Construct: ORF ccsbBroadEn_02020
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011751.1_s300c1, BRDN0000456916
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALR2 (8811)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8811 | GALR2 | galanin receptor 2 | NM_003857.4 | 100% | 100% | |
| 2 | human | 8811 | GALR2 | galanin receptor 2 | XM_011525427.3 | 79.5% | 74.3% | (many diffs) |
| 3 | mouse | 14428 | Galr2 | galanin receptor 2 | NM_010254.4 | 81.2% | 80.9% | (many diffs) |
| 4 | mouse | 14428 | Galr2 | galanin receptor 2 | XM_011248727.2 | 80.3% | 80.1% | (many diffs) |
| 5 | mouse | 14428 | Galr2 | galanin receptor 2 | XM_011248728.2 | 80.2% | 80.1% | (many diffs) |
| 6 | mouse | 14428 | Galr2 | galanin receptor 2 | XM_011248729.2 | 76.9% | 78.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1230
- ORF length:
- 1161
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GAACGTCTCG GGCTGCCCAG GGGCCGGGAA CGCGAGCCAG GCGGGCGGCG 121 GGGGAGGCTG GCACCCCGAG GCGGTCATCG TGCCCCTGCT CTTCGCGCTC ATCTTCCTCG 181 TGGGCACCGT GGGCAACACG CTGGTGCTGG CGGTGCTGCT GCGCGGCGGC CAGGCGGTCA 241 GCACTACCAA CCTGTTCATC CTTAACCTGG GCGTGGCCGA CCTGTGTTTC ATCCTGTGCT 301 GCGTGCCCTT CCAGGCCACC ATCTACACCC TGGACGGCTG GGTGTTCGGC TCGCTGCTGT 361 GCAAGGCGGT GCACTTCCTC ATCTTCCTCA CCATGCACGC CAGCAGCTTC ACGCTGGCCG 421 CCGTCTCCCT GGACAGGTAT CTGGCCATCC GCTACCCGCT GCACTCCCGC GAGCTGCGCA 481 CGCCTCGAAA CGCGCTGGCA GCCATCGGGC TCATCTGGGG GCTGTCGCTG CTCTTCTCCG 541 GGCCCTACCT GAGCTACTAC CGCCAGTCGC AGCTGGCCAA CCTGACCGTG TGCCATCCCG 601 CGTGGAGCGC CCCTCGCCGC CGCGCCATGG ACATCTGCAC CTTCGTCTTC AGCTACCTGC 661 TTCCTGTGCT GGTTCTCGGC CTGACCTACG CGCGCACCTT GCGCTACCTC TGGCGCGCCG 721 TCGACCCGGT GGCCGCGGGC TCGGGTGCCC GGCGCGCCAA GCGCAAGGTG ACACGCATGA 781 TCCTCATCGT GGCCGCGCTC TTCTGCCTCT GCTGGATGCC CCACCACGCG CTCATCCTCT 841 GCGTGTGGTT CGGCCAGTTC CCGCTCACGC GCGCCACTTA TGCGCTTCGC ATCCTCTCGC 901 ACCTGGTCTC CTACGCCAAC TCCTGCGTCA ACCCCATCGT TTACGCGCTG GTCTCCAAGC 961 ACTTCCGCAA AGGCTTCCGC ACGATCTGCG CGGGCCTGCT GGGCCGTGCC CCAGGCCGAG 1021 CCTCGGGCCG TGTGTGCGCT GCCGCGCGGG GCACCCACAG TGGCAGCGTG TTGGAGCGCG 1081 AGTCCAGCGA CCTGTTGCAC ATGAGCGAGG CGGCGGGGGC CCTTCGTCCC TGCCCCGGCG 1141 CTTCCCAGCC ATGCATCCTC GAGCCCTGTC CTGGCCCGTC CTGGCAGGGC CCAAAGGCAG 1201 GCGACAGCAT CCTGACGGTT GATGTGGCCT TGCCAACTTT CTTTACAAAG TTGGcattat 1261 aagaaagcat tgcttatcaa tttgttgcaa cgaac