Construct: ORF ccsbBroadEn_01987
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003641.1_s300c1, BRDN0000396144
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VAMP4 (8674)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8674 | VAMP4 | vesicle associated membrane... | NM_003762.5 | 100% | 100% | |
| 2 | human | 8674 | VAMP4 | vesicle associated membrane... | NM_001185127.2 | 99.2% | 99.2% | 113_114insAAG |
| 3 | human | 8674 | VAMP4 | vesicle associated membrane... | XM_017002713.2 | 70.9% | 52.7% | 217_218ins46;300_301ins77 |
| 4 | human | 8674 | VAMP4 | vesicle associated membrane... | NR_033704.2 | 15.8% | 1_205del;629_2675del | |
| 5 | mouse | 53330 | Vamp4 | vesicle-associated membrane... | NM_001347125.1 | 93.8% | 97.1% | (many diffs) |
| 6 | mouse | 53330 | Vamp4 | vesicle-associated membrane... | XM_006496934.2 | 93.8% | 97.1% | (many diffs) |
| 7 | mouse | 53330 | Vamp4 | vesicle-associated membrane... | NM_016796.3 | 89.7% | 92.9% | (many diffs) |
| 8 | mouse | 53330 | Vamp4 | vesicle-associated membrane... | XM_006496936.3 | 89.7% | 92.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 489
- ORF length:
- 423
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCC TCCCAAGTTT AAGCGCCACC TCAATGATGA TGATGTCACA GGTTCTGTGA 121 AAAGTGAAAG GAGAAATCTT TTGGAAGATG ATTCAGATGA AGAAGAGGAC TTTTTTCTAA 181 GGGGACCATC TGGACCAAGA TTTGGACCTA GAAATGATAA AATTAAGCAT GTTCAGAATC 241 AAGTGGATGA AGTTATTGAT GTCATGCAAG AAAATATTAC AAAGGTAATT GAGAGAGGGG 301 AGAGACTAGA TGAACTACAG GACAAATCAG AAAGCTTATC GGATAATGCA ACAGCTTTTA 361 GCAACAGATC CAAACAACTT CGAAGGCAAA TGTGGTGGCG TGGATGCAAA ATAAAAGCCA 421 TCATGGCTTT GGTTGCTGCT ATCCTTTTGC TAGTGATTAT CATTCTTATA GTCATGAAAT 481 ACCGTACTTG CCCGACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa 541 tttgttgcaa cgaac