Construct: ORF ccsbBroadEn_03145
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003946.1_s300c1, BRDN0000393784
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOX4 (50507)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50507 | NOX4 | NADPH oxidase 4 | NM_016931.5 | 99.9% | 99.8% | 945G>A |
| 2 | human | 50507 | NOX4 | NADPH oxidase 4 | NM_001143837.2 | 95.7% | 95.6% | 0_1ins72;873G>A |
| 3 | human | 50507 | NOX4 | NADPH oxidase 4 | XM_011542857.2 | 95.7% | 95.6% | 0_1ins72;873G>A |
| 4 | human | 50507 | NOX4 | NADPH oxidase 4 | NM_001291927.1 | 94.7% | 94% | (many diffs) |
| 5 | human | 50507 | NOX4 | NADPH oxidase 4 | NM_001291929.2 | 94.2% | 87.8% | (many diffs) |
| 6 | human | 50507 | NOX4 | NADPH oxidase 4 | NM_001143836.3 | 93% | 92.9% | 945G>A;1217_1218ins120 |
| 7 | human | 50507 | NOX4 | NADPH oxidase 4 | XM_017017841.2 | 90.2% | 89.3% | (many diffs) |
| 8 | human | 50507 | NOX4 | NADPH oxidase 4 | NM_001300995.1 | 88.8% | 88.7% | 0_1ins72;873G>A;1145_1146ins120 |
| 9 | human | 50507 | NOX4 | NADPH oxidase 4 | XM_006718849.4 | 88% | 87.2% | (many diffs) |
| 10 | human | 50507 | NOX4 | NADPH oxidase 4 | NM_001291926.2 | 87.1% | 87% | 0_1ins222;723G>A |
| 11 | human | 50507 | NOX4 | NADPH oxidase 4 | XM_017017844.1 | 71.2% | 71.1% | 0_1ins498;447G>A |
| 12 | human | 50507 | NOX4 | NADPH oxidase 4 | XM_017017845.1 | 71.2% | 71.1% | 0_1ins498;447G>A |
| 13 | human | 50507 | NOX4 | NADPH oxidase 4 | XM_017017842.1 | 68.1% | 61.2% | (many diffs) |
| 14 | human | 50507 | NOX4 | NADPH oxidase 4 | XM_017017843.2 | 64.3% | 61.1% | (many diffs) |
| 15 | mouse | 50490 | Nox4 | NADPH oxidase 4 | NM_015760.5 | 87% | 90.8% | (many diffs) |
| 16 | mouse | 50490 | Nox4 | NADPH oxidase 4 | XM_017322347.1 | 86.8% | 90.6% | (many diffs) |
| 17 | mouse | 50490 | Nox4 | NADPH oxidase 4 | XM_006508010.2 | 83.8% | 83.1% | (many diffs) |
| 18 | mouse | 50490 | Nox4 | NADPH oxidase 4 | XM_011241854.2 | 81.6% | 85.1% | (many diffs) |
| 19 | mouse | 50490 | Nox4 | NADPH oxidase 4 | XM_006508012.1 | 81.5% | 85% | (many diffs) |
| 20 | mouse | 50490 | Nox4 | NADPH oxidase 4 | NM_001285835.1 | 75.7% | 78.7% | (many diffs) |
| 21 | mouse | 50490 | Nox4 | NADPH oxidase 4 | NM_001285833.1 | 71% | 73.7% | (many diffs) |
