Construct: ORF ccsbBroadEn_02643
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017245.1_s300c1, BRDN0000394303
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RASSF1 (11186)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11186 | RASSF1 | Ras association domain fami... | NM_001206957.1 | 100% | 100% | |
| 2 | human | 11186 | RASSF1 | Ras association domain fami... | NM_170712.3 | 100% | 100% | |
| 3 | human | 11186 | RASSF1 | Ras association domain fami... | XM_011533316.2 | 100% | 100% | |
| 4 | human | 11186 | RASSF1 | Ras association domain fami... | NM_170713.3 | 70% | 70% | 1_243del |
| 5 | human | 11186 | RASSF1 | Ras association domain fami... | XM_024453328.1 | 70% | 70% | 1_243del |
| 6 | human | 11186 | RASSF1 | Ras association domain fami... | NM_007182.5 | 55.5% | 55.5% | 1_453del |
| 7 | human | 11186 | RASSF1 | Ras association domain fami... | NM_170714.2 | 54.9% | 54.9% | 1_465del |
| 8 | mouse | 56289 | Rassf1 | Ras association (RalGDS/AF-... | XM_006511770.2 | 85% | 94.1% | (many diffs) |
| 9 | mouse | 56289 | Rassf1 | Ras association (RalGDS/AF-... | NM_019713.4 | 59.5% | 65.9% | (many diffs) |
| 10 | mouse | 56289 | Rassf1 | Ras association (RalGDS/AF-... | NM_001243748.1 | 47.3% | 52.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 636
- ORF length:
- 567
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GAGCTTGAAC AAGGACGGTT CTTACACAGG CTTCATCAAG GTTCAGCTGA 121 AGCTGGTGCG CCCTGTCTCT GTGCCCTCCA GCAAGAAGCC ACCCTCCTTG CAGGATGCCC 181 GGCGGGGCCC AGGACGGGGC ACAAGTGTCA GGCGCCGCAC TTCCTTTTAC CTGCCCAAGG 241 ATGCTGTCAA GCACCTGCAT GTGCTGTCAC GCACAAGGGC ACGTGAAGTC ATTGAGGCCC 301 TGCTGCGAAA GTTCTTGGTG GTGGATGACC CCCGCAAGTT TGCACTCTTT GAGCGCGCTG 361 AGCGTCACGG CCAAGTGTAC TTGCGGAAGC TGTTGGATGA TGAGCAGCCC CTGCGGCTGC 421 GGCTCCTGGC AGGGCCCAGT GACAAGGCCC TGAGCTTTGT CCTGAAGGAA AATGACTCTG 481 GGGAGGTGAA CTGGGACGCC TTCAGCATGC CTGAACTACA TAACTTCCTA CGTATCCTGC 541 AGCGGGAGGA GGAGGAGCAC CTCCGCCAGA TCCTGCAGAA GTACTCCTAT TGCCGCCAGA 601 AGATCCAAGA GGCCCTGCAC GCCTGCCCCC TTGGGTTGCC AACTTTTTTG TACAAAGTtg 661 gcattataag aaagcattgc ttatcaattt gttgcaacga ac