Transcript: Human NM_003762.5

Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
VAMP4 (8674)
Length:
4977
CDS:
206..631

Additional Resources:

NCBI RefSeq record:
NM_003762.5
NBCI Gene record:
VAMP4 (8674)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_003762.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000129433 GACCATCTGGACCAAGATTTG pLKO.1 324 CDS 100% 10.800 15.120 N VAMP4 n/a
2 TRCN0000130415 GACCAAGATTTGGACCTAGAA pLKO.1 333 CDS 100% 4.950 3.960 N VAMP4 n/a
3 TRCN0000098991 CCAAGATTTGGACCTAGAAAT pLKO.1 335 CDS 100% 13.200 9.240 N Vamp4 n/a
4 TRCN0000348352 GGACCATCTGGACCAAGATTT pLKO_005 323 CDS 100% 13.200 9.240 N Vamp4 n/a
5 TRCN0000381837 GGACCATCTGGACCAAGATTT pLKO_005 323 CDS 100% 13.200 9.240 N VAMP4 n/a
6 TRCN0000348275 TCAGAAAGCTTATCGGATAAT pLKO_005 467 CDS 100% 13.200 9.240 N Vamp4 n/a
7 TRCN0000382053 TCAGAAAGCTTATCGGATAAT pLKO_005 467 CDS 100% 13.200 9.240 N VAMP4 n/a
8 TRCN0000098994 CAGGACAAATCAGAAAGCTTA pLKO.1 458 CDS 100% 4.950 3.465 N Vamp4 n/a
9 TRCN0000128865 GCAAAGAGAACTAGCCTCTAT pLKO.1 839 3UTR 100% 4.950 3.465 N VAMP4 n/a
10 TRCN0000382334 GTCATGAAATACCGTACTTGA pLKO_005 611 CDS 100% 4.950 3.465 N VAMP4 n/a
11 TRCN0000129282 CTGGACCAAGATTTGGACCTA pLKO.1 330 CDS 100% 2.640 1.848 N VAMP4 n/a
12 TRCN0000381489 CAAACAACTTCGAAGGCAAAT pLKO_005 511 CDS 100% 10.800 6.480 N VAMP4 n/a
13 TRCN0000129123 GCATGTTCAGAATCAAGTGGA pLKO.1 367 CDS 100% 2.640 1.584 N VAMP4 n/a
14 TRCN0000166364 CACACACACACACACACACAA pLKO.1 1720 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_003762.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01987 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01987 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000481388 CTGACATGCAGATAACTTGGTTAC pLX_317 100% 100% 100% V5 n/a
4 ccsbBroadEn_15641 pDONR223 0% 99.2% 99.2% None 114_116delAAG n/a
5 ccsbBroad304_15641 pLX_304 0% 99.2% 99.2% V5 114_116delAAG n/a
6 TRCN0000467223 TCCCTGGAGCACACAACACCTAAC pLX_317 80.6% 99% 98.5% V5 114_116delAAG;416A>C n/a
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