Construct: ORF ccsbBroadEn_03035

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000958.1_s300c1, BRDN0000396580
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RABGEF1 (27342)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001287062.2 100% 100%
2 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367725.1 100% 100%
3 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367726.1 100% 100%
4 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367727.1 100% 100%
5 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367728.1 100% 100%
6 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367729.1 100% 100%
7 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367730.1 100% 100%
8 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367731.1 100% 100%
9 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367732.1 100% 100%
10 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367733.1 100% 100%
11 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367734.1 100% 100%
12 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367735.1 100% 100%
13 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367736.1 100% 100%
14 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_014504.3 100% 100%
15 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367737.1 99.7% 99.7% 346_348delGCA
16 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367738.1 99.7% 99.7% 346_348delGCA
17 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367739.1 99.7% 99.7% 346_348delGCA
18 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367740.1 99.7% 99.7% 346_348delGCA
19 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001287061.2 97.2% 97.2% 1_42del
20 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367741.1 92.6% 92.6% 595_711del
21 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367742.1 92.6% 92.6% 595_711del
22 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367743.1 87.4% 84% 0_1ins65;115_251del
23 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367744.1 87.4% 84% 0_1ins65;115_251del
24 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367756.1 84.3% 84.3% 136_137ins231
25 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367753.1 83% 83% 345_346ins249
26 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367754.1 74.2% 73% 1078_1081delGTGA;1101_1102ins376
27 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367755.1 74.2% 73.5% (many diffs)
28 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367752.1 74.1% 72.9% 346_348delGCA;1081_1084delGTGA;1104_1105ins376
29 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367745.1 66.1% 66.1% 0_1ins498
30 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367746.1 66.1% 66.1% 0_1ins498
31 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367747.1 66.1% 66.1% 0_1ins498
32 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367748.1 66.1% 66.1% 0_1ins498
33 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001287060.2 60.8% 60.8% 0_1ins576
34 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367722.1 60.8% 60.8% 0_1ins576
35 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367723.1 60.8% 60.8% 0_1ins576
36 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367724.1 60.8% 60.8% 0_1ins576
37 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367749.1 57.8% 57.8% 0_1ins621
38 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367750.1 57.8% 57.8% 0_1ins621
39 human 27342 RABGEF1 RAB guanine nucleotide exch... NM_001367751.1 57.8% 57.8% 0_1ins621
40 human 27342 RABGEF1 RAB guanine nucleotide exch... NR_160287.1 38.9% 1_77del;896_943del;1599_3781del
41 mouse 56715 Rabgef1 RAB guanine nucleotide exch... NM_001199059.1 88.6% 95.7% (many diffs)
42 mouse 56715 Rabgef1 RAB guanine nucleotide exch... NM_019983.2 88.6% 95.7% (many diffs)
43 mouse 56715 Rabgef1 RAB guanine nucleotide exch... XM_006504448.2 88.4% 95.5% (many diffs)
44 mouse 56715 Rabgef1 RAB guanine nucleotide exch... XM_006504447.3 85.4% 92.3% (many diffs)
45 mouse 56715 Rabgef1 RAB guanine nucleotide exch... XM_006504446.3 85.3% 92.1% (many diffs)
46 mouse 56715 Rabgef1 RAB guanine nucleotide exch... XM_017321033.1 53.7% 58.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1539
ORF length:
1473
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAG CCTTAAGTCT GAACGCCGAG GAATTCATGT GGATCAATCG GATCTCCTGT
121GCAAGAAAGG ATGTGGTTAC TACGGCAACC CTGCCTGGCA GGGTTTCTGC TCCAAGTGCT
181GGAGGGAAGA GTACCACAAA GCCAGGCAGA AGCAGATTCA GGAGGACTGG GAGCTGGCGG
241AGCGACTCCA GCGGGAGGAA GAAGAGGCCT TTGCCAGCAG TCAGAGCAGC CAAGGGGCCC
301AATCCCTCAC ATTCTCCAAG TTTGAAGAAA AGAAAACCAA CGAGAAGACC CGCAAGGTTA
361CCACAGTGAA GAAATTCTTC AGTGCATCTT CCAGGGTCGG ATCAAAGAAG GAAATTCAGG
421AAGCAAAAGC TCCCAGTCCT TCCATAAACC GGCAAACCAG CATTGAAACG GATAGAGTGT
481CTAAGGAGTT CATAGAATTT CTCAAGACCT TCCACAAGAC AGGCCAAGAA ATCTATAAAC
541AGACCAAGCT GTTTTTGGAA GGAATGCATT ACAAAAGGGA TCTAAGCATT GAAGAACAGT
601CAGAGTGTGC TCAGGATTTC TACCACAATG TGGCCGAAAG GATGCAAACT CGTGGGAAAG
661TGCCTCCAGA AAGAGTCGAG AAGATAATGG ATCAGATTGA AAAGTACATC ATGACTCGTC
721TCTATAAATA TGTATTCTGT CCAGAAACTA CTGATGATGA GAAGAAAGAT CTTGCCATTC
781AAAAGAGAAT CAGAGCCCTG CGCTGGGTTA CGCCTCAGAT GCTGTGTGTC CCTGTTAATG
841AAGACATCCC AGAAGTGTCT GATATGGTGG TGAAGGCGAT CACAGATATC ATTGAAATGG
901ATTCCAAGCG TGTGCCTCGA GACAAGCTGG CCTGCATCAC CAAGTGCAGC AAGCACATCT
961TCAATGCCAT CAAGATCACC AAGAATGAGC CGGCGTCAGC GGATGACTTC CTCCCCACCC
1021TCATCTACAT TGTTTTGAAG GGCAACCCCC CACGCCTTCA GTCTAATATC CAGTATATCA
1081CGCGCTTCTG CAATCCAAGC CGACTGATGA CTGGAGAGGA TGGCTACTAT TTCACCAATC
1141TGTGCTGTGC TGTGGCTTTC ATTGAGAAGC TAGACGCCCA GTCTTTGAAT CTAAGTCAGG
1201AGGATTTTGA TCGCTACATG TCTGGCCAGA CCTCTCCCAG GAAGCAAGAA GCTGAGAGTT
1261GGTCTCCTGA TGCTTGCTTA GGCGTCAAGC AAATGTATAA GAACTTGGAT CTCTTGTCTC
1321AGTTGAATGA ACGACAAGAA AGGATCATGA ATGAAGCCAA GAAACTGGAA AAAGACCTCA
1381TAGATTGGAC AGATGGAATT GCAAGAGAAG TTCAAGACAT CGTTGAGAAA TACCCACTGG
1441AAATTAAGCC TCCGAATCAA CCGTTAGCAG CTATTGACTC TGAAAACGTT GAAAATGATA
1501AACTTCCTCC ACCACTGCAA CCTCAAGTTT ATGCAGGATG CCCCACTTTC TTGTACAAAG
1561Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

Download FASTA (ORF) (Full)