| 22 | mouse | 50490 | Nox4 | NADPH oxidase 4 | XM_006508011.3 | 53.6% | 54.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1800
- ORF length:
- 1734
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC TGTGTCCTGG AGGAGCTGGC TCGCCAACGA AGGGGTTAAA CACCTCTGCC 121 TGTTCATCTG GCTCTCCATG AATGTCCTGC TTTTCTGGAA AACCTTCTTG CTGTATAACC 181 AAGGGCCAGA GTATCACTAC CTCCACCAGA TGTTGGGGCT AGGATTGTGT CTAAGCAGAG 241 CCTCAGCATC TGTTCTTAAC CTCAACTGCA GCCTTATCCT TTTACCCATG TGCCGAACAC 301 TCTTGGCTTA CCTCCGAGGA TCACAGAAGG TTCCAAGCAG GAGAACCAGG AGATTGTTGG 361 ATAAAAGCAG AACATTCCAT ATTACCTGTG GTGTTACTAT CTGTATTTTC TCAGGCGTGC 421 ATGTGGCTGC CCATCTGGTG AATGCCCTCA ACTTCTCAGT GAATTACAGT GAAGACTTTG 481 TTGAACTGAA TGCAGCAAGA TACCGAGATG AGGATCCTAG AAAACTTCTC TTCACAACTG 541 TTCCTGGCCT GACAGGGGTC TGCATGGTGG TGGTGCTATT CCTCATGATC ACAGCCTCTA 601 CATATGCAAT AAGAGTTTCT AACTATGATA TCTTCTGGTA TACTCATAAC CTCTTCTTTG 661 TCTTCTACAT GCTGCTGACG TTGCATGTTT CAGGAGGGCT GCTGAAGTAT CAAACTAATT 721 TAGATACCCA CCCTCCCGGC TGCATCAGTC TTAACCGAAC CAGCTCTCAG AATATTTCCT 781 TACCAGAGTA TTTCTCAGAA CATTTTCATG AACCTTTCCC TGAAGGATTT TCAAAACCGG 841 CAGAGTTTAC CCAGCACAAA TTTGTGAAGA TTTGTATGGA AGAGCCCAGA TTCCAAGCTA 901 ATTTTCCACA GACTTGGCTT TGGATTTCTG GACCTTTGTG CCTGTACTGT GCCGAAAGAC 961 TTTACAGGTA TATCCGGAGC AATAAGCCAG TCACCATCAT TTCGGTCATA AGTCATCCCT 1021 CAGATGTCAT GGAAATCCGA ATGGTCAAAG AAAATTTTAA AGCAAGACCT GGTCAGTATA 1081 TTACTCTACA TTGTCCCAGT GTATCTGCAT TAGAAAATCA TCCATTTACC CTCACAATGT 1141 GTCCAACTGA AACCAAAGCA ACATTTGGGG TTCATCTTAA AATAGTAGGA GACTGGACAG 1201 AACGATTTCG AGATTTACTA CTGCCTCCAT CTAGTCAAGA CTCCGAAATT CTGCCCTTCA 1261 TTCAATCTAG AAATTATCCC AAGCTGTATA TTGATGGTCC TTTTGGAAGT CCATTTGAGG 1321 AATCACTGAA CTATGAGGTC AGCCTCTGCG TGGCTGGAGG CATTGGAGTA ACTCCATTTG 1381 CATCAATACT CAACACCCTG TTGGATGACT GGAAACCATA CAAGCTTAGA AGACTATACT 1441 TTATTTGGGT ATGCAGAGAT ATCCAGTCCT TCCGTTGGTT TGCAGATTTA CTCTGTATGT 1501 TGCATAACAA GTTTTGGCAA GAGAACAGAC CTGACTATGT CAACATCCAG CTGTACCTCA 1561 GTCAAACAGA TGGGATACAG AAGATAATTG GAGAAAAATA TCATGCACTG AATTCAAGAC 1621 TGTTTATAGG ACGTCCTCGG TGGAAACTTT TGTTTGATGA AATAGCAAAA TATAACAGAG 1681 GAAAAACAGT TGGTGTTTTC TGTTGTGGAC CCAATTCACT ATCCAAGACT CTTCATAAAC 1741 TGAGTAACCA GAACAACTCA TATGGGACAA GATTTGAATA CAATAAAGAG TCTTTCAGCT 1801 GCCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca 1861 acgaac