Transcript: Mouse NM_010254.4

Mus musculus galanin receptor 2 (Galr2), mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Galr2 (14428)
Length:
1957
CDS:
548..1663

Additional Resources:

NCBI RefSeq record:
NM_010254.4
NBCI Gene record:
Galr2 (14428)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_010254.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000027993 CACGAACCTATTCATCCTCAA pLKO.1 721 CDS 100% 4.050 5.670 N Galr2 n/a
2 TRCN0000027990 GCATCCTTTCACATCTAGTAT pLKO.1 1365 CDS 100% 5.625 3.938 N Galr2 n/a
3 TRCN0000028018 CCAACTGCACAACCTTGAGTA pLKO.1 1620 CDS 100% 4.950 3.465 N Galr2 n/a
4 TRCN0000027964 CCATCTATACCCTGGACGATT pLKO.1 795 CDS 100% 4.950 3.465 N Galr2 n/a
5 TRCN0000028017 CCAAGCATTTCCGCAAAGGTT pLKO.1 1431 CDS 100% 3.000 2.100 N Galr2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_010254.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02020 pDONR223 100% 81.2% 80.9% None (many diffs) n/a
2 TRCN0000488646 CTCAATCCTGCACAAGAGCCAGTA pLX_317 26.6% 81.2% 80.9% V5 (many diffs) n/a
3 TRCN0000488270 CGACAACACACAGATGAATAACCT pLX_317 26.8% 81.2% 80.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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