Transcript: Human XM_024453328.1

PREDICTED: Homo sapiens Ras association domain family member 1 (RASSF1), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RASSF1 (11186)
Length:
2337
CDS:
730..1542

Additional Resources:

NCBI RefSeq record:
XM_024453328.1
NBCI Gene record:
RASSF1 (11186)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024453328.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000370824 AGTGCGCGCATTGCAAGTTCA pLKO_005 29 5UTR 100% 4.950 6.930 N RASSF1 n/a
2 TRCN0000077854 CGGTTCTTACACAGGCTTCAT pLKO.1 990 CDS 100% 4.950 6.930 N RASSF1 n/a
3 TRCN0000363640 CGGTTCTTACACAGGCTTCAT pLKO_005 990 CDS 100% 4.950 6.930 N RASSF1 n/a
4 TRCN0000077856 GCTTGAACAAGGACGGTTCTT pLKO.1 977 CDS 100% 4.950 6.930 N RASSF1 n/a
5 TRCN0000370825 AGACAGAAGTCTCCTCAATTT pLKO_005 1817 3UTR 100% 13.200 9.240 N RASSF1 n/a
6 TRCN0000336786 TCCTGCAGAAGTACTCCTATT pLKO_005 1475 CDS 100% 10.800 7.560 N RASSF1 n/a
7 TRCN0000077853 GCTCAGAATAAGGCAGGCATT pLKO.1 1978 3UTR 100% 4.050 2.835 N RASSF1 n/a
8 TRCN0000222721 CGAAAGTTCTTGGTGGTGGAT pLKO.1 1210 CDS 100% 2.640 1.848 N RASSF1 n/a
9 TRCN0000077857 GCCTGAACTACATAACTTCCT pLKO.1 1413 CDS 100% 2.640 1.848 N RASSF1 n/a
10 TRCN0000363586 GCCTGAACTACATAACTTCCT pLKO_005 1413 CDS 100% 2.640 1.848 N RASSF1 n/a
11 TRCN0000336709 AGAAGATCAAGGAGTACAATG pLKO_005 929 CDS 100% 10.800 6.480 N RASSF1 n/a
12 TRCN0000336788 GTGGTCTCAGGATATCCTTAT pLKO_005 1940 3UTR 100% 10.800 6.480 N RASSF1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024453328.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07766 pDONR223 99.7% 72.1% 64.9% None (many diffs) n/a
2 ccsbBroad304_07766 pLX_304 0% 72.1% 64.9% V5 (many diffs) n/a
3 TRCN0000481201 TAGCGAAGTCCGGTATTGCCACGC pLX_317 47.9% 72.1% 64.9% V5 (many diffs) n/a
4 ccsbBroadEn_02643 pDONR223 100% 70% 70% None 1_243del n/a
5 ccsbBroad304_02643 pLX_304 0% 70% 70% V5 1_243del n/a
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