Construct: ORF ccsbBroadEn_02210
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008282.1_s300c1, BRDN0000385007
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CARTPT (9607)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9607 | CARTPT | CART prepropeptide | NM_004291.4 | 100% | 100% | |
| 2 | mouse | 27220 | Cartpt | CART prepropeptide | NM_001081493.2 | 92.8% | 96.5% | (many diffs) |
| 3 | mouse | 27220 | Cartpt | CART prepropeptide | NM_013732.7 | 83.4% | 86.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 414
- ORF length:
- 348
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGA GAGCTCCCGC GTGAGGCTGC TGCCCCTCCT GGGCGCCGCC CTGCTGCTGA 121 TGCTACCTCT GTTGGGTACC CGTGCCCAGG AGGACGCCGA GCTCCAGCCC CGAGCCCTGG 181 ACATCTACTC TGCCGTGGAT GATGCCTCCC ACGAGAAGGA GCTGATCGAA GCGCTGCAAG 241 AAGTCTTGAA GAAGCTCAAG AGTAAACGTG TTCCCATCTA TGAGAAGAAG TATGGCCAAG 301 TCCCCATGTG TGACGCCGGT GAGCAGTGTG CAGTGAGGAA AGGGGCAAGG ATCGGGAAGC 361 TGTGTGACTG TCCCCGAGGA ACCTCCTGCA ATTCCTTCCT CCTGAAGTGC TTATGCCCAA 421 CTTTCTTGAA CAAAGTtggc attataagaa agcattgctt atcaatttgt tgcaacgaac