Construct: ORF ccsbBroadEn_03358
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001440.1_s300c1, BRDN0000384991
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MPC1 (51660)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51660 | MPC1 | mitochondrial pyruvate carr... | NM_016098.4 | 100% | 100% | |
| 2 | human | 51660 | MPC1 | mitochondrial pyruvate carr... | NM_001270879.2 | 60.5% | 60.5% | 0_1ins129 |
| 3 | human | 51660 | MPC1 | mitochondrial pyruvate carr... | XM_011535895.2 | 60.5% | 60.5% | 0_1ins129 |
| 4 | human | 51660 | MPC1 | mitochondrial pyruvate carr... | XM_024446456.1 | 60.5% | 60.5% | 0_1ins129 |
| 5 | human | 51660 | MPC1 | mitochondrial pyruvate carr... | NR_073093.2 | 28.8% | 1_64del;140_368del;621_1133del | |
| 6 | mouse | 55951 | Mpc1 | mitochondrial pyruvate carr... | NM_018819.4 | 90.2% | 94.4% | (many diffs) |
| 7 | mouse | 55951 | Mpc1 | mitochondrial pyruvate carr... | XM_011246219.2 | 71.3% | 71.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 393
- ORF length:
- 327
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC GGGCGCGTTG GTGCGGAAAG CGGCGGACTA TGTCCGAAGC AAGGATTTCC 121 GGGACTACCT CATGAGTACG CACTTCTGGG GCCCAGTAGC CAACTGGGGT CTTCCCATTG 181 CTGCCATCAA TGATATGAAA AAGTCTCCAG AGATTATCAG TGGGCGGATG ACATTTGCCC 241 TCTGTTGCTA TTCTTTGACA TTCATGAGAT TTGCCTACAA GGTACAGCCT CGGAACTGGC 301 TTCTGTTTGC ATGCCACGCA ACAAATGAAG TAGCCCAGCT CATCCAGGGA GGGCGGCTTA 361 TCAAACACGA GATGACTAAA ACGGCATCTG CATACCCAAC TTTCTTGTAC AAAGTtggca 421 ttataagaaa gcattgctta tcaatttgtt gcaacgaac