Transcript: Mouse XM_006496736.2

PREDICTED: Mus musculus family with sequence similarity 20, member B (Fam20b), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Fam20b (215015)
Length:
4474
CDS:
379..1608

Additional Resources:

NCBI RefSeq record:
XM_006496736.2
NBCI Gene record:
Fam20b (215015)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006496736.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000425427 TAATCTGCGGACAGAAGTTAA pLKO_005 888 CDS 100% 13.200 18.480 N Fam20b n/a
2 TRCN0000431851 ACTTGTAATTCTCAACGCAAA pLKO_005 1601 CDS 100% 4.050 5.670 N Fam20b n/a
3 TRCN0000179166 GTTAGCAATACTCCTCGTCAT pLKO.1 405 CDS 100% 4.050 5.670 N Fam20b n/a
4 TRCN0000427214 TAAAGCTGACGTGGGCTATAA pLKO_005 669 CDS 100% 13.200 9.240 N Fam20b n/a
5 TRCN0000412386 TCCTAACTGTAGGGAACAATA pLKO_005 941 CDS 100% 13.200 9.240 N Fam20b n/a
6 TRCN0000431658 TCTAAAGAATGGAGTACTAAA pLKO_005 1410 CDS 100% 13.200 9.240 N Fam20b n/a
7 TRCN0000431604 ACAGCACAATCAATTCCAAAT pLKO_005 1654 3UTR 100% 10.800 7.560 N Fam20b n/a
8 TRCN0000195967 CCCTCTCTATCAGTGTTGCAT pLKO.1 1356 CDS 100% 3.000 2.100 N Fam20b n/a
9 TRCN0000183707 GCTTTACTGATTCTTGAAGGA pLKO.1 706 CDS 100% 2.640 1.848 N Fam20b n/a
10 TRCN0000183536 GCAGATATGTTAATCTGCGAA pLKO.1 878 CDS 100% 0.264 0.185 N Fam20b n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006496736.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02270 pDONR223 100% 89.7% 96% None (many diffs) n/a
2 ccsbBroad304_02270 pLX_304 0% 89.7% 96% V5 (many diffs) n/a
3 TRCN0000467320 TAACGCTCGCTATCCTTGATATCC pLX_317 31.1% 89.7% 96% V5 (many diffs) n/a
Download CSV