56% | (many diffs) |
| 76 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508571.3 | 54.7% | 54.6% | (many diffs) |
| 77 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508572.3 | 54.7% | 54.6% | (many diffs) |
| 78 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508557.3 | 54.6% | 55.8% | (many diffs) |
| 79 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508558.3 | 54.6% | 54.7% | (many diffs) |
| 80 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508576.3 | 54.1% | 54.6% | (many diffs) |
| 81 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508577.3 | 54.1% | 54.6% | (many diffs) |
| 82 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508550.3 | 54.1% | 55.1% | (many diffs) |
| 83 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508551.3 | 53.9% | 54.3% | (many diffs) |
| 84 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508543.3 | 53.6% | 54.9% | (many diffs) |
| 85 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508546.3 | 53.5% | 54.5% | (many diffs) |
| 86 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508547.3 | 53.3% | 53.9% | (many diffs) |
| 87 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_006508542.3 | 53% | 54.4% | (many diffs) |
| 88 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_017322117.1 | 53% | 51.6% | (many diffs) |
| 89 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_017322118.1 | 52.1% | 50.3% | (many diffs) |
| 90 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_017322116.1 | 51.9% | 50.6% | (many diffs) |
| 91 | mouse | 21957 | Tnnt3 | troponin T3, skeletal, fast | XM_017322115.1 | 51.3% | 50.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 819
- ORF length:
- 750
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GTCTGACGAG GAAGTTGAAC AGGTGGAGGA GCAGTACGAA GAAGAAGAGG 121 AAGCCCAGGA GGAAGAGGAA GTTCAAGAAG AGGAGAAACC GAGACCCAAA CTCACTGCTC 181 CTAAGATCCC AGAAGGGGAG AAAGTGGACT TCGATGACAT CCAGAAGAAG CGTCAGAACA 241 AAGACCTAAT GGAGCTCCAG GCCCTCATCG ACAGCCACTT TGAAGCCCGG AAGAAGGAGG 301 AGGAGGAGCT GGTCGCTCTC AAAGAGAGAA TCGAGAAGCG CCGTGCAGAG AGAGCGGAGC 361 AGCAGAGGAT TCGTGCAGAG AAGGAGAGGG AGCGCCAGAA CAGACTGGCG GAGGAAAAGG 421 CCAGAAGGGA GGAGGAGGAT GCCAAGAGGA GGGCAGAGGA CGACCTGAAG AAGAAGAAAG 481 CTCTGTCTTC CATGGGAGCC AACTACAGCA GCTACCTGGC CAAGGCTGAC CAGAAGAGAG 541 GCAAGAAGCA GACAGCCCGG GAAATGAAGA AGAAGATTCT GGCTGAGAGA CGCAAGCCGC 601 TCAACATCGA TCACCTTGGT GAAGACAAAC TGAGGGACAA GGCCAAGGAG CTCTGGGAGA 661 CCCTGCACCA GCTGGAGATT GACAAGTTCG AGTTTGGGGA GAAGCTGAAA CGCCAGAAAT 721 ATGACATCAC CACGCTCAGG AGCCGCATTG ACCAGGCCCA GAAGCACAGC AAGAAGGCTG 781 GGACCCCAGC CAAGGGCAAA GTCGGCGGGC GCTGGAAGTT GCCAACTTTC TTGTACAAAG 841 Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac