Construct: ORF ccsbBroadEn_02643

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017245.1_s300c1, BRDN0000394303
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RASSF1 (11186)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11186 RASSF1 Ras association domain fami... NM_001206957.1 100% 100%
2 human 11186 RASSF1 Ras association domain fami... NM_170712.3 100% 100%
3 human 11186 RASSF1 Ras association domain fami... XM_011533316.2 100% 100%
4 human 11186 RASSF1 Ras association domain fami... NM_170713.3 70% 70% 1_243del
5 human 11186 RASSF1 Ras association domain fami... XM_024453328.1 70% 70% 1_243del
6 human 11186 RASSF1 Ras association domain fami... NM_007182.5 55.5% 55.5% 1_453del
7 human 11186 RASSF1 Ras association domain fami... NM_170714.2 54.9% 54.9% 1_465del
8 mouse 56289 Rassf1 Ras association (RalGDS/AF-... XM_006511770.2 85% 94.1% (many diffs)
9 mouse 56289 Rassf1 Ras association (RalGDS/AF-... NM_019713.4 59.5% 65.9% (many diffs)
10 mouse 56289 Rassf1 Ras association (RalGDS/AF-... NM_001243748.1 47.3% 52.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
636
ORF length:
567
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GAGCTTGAAC AAGGACGGTT CTTACACAGG CTTCATCAAG GTTCAGCTGA
121AGCTGGTGCG CCCTGTCTCT GTGCCCTCCA GCAAGAAGCC ACCCTCCTTG CAGGATGCCC
181GGCGGGGCCC AGGACGGGGC ACAAGTGTCA GGCGCCGCAC TTCCTTTTAC CTGCCCAAGG
241ATGCTGTCAA GCACCTGCAT GTGCTGTCAC GCACAAGGGC ACGTGAAGTC ATTGAGGCCC
301TGCTGCGAAA GTTCTTGGTG GTGGATGACC CCCGCAAGTT TGCACTCTTT GAGCGCGCTG
361AGCGTCACGG CCAAGTGTAC TTGCGGAAGC TGTTGGATGA TGAGCAGCCC CTGCGGCTGC
421GGCTCCTGGC AGGGCCCAGT GACAAGGCCC TGAGCTTTGT CCTGAAGGAA AATGACTCTG
481GGGAGGTGAA CTGGGACGCC TTCAGCATGC CTGAACTACA TAACTTCCTA CGTATCCTGC
541AGCGGGAGGA GGAGGAGCAC CTCCGCCAGA TCCTGCAGAA GTACTCCTAT TGCCGCCAGA
601AGATCCAAGA GGCCCTGCAC GCCTGCCCCC TTGGGTTGCC AACTTTTTTG TACAAAGTtg
661gcattataag aaagcattgc ttatcaattt gttgcaacga ac

Download FASTA (ORF) (Full)