Transcript: Mouse XM_006511770.2

PREDICTED: Mus musculus Ras association (RalGDS/AF-6) domain family member 1 (Rassf1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Rassf1 (56289)
Length:
1647
CDS:
359..928

Additional Resources:

NCBI RefSeq record:
XM_006511770.2
NBCI Gene record:
Rassf1 (56289)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006511770.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000305410 GCCGTCTACCTGTGGATATAG pLKO_005 1080 3UTR 100% 13.200 10.560 N Rassf1 n/a
2 TRCN0000054968 CCTCGCAAGTTTGCACTCTTT pLKO.1 620 CDS 100% 4.950 3.960 N Rassf1 n/a
3 TRCN0000305409 CCTCTTCATGAGCCTGAATAA pLKO_005 352 5UTR 100% 13.200 9.240 N Rassf1 n/a
4 TRCN0000054970 GCATGCCTGAACTGCACAATT pLKO.1 795 CDS 100% 13.200 9.240 N Rassf1 n/a
5 TRCN0000305348 ATCAAGGTTCAGCTGAAACTA pLKO_005 395 CDS 100% 5.625 3.938 N Rassf1 n/a
6 TRCN0000305408 GACTACAATGGCCAGATCAAC pLKO_005 326 5UTR 100% 4.950 3.465 N Rassf1 n/a
7 TRCN0000054969 GCACTCTTTGAGCGAACTGAA pLKO.1 632 CDS 100% 4.950 3.465 N Rassf1 n/a
8 TRCN0000309686 GCACTCTTTGAGCGAACTGAA pLKO_005 632 CDS 100% 4.950 3.465 N Rassf1 n/a
9 TRCN0000054971 GCTATTAAGCATCTGCATGTT pLKO.1 533 CDS 100% 4.950 3.465 N Rassf1 n/a
10 TRCN0000054972 GTATTCTCGTTGTCGCCAGAA pLKO.1 871 CDS 100% 4.050 2.835 N Rassf1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006511770.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02643 pDONR223 100% 85% 94.1% None (many diffs) n/a
2 ccsbBroad304_02643 pLX_304 0% 85% 94.1% V5 (many diffs) n/a
3 ccsbBroadEn_07766 pDONR223 99.7% 47.3% 52.3% None (many diffs) n/a
4 ccsbBroad304_07766 pLX_304 0% 47.3% 52.3% V5 (many diffs) n/a
5 TRCN0000481201 TAGCGAAGTCCGGTATTGCCACGC pLX_317 47.9% 47.3% 52.3% V5 (many diffs) n/a
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