Transcript: Human XM_017003002.1

PREDICTED: Homo sapiens FAM20B glycosaminoglycan xylosylkinase (FAM20B), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FAM20B (9917)
Length:
5885
CDS:
164..1462

Additional Resources:

NCBI RefSeq record:
XM_017003002.1
NBCI Gene record:
FAM20B (9917)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017003002.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000135038 CGTGTATTCTTTGACCAGTAA pLKO.1 5164 3UTR 100% 4.950 6.930 N FAM20B n/a
2 TRCN0000297418 CGTGTATTCTTTGACCAGTAA pLKO_005 5164 3UTR 100% 4.950 6.930 N FAM20B n/a
3 TRCN0000431604 ACAGCACAATCAATTCCAAAT pLKO_005 1509 3UTR 100% 10.800 7.560 N Fam20b n/a
4 TRCN0000138755 GAGCTACTGTGATGCTGTGAA pLKO.1 994 CDS 100% 4.950 3.465 N FAM20B n/a
5 TRCN0000137827 GATGCCTCTCTCACACTTGTA pLKO.1 1441 CDS 100% 4.950 3.465 N FAM20B n/a
6 TRCN0000278243 GATGCCTCTCTCACACTTGTA pLKO_005 1441 CDS 100% 4.950 3.465 N FAM20B n/a
7 TRCN0000138872 GCCTTTCACTTGGACAGGATT pLKO.1 680 CDS 100% 4.950 3.465 N FAM20B n/a
8 TRCN0000297420 GCCTTTCACTTGGACAGGATT pLKO_005 680 CDS 100% 4.950 3.465 N FAM20B n/a
9 TRCN0000134558 GCTGTCTTTGATTACCTGATT pLKO.1 1067 CDS 100% 4.950 3.465 N FAM20B n/a
10 TRCN0000278245 GCTGTCTTTGATTACCTGATT pLKO_005 1067 CDS 100% 4.950 3.465 N FAM20B n/a
11 TRCN0000195967 CCCTCTCTATCAGTGTTGCAT pLKO.1 1210 CDS 100% 3.000 2.100 N Fam20b n/a
12 TRCN0000137010 CCTTCCTAACTGTAGGAAACA pLKO.1 792 CDS 100% 0.495 0.347 N FAM20B n/a
13 TRCN0000138099 GCTTGTGCTGATGGAGACATA pLKO.1 860 CDS 100% 4.950 2.970 N FAM20B n/a
14 TRCN0000278244 GCTTGTGCTGATGGAGACATA pLKO_005 860 CDS 100% 4.950 2.970 N FAM20B n/a
15 TRCN0000162795 CTTCCAAAGTGCTGGGATTAT pLKO.1 2896 3UTR 100% 13.200 6.600 Y SLC48A1 n/a
16 TRCN0000128227 CAGGAGAATCACTTGAATCTA pLKO.1 4415 3UTR 100% 5.625 2.813 Y NLRP8 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017003002.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02270 pDONR223 100% 93.5% 93.5% None 377_445del;1282_1296del n/a
2 ccsbBroad304_02270 pLX_304 0% 93.5% 93.5% V5 377_445del;1282_1296del n/a
3 TRCN0000467320 TAACGCTCGCTATCCTTGATATCC pLX_317 31.1% 93.5% 93.5% V5 377_445del;1282_1296del n/a
Download CSV