Construct: ORF ccsbBroadEn_03643

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010855.1_s300c1, BRDN0000386639
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
VPS35 (55737)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55737 VPS35 VPS35 retromer complex comp... NM_018206.6 99.9% 100% 1938C>T
2 human 55737 VPS35 VPS35 retromer complex comp... XM_011523227.3 96.3% 96.3% 0_1ins87;1851C>T
3 human 55737 VPS35 VPS35 retromer complex comp... XM_005256045.3 91.5% 91.5% 0_1ins201;1737C>T
4 mouse 65114 Vps35 VPS35 retromer complex comp... NM_022997.4 91.4% 99.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2454
ORF length:
2388
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCC TACAACACAG CAGTCCCCTC AGGATGAGCA GGAAAAGCTC TTGGATGAAG
121CCATACAGGC TGTGAAGGTC CAGTCATTCC AAATGAAGAG ATGCCTGGAC AAAAACAAGC
181TTATGGATGC TCTAAAACAT GCTTCTAATA TGCTTGGTGA ACTCCGGACT TCTATGTTAT
241CACCAAAGAG TTACTATGAA CTTTATATGG CCATTTCTGA TGAACTGCAC TACTTGGAGG
301TCTACCTGAC AGATGAGTTT GCTAAAGGAA GGAAAGTGGC AGATCTCTAC GAACTTGTAC
361AGTATGCTGG AAACATTATC CCAAGGCTTT ACCTTTTGAT CACAGTTGGA GTTGTATATG
421TCAAGTCATT TCCTCAGTCC AGGAAGGATA TTTTGAAAGA TTTGGTAGAA ATGTGCCGTG
481GTGTGCAACA TCCCTTGAGG GGTCTGTTTC TTCGAAATTA CCTTCTTCAG TGTACCAGAA
541ATATCTTACC TGATGAAGGA GAGCCAACAG ATGAAGAAAC AACTGGTGAC ATCAGTGATT
601CCATGGATTT TGTACTGCTC AACTTTGCAG AAATGAACAA GCTCTGGGTG CGAATGCAGC
661ATCAGGGACA TAGCCGAGAT AGAGAAAAAA GAGAACGAGA AAGACAAGAA CTGAGAATTT
721TAGTGGGAAC AAATTTGGTG CGCCTCAGTC AGTTGGAAGG TGTAAATGTG GAACGTTACA
781AACAGATTGT TTTGACTGGC ATATTGGAGC AAGTTGTAAA CTGTAGGGAT GCTTTGGCTC
841AAGAATATCT CATGGAGTGT ATTATTCAGG TTTTCCCTGA TGAATTTCAC CTCCAGACTT
901TGAATCCTTT TCTTCGGGCC TGTGCTGAGT TACACCAGAA TGTAAATGTG AAGAACATAA
961TCATTGCTTT AATTGATAGA TTAGCTTTAT TTGCTCACCG TGAAGATGGA CCTGGAATCC
1021CAGCGGATAT TAAACTTTTT GATATATTTT CACAGCAGGT GGCTACAGTG ATACAGTCTA
1081GACAAGACAT GCCTTCAGAG GATGTTGTAT CTTTACAAGT CTCTCTGATT AATCTTGCCA
1141TGAAATGTTA CCCTGATCGT GTGGACTATG TTGATAAAGT TCTAGAAACA ACAGTGGAGA
1201TATTCAATAA GCTCAACCTT GAACATATTG CTACCAGTAG TGCAGTTTCA AAGGAACTCA
1261CCAGACTTTT GAAAATACCA GTTGACACTT ACAACAATAT TTTAACAGTC TTGAAATTAA
1321AACATTTTCA CCCACTCTTT GAGTACTTTG ACTACGAGTC CAGAAAGAGC ATGAGTTGTT
1381ATGTGCTTAG TAATGTTCTG GATTATAACA CAGAAATTGT CTCTCAAGAC CAGGTGGATT
1441CCATAATGAA TTTGGTATCC ACGTTGATTC AAGATCAGCC AGATCAACCT GTAGAAGACC
1501CTGATCCAGA AGATTTTGCT GATGAGCAGA GCCTTGTGGG CCGCTTCATT CATCTGCTGC
1561GCTCTGAGGA CCCTGACCAG CAGTACTTGA TTTTGAACAC AGCACGAAAA CATTTTGGAG
1621CTGGTGGAAA TCAGCGGATT CGCTTCACAC TGCCACCTTT GGTATTTGCA GCTTACCAGC
1681TGGCTTTTCG ATATAAAGAG AATTCTAAAG TGGATGACAA ATGGGAAAAG AAATGCCAGA
1741AGATTTTTTC ATTTGCCCAC CAGACTATCA GTGCTTTGAT CAAAGCAGAG CTGGCAGAAT
1801TGCCCTTAAG ACTTTTTCTT CAAGGAGCAC TAGCTGCTGG GGAAATTGGT TTTGAAAATC
1861ATGAGACAGT CGCATATGAA TTCATGTCCC AGGCATTTTC TCTGTATGAA GATGAAATCA
1921GCGATTCCAA AGCACAGCTA GCTGCCATCA CCTTGATCAT TGGCACTTTT GAAAGGATGA
1981AGTGCTTCAG TGAAGAGAAT CATGAACCTC TGAGGACTCA GTGTGCCCTT GCTGCATCCA
2041AACTTCTAAA GAAACCTGAT CAGGGCCGAG CTGTGAGCAC CTGTGCACAT CTCTTCTGGT
2101CTGGCAGAAA CACGGACAAA AATGGGGAGG AGCTTCACGG AGGCAAGAGG GTAATGGAGT
2161GCCTAAAAAA AGCTCTAAAA ATAGCAAATC AGTGCATGGA CCCCTCTCTA CAAGTGCAGC
2221TTTTTATAGA AATTCTGAAC AGATATATCT ATTTTTATGA AAAGGAAAAT GATGCGGTAA
2281CAATTCAGGT TTTAAACCAG CTTATCCAAA AGATTCGAGA AGACCTCCCG AATCTTGAAT
2341CCAGTGAAGA AACAGAGCAG ATTAACAAAC ATTTTCATAA CACACTGGAG CATTTGCGCT
2401TGCGGCGGGA ATCACCAGAA TCCGAGGGGC CAATTTATGA AGGTCTCATC CTTTACCCAA
2461TTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)

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