Construct: ORF ccsbBroadEn_04128
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013752.1_s300c1, BRDN0000396688
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALG9 (79796)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_024740.2 | 100% | 100% | |
| 2 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001077690.1 | 98.8% | 98.8% | 1170_1171ins21 |
| 3 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XM_005277723.5 | 98.1% | 98% | 1853_1887delinsGT |
| 4 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001352417.1 | 97% | 96.9% | 1170_1171ins21;1832_1866delinsGT |
| 5 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XM_006718913.3 | 94.6% | 93.5% | (many diffs) |
| 6 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XM_024448695.1 | 93.5% | 92.4% | (many diffs) |
| 7 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XM_011542992.2 | 93.4% | 92.8% | (many diffs) |
| 8 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XM_017018314.2 | 93.3% | 93.3% | 892_893ins123 |
| 9 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XM_017018313.2 | 92.3% | 91.7% | (many diffs) |
| 10 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001352418.1 | 92.2% | 92.2% | 892_893ins123;1047_1048ins21 |
| 11 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XR_001747980.1 | 83.7% | (many diffs) | |
| 12 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XR_001747970.2 | 83.1% | (many diffs) | |
| 13 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XR_947863.3 | 81.8% | 1_88del;1819_2040del;2165_2266del | |
| 14 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XR_947864.2 | 81.5% | 1_88del;1105_1106ins176;1767_1882del | |
| 15 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XR_947865.2 | 76.7% | (many diffs) | |
| 16 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XR_001747967.2 | 74% | 1_88del;1941_2502delinsGT | |
| 17 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XR_001747973.1 | 73.6% | 1_99del;370_373delGTAG;1956_2513delinsGT | |
| 18 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XR_001747968.2 | 73.2% | 1_88del;1258_1259ins21;1920_2480delinsGT | |
| 19 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001077691.2 | 72.3% | 72.3% | 0_1ins513 |
| 20 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001352413.1 | 72.3% | 72.3% | 0_1ins513 |
| 21 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001352414.2 | 72.3% | 72.3% | 0_1ins513 |
| 22 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XR_001747979.1 | 71.9% | (many diffs) | |
| 23 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001077692.2 | 71.1% | 71.1% | 0_1ins513;657_658ins21 |
| 24 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001352409.1 | 71.1% | 71.1% | 0_1ins513;657_658ins21 |
| 25 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001352410.1 | 71.1% | 71.1% | 0_1ins513;657_658ins21 |
| 26 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001352411.2 | 71.1% | 71.1% | 0_1ins513;657_658ins21 |
| 27 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001352412.2 | 71.1% | 71.1% | 0_1ins513;657_658ins21 |
| 28 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001352419.1 | 70.9% | 70.9% | 0_1ins513;1340_1374delinsGT |
| 29 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001352415.1 | 69.8% | 69.7% | 0_1ins513;657_658ins21;1319_1353delinsGT |
| 30 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001352416.1 | 69.8% | 69.7% | 0_1ins513;657_658ins21;1319_1353delinsGT |
| 31 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XR_001747974.1 | 68.8% | (many diffs) | |
| 32 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001352422.2 | 67.1% | 67.1% | 0_1ins588;582_583ins21 |
| 33 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001352421.2 | 66% | 65% | (many diffs) |
| 34 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001352420.2 | 65.4% | 64.6% | (many diffs) |
| 35 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XR_001747971.1 | 64.7% | (many diffs) | |
| 36 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XR_001747972.1 | 64.6% | (many diffs) | |
| 37 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NM_001352423.2 | 64.5% | 64.5% | 0_1ins513;379_380ins123;534_535ins21 |
| 38 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XR_001747975.1 | 64% | (many diffs) | |
| 39 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XR_001747976.1 | 63.9% | (many diffs) | |
| 40