Construct: ORF ccsbBroadEn_01960

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005583.1_s300c1, BRDN0000394438
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CASK (8573)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8573 CASK calcium/calmodulin dependen... NM_001126054.2 100% 100%
2 human 8573 CASK calcium/calmodulin dependen... NM_001126055.2 98.7% 98.7% 1015_1016insGACTTCTAGCAGCAGAAA;2067_2081del
3 human 8573 CASK calcium/calmodulin dependen... XM_011543995.2 98.1% 98% 173_190del;1033_1034insGACTTCTAGCAGCAGAAA;2085_2099del
4 human 8573 CASK calcium/calmodulin dependen... NM_003688.3 97.5% 97.5% 1737_1805del
5 human 8573 CASK calcium/calmodulin dependen... XM_006724566.3 97.4% 97.4% 1015_1016insGACTTCTAGCAGCAGAAA;1717_1718ins36;2031_2045del
6 human 8573 CASK calcium/calmodulin dependen... NM_001367721.1 96.9% 96.9% 1737_1805del;2154_2168del
7 human 8573 CASK calcium/calmodulin dependen... XM_011543996.2 96.8% 96.6% (many diffs)
8 human 8573 CASK calcium/calmodulin dependen... XM_011543994.2 96.5% 95.9% (many diffs)
9 human 8573 CASK calcium/calmodulin dependen... XM_005272686.4 96.3% 96.3% 1015_1016insGACTTCTAGCAGCAGAAA;1719_1787del;2136_2150del
10 human 8573 CASK calcium/calmodulin dependen... XM_011543993.2 95.7% 95.6% (many diffs)
11 human 8573 CASK calcium/calmodulin dependen... XM_024452473.1 76.9% 76.9% (many diffs)
12 human 8573 CASK calcium/calmodulin dependen... XM_011543997.3 76.3% 76.3% (many diffs)
13 mouse 12361 Cask calcium/calmodulin-dependen... XM_006527561.3 95.1% 99.8% (many diffs)
14 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318370.1 94.5% 99.1% (many diffs)
15 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318369.1 93.9% 98.5% (many diffs)
16 mouse 12361 Cask calcium/calmodulin-dependen... NM_009806.3 93.9% 98.6% (many diffs)
17 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247440.2 93.5% 97.7% (many diffs)
18 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247441.2 93.3% 97.9% (many diffs)
19 mouse 12361 Cask calcium/calmodulin-dependen... NM_001284504.1 93.1% 97.8% (many diffs)
20 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318368.1 92.9% 97% (many diffs)
21 mouse 12361 Cask calcium/calmodulin-dependen... XM_006527560.3 92.7% 97.3% (many diffs)
22 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318371.1 92.7% 97.2% (many diffs)
23 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247439.2 92.4% 96.5% (many diffs)
24 mouse 12361 Cask calcium/calmodulin-dependen... NM_001284503.1 92.2% 96.8% (many diffs)
25 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318367.1 92.1% 96.6% (many diffs)
26 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247438.2 91.6% 96.1% (many diffs)
27 mouse 12361 Cask calcium/calmodulin-dependen... NM_001284505.1 91.6% 96.2% (many diffs)
28 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247436.2 90% 94.4% (many diffs)
29 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318366.1 89.3% 93.7% (many diffs)
30 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247437.1 88.7% 93.1% (many diffs)
31 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247435.2 88.6% 92.5% (many diffs)
32 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247433.1 88.4% 92.8% (many diffs)
33 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247432.1 88.3% 92.6% (many diffs)
34 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247431.1 87.8% 92.1% (many diffs)
35 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247434.1 87.2% 91.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2763
ORF length:
2694
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACACAAAAG
61TTGGCACCAT GGCCGACGAC GACGTGCTGT TCGAGGATGT GTACGAGCTG TGCGAGGTGA
121TCGGAAAGGG TCCCTTCAGT GTTGTACGAC GATGTATCAA CAGAGAAACT GGGCAACAAT
181TTGCTGTAAA AATTGTTGAT GTAGCCAAGT TCACATCAAG TCCAGGGTTA AGTACAGAAG
241ATCTAAAGCG GGAAGCCAGT ATCTGTCATA TGCTGAAACA TCCACACATT GTAGAGTTAT
301TGGAGACATA TAGCTCAGAT GGAATGCTTT ACATGGTTTT CGAATTTATG GATGGAGCAG
361ATCTGTGTTT TGAAATCGTA AAGCGAGCTG ACGCTGGTTT TGTGTACAGT GAAGCTGTAG
421CCAGCCATTA TATGAGACAG ATACTGGAAG CTCTACGCTA CTGCCATGAT AATAACATAA
481TTCACAGGGA TGTGAAGCCC CACTGTGTTC TCCTTGCCTC AAAAGAAAAC TCGGCACCTG
541TTAAACTTGG AGGCTTTGGG GTAGCTATTC AATTAGGGGA GTCTGGACTT GTAGCTGGAG
601GACGTGTTGG AACACCTCAT TTTATGGCAC CAGAAGTGGT CAAAAGAGAG CCTTACGGAA
661AGCCTGTAGA CGTCTGGGGG TGCGGTGTGA TCCTTTTTAT CCTGCTCAGT GGTTGTTTGC
721CTTTTTACGG AACCAAGGAA AGATTGTTTG AAGGCATTAT TAAAGGAAAA TATAAGATGA
781ATCCAAGGCA GTGGAGCCAT ATCTCTGAAA GTGCCAAAGA CCTAGTACGT CGCATGCTGA
841TGCTGGATCC AGCTGAAAGG ATCACTGTTT ATGAAGCACT GAATCACCCA TGGCTTAAGG
901AGCGGGATCG TTACGCCTAC AAGATTCATC TTCCAGAAAC AGTAGAGCAG CTGAGGAAAT
961TCAATGCAAG GAGGAAACTA AAGGGTGCAG TACTAGCCGC TGTGTCAAGT CACAAATTCA
1021ACTCATTCTA TGGGGATCCC CCTGAAGAGT TACCAGATTT CTCCGAAGAC CCTACCTCCT
1081CAGGACTTCT AGCAGCAGAA AGAGCAGTCT CACAGGTGCT GGACAGCCTG GAAGAGATTC
1141ATGCGCTTAC AGACTGCAGT GAAAAGGACC TAGATTTTCT ACACAGTGTT TTCCAGGATC
1201AGCATCTTCA CACACTACTA GATCTGTATG ACAAAATTAA CACAAAGTCT TCACCACAAA
1261TCAGGAATCC TCCAAGCGAT GCAGTACAGA GAGCCAAAGA GGTATTGGAA GAAATTTCAT
1321GTTACCCTGA GAATAACGAC GCAAAGGAAC TAAAGCGTAT TTTAACACAA CCTCATTTCA
1381TGGCCTTACT TCAGACTCAC GACGTAGTGG CACATGAAGT TTACAGTGAT GAAGCATTGA
1441GGGTCACACC TCCTCCCACC TCTCCCTATT TAAACGGCGA TTCTCCAGAA AGTGCTAACG
1501GAGACATGGA TATGGAGAAT GTGACCAGAG TTCGGCTGGT ACAGTTTCAA AAGAACACAG
1561ATGAACCAAT GGGAATCACT TTAAAAATGA ATGAACTAAA TCATTGTATT GTTGCAAGAA
1621TTATGCATGG GGGCATGATT CACAGGCAAG GTACACTTCA TGTTGGTGAT GAAATTCGAG
1681AAATCAATGG CATCAGTGTG GCTAACCAAA CAGTGGAACA ACTGCAAAAA ATGCTTAGGG
1741AAATGCGGGG GAGTATTACC TTCAAGATTG TGCCAAGTTA CCGCACTCAG TCTTCGTCCT
1801GTGAGGACTT GCCATCAACT ACCCAACCAA AAGGACGACA GATCTATGTA AGAGCACAAT
1861TTGAATATGA TCCAGCCAAG GATGACCTCA TCCCCTGTAA AGAAGCTGGC ATTCGATTCA
1921GAGTTGGTGA CATCATCCAG ATTATTAGTA AGGATGATCA TAATTGGTGG CAGGGTAAAC
1981TGGAAAACTC CAAAAATGGA ACTGCAGGTC TCATTCCTTC TCCTGAACTT CAGGAATGGC
2041GAGTAGCTTG CATTGCCATG GAGAAGACCA AACAGGAGCA GCAGGCCAGC TGTACTTGGT
2101TTGGCAAGAA AAAGAAGCAG TACAAAGATA AATATTTGGC AAAGCACAAT GCAGATCTTG
2161TCACATATGA AGAAGTAGTA AAACTGCCAG CATTCAAGAG GAAAACACTA GTCTTATTAG
2221GCGCACATGG TGTTGGGAGA AGACACATAA AAAACACTCT CATCACAAAG CACCCAGACC
2281GGTTTGCGTA CCCTATTCCA CATACAACCA GACCTCCAAA GAAAGACGAA GAAAATGGAA
2341AGAATTATTA CTTTGTATCT CATGACCAAA TGATGCAAGA CATCTCTAAT AACGAGTACT
2401TGGAGTACGG CAGCCACGAG GATGCGATGT ATGGGACAAA ACTGGAGACC ATCCGGAAGA
2461TCCACGAGCA GGGGCTGATT GCAATACTGG ACGTGGAGCC TCAGGCACTG AAGGTCCTGA
2521GAACTGCAGA GTTTGCTCCT TTTGTTGTTT TCATTGCTGC ACCAACTATT ACTCCAGGTT
2581TAAATGAGGA TGAATCTCTT CAGCGTCTGC AGAAGGAGTC TGACATCTTA CAGAGAACAT
2641ATGCACACTA CTTCGATCTC ACAATTATCA ACAATGAAAT TGATGAGACA ATCAGACATC
2701TGGAGGAAGC TGTTGAGCTC GTGTGCACAG CCCCACAGTG GGTCCCTGTC TCCTGGGTCT
2761ATTTGCCAAC TTTCTTGTAC AAAGTtggca ttataagaaa gcattgctta tcaatttgtt
2821gcaacgaac

Download FASTA (ORF) (Full)