Construct: ORF ccsbBroadEn_03755

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018466.1_s300c1, BRDN0000387483
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IFT46 (56912)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56912 IFT46 intraflagellar transport 46 NM_001168618.1 100% 100%
2 human 56912 IFT46 intraflagellar transport 46 XM_011542906.3 100% 100%
3 human 56912 IFT46 intraflagellar transport 46 XM_017018018.2 100% 100%
4 human 56912 IFT46 intraflagellar transport 46 NM_020153.3 85.6% 85.6% 43_195del
5 human 56912 IFT46 intraflagellar transport 46 XM_011542905.3 85.6% 85.6% 43_195del
6 human 56912 IFT46 intraflagellar transport 46 XM_017018017.1 85.6% 85.6% 43_195del
7 mouse 76568 Ift46 intraflagellar transport 46 NM_023831.3 85.9% 94% (many diffs)
8 mouse 76568 Ift46 intraflagellar transport 46 XM_006510671.3 85.9% 94% (many diffs)
9 mouse 76568 Ift46 intraflagellar transport 46 XM_006510672.3 63.8% 70.3% (many diffs)
10 mouse 76568 Ift46 intraflagellar transport 46 XR_001779069.1 45.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
978
ORF length:
912
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC TGATAACAGC AGTGATGAGT GTGAAGAGGA AAATAACAAG GAGAAGAAGA
121AGACCTCACA GTTGACACCT CAACGGGGCT TTAGTGAAAA TGAGGATGAC GATGATGATG
181ATGATGATTC ATCTGAAACT GATTCTGATT CTGATGATGA TGATGAAGAG CATGGAGCCC
241CTCTGGAAGG GGCCTATGAC CCTGCAGACT ATGAGCATTT GCCAGTTTCT GCTGAAATTA
301AGGAACTCTT CCAGTACATC AGTAGGTACA CACCTCAGTT GATTGACCTG GACCACAAAC
361TGAAGCCTTT CATTCCTGAT TTTATCCCAG CTGTCGGGGA TATTGATGCA TTCTTAAAGG
421TCCCACGTCC TGATGGAAAG CCTGACAACC TTGGCCTATT GGTATTGGAT GAACCTTCTA
481CAAAGCAGTC AGACCCTACG GTGCTCTCAC TCTGGTTAAC AGAGAATTCT AAGCAGCACA
541ACATCACACA ACATATGAAA GTAAAAAGCC TAGAAGATGC AGAAAAGAAT CCCAAAGCCA
601TTGACACGTG GATTGAGAGC ATCTCTGAAT TACACCGTTC TAAGCCCCCT GCGACTGTGC
661ACTACACCAG GCCCATGCCC GACATTGACA CGCTGATGCA GGAATGGTCC CCGGAGTTTG
721AAGAGCTTTT GGGCAAGGTA AGCCTGCCCA CGGCAGAGAT TGATTGCAGC CTGGCAGAGT
781ACATTGACAT GATCTGTGCC ATTCTAGACA TCCCTGTCTA CAAGAGTCGG ATCCAGTCCC
841TCCATCTGCT CTTTTCCCTC TACTCAGAAT TCAAGAACTC ACAGCATTTT AAAGCTCTCG
901CTGAAGGCAA GAAAGCATTC ACTCCTTCAT CCAATTCCAC CTCCCAAGCT GGAGACATGG
961AGACATTAAC CTTCAGCTGC CCAACTTTCT TGTACAAAGT tggcattata agaaagcatt
1021gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)