Construct: ORF ccsbBroadEn_04116

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002403.1_s300c1, BRDN0000397455
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LIN28A (79727)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79727 LIN28A lin-28 homolog A NM_024674.6 100% 100%
2 human 79727 LIN28A lin-28 homolog A XM_011542148.2 100% 100%
3 mouse 83557 Lin28a lin-28 homolog A (C. elegans) NM_145833.1 91% 96.6% (many diffs)
4 mouse 83557 Lin28a lin-28 homolog A (C. elegans) XM_011250361.2 69.3% 73.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
693
ORF length:
627
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGG CTCCGTGTCC AACCAGCAGT TTGCAGGTGG CTGCGCCAAG GCGGCAGAAG
121AGGCGCCCGA GGAGGCGCCG GAGGACGCGG CCCGGGCGGC GGACGAGCCT CAGCTGCTGC
181ACGGTGCGGG CATCTGTAAG TGGTTCAACG TGCGCATGGG GTTCGGCTTC CTGTCCATGA
241CCGCCCGCGC CGGGGTCGCG CTCGACCCCC CAGTGGATGT CTTTGTGCAC CAGAGTAAGC
301TGCACATGGA AGGGTTCCGG AGCTTGAAGG AGGGTGAGGC AGTGGAGTTC ACCTTTAAGA
361AGTCAGCCAA GGGTCTGGAA TCCATCCGTG TCACCGGACC TGGTGGAGTA TTCTGTATTG
421GGAGTGAGAG GCGGCCAAAA GGAAAGAGCA TGCAGAAGCG CAGATCAAAA GGAGACAGGT
481GCTACAACTG TGGAGGTCTA GATCATCATG CCAAGGAATG CAAGCTGCCA CCCCAGCCCA
541AGAAGTGCCA CTTCTGCCAG AGCATCAGCC ATATGGTAGC CTCATGTCCG CTGAAGGCCC
601AGCAGGGCCC TAGTGCACAG GGAAAGCCAA CCTACTTTCG AGAGGAAGAA GAAGAAATCC
661ACAGCCCTAC CCTGCTCCCG GAGGCACAGA ATTGCCCAAC TTTCTTGTAC AAAGTtggca
721ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)