Construct: ORF ccsbBroadEn_03814

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002296.1_s300c1, BRDN0000397316
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RHOJ (57381)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57381 RHOJ ras homolog family member J NM_020663.5 100% 100%
2 human 57381 RHOJ ras homolog family member J XM_006720214.4 80.9% 78.5% (many diffs)
3 human 57381 RHOJ ras homolog family member J XM_011536993.3 74.2% 74.2% 234_235ins165
4 human 57381 RHOJ ras homolog family member J XR_245709.4 18.7% 1_458del;957_1076del;1221_3418del
5 human 57381 RHOJ ras homolog family member J XR_245708.4 18.7% 1_458del;956_1085del;1231_3428del
6 mouse 80837 Rhoj ras homolog family member J NM_023275.2 87.8% 92.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
708
ORF length:
642
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA CTGCAAAGAG GGAACTGACA GCAGCTGCGG CTGCAGGGGC AACGACGAGA
121AGAAGATGTT GAAGTGTGTG GTGGTGGGGG ACGGTGCCGT GGGGAAAACC TGCCTGCTGA
181TGAGCTACGC CAACGACGCC TTCCCAGAGG AATACGTGCC CACTGTGTTT GACCACTATG
241CAGTTACTGT GACTGTGGGA GGCAAGCAAC ACTTGCTCGG ACTGTATGAC ACCGCGGGAC
301AGGAGGACTA CAACCAGCTG AGGCCACTCT CCTACCCCAA CACGGATGTG TTTTTGATCT
361GCTTCTCTGT CGTAAACCCT GCCTCTTACC ACAATGTCCA GGAGGAATGG GTCCCCGAGC
421TCAAGGACTG CATGCCTCAC GTGCCTTATG TCCTCATAGG GACCCAGATT GATCTCCGTG
481ATGACCCAAA AACCTTGGCC CGTTTGCTGT ATATGAAAGA GAAACCTCTC ACTTACGAGC
541ATGGTGTGAA GCTCGCAAAA GCGATCGGAG CACAGTGCTA CTTGGAATGT TCAGCTCTGA
601CTCAGAAAGG TCTCAAAGCG GTTTTTGATG AAGCAATCCT CACCATTTTC CACCCCAAGA
661AAAAGAAGAA ACGCTGTTCT GAGGGTCACA GCTGCTGTTC AATTATCTGC CCAACTTTCT
721TGTACAAAGT tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)