Construct: ORF ccsbBroadEn_02270

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017461.1_s300c1, BRDN0000390941
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FAM20B (9917)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9917 FAM20B FAM20B glycosaminoglycan xy... NM_001324310.1 98.7% 98.7% 1213_1227del
2 human 9917 FAM20B FAM20B glycosaminoglycan xy... NM_001324311.1 98.7% 98.7% 1213_1227del
3 human 9917 FAM20B FAM20B glycosaminoglycan xy... NM_014864.4 98.7% 98.7% 1213_1227del
4 human 9917 FAM20B FAM20B glycosaminoglycan xy... XM_017003001.2 93.5% 93.5% 377_445del;1282_1296del
5 human 9917 FAM20B FAM20B glycosaminoglycan xy... XM_017003002.1 93.5% 93.5% 377_445del;1282_1296del
6 human 9917 FAM20B FAM20B glycosaminoglycan xy... XR_002958229.1 70.8% (many diffs)
7 mouse 215015 Fam20b family with sequence simila... NM_145413.4 89.7% 96% (many diffs)
8 mouse 215015 Fam20b family with sequence simila... XM_006496736.2 89.7% 96% (many diffs)
9 mouse 215015 Fam20b family with sequence simila... XM_006496737.2 89.7% 96% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1278
ORF length:
1212
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA GCTAAAGCAG CGAGTCGTGC TGTTAGCAAT TCTCCTTGTC ATTTTTATCT
121TCACCAAAGT TTTCCTGATT GACAACTTAG ATACATCAGC TGCCAACCGG GAGGACCAGA
181GGGCCTTTCA CCGAATGATG ACTGGCTTGC GGGTGGAGCT GGCACCCAAG CTGGACCATA
241CCTTGCAGTC TCCCTGGGAG ATTGCAGCCC AGTGGGTGGT TCCCCGGGAA GTGTACCCTG
301AAGAGACACC AGAGCTGGGG GCAGTCATGC ATGCCATGGC CACCAAGAAA ATCATTAAAG
361CTGATGTGGG TTATAAAGGG ACACAGCTGA AAGCCTTACT GATACTTGAA GGAGGCCAGA
421AAGTTGTTTT CAAACCTAAG CGGTATAGCC GAGACCATGT GGTGGAAGGG GAACCGTATG
481CTGGTTATGA TAGACACAAT GCAGAGGTAG CAGCCTTTCA CTTGGACAGG ATTCTGGGTT
541TCCACCGAGC CCCCTTGGTA GTTGGCAGAT TTGTTAATCT TCGGACAGAG ATCAAACCTG
601TCGCCACAGA GCAGCTGTTG AGCACCTTCC TAACTGTAGG AAACAATACT TGTTTTTATG
661GGAAGTGCTA TTACTGCCGA GAAACAGAAC CAGCTTGTGC TGATGGAGAC ATAATGGAGG
721GATCTGTCAC ACTTTGGCTT CCAGATGTGT GGCCTCTGCA GAAGCACCGT CACCCATGGG
781GCAGGACTTA CCGAGAAGGC AAATTGGCCA GGTGGGAGTA TGATGAGAGC TACTGTGATG
841CTGTGAAGAA AACGTCCCCT TATGACTCTG GCCCGCGCCT CTTGGACATC ATTGACACAG
901CTGTCTTTGA TTACCTGATT GGCAATGCTG ACCGCCATCA CTATGAGAGC TTTCAAGATG
961ATGAAGGCGC TAGTATGCTC ATCCTTCTTG ATAATGCCAA AAGCTTTGGG AACCCCTCGC
1021TGGATGAAAG AAGCATTCTT GCCCCTCTCT ATCAGTGTTG CATCATTCGG GTGTCCACCT
1081GGAACAGACT GAACTACCTA AAGAATGGTG TGCTAAAGTC TGCCTTAAAA TCTGCCATGG
1141CCCATGACCC CATCTCCCCA GTGCTCTCTG ATCCTCATCT GGACGCCGTG GACCAGCGGC
1201TCCTGAGTGT CCTGGCCACC GTGAAGCAGT GCACCGACCA GTTTGGGATG GACACAGTAC
1261TGGTGGAAGA CAGGATGCCT CTCTCACACT TGTACCCAAC TTTCTTGTAC AAAGTtggca
1321ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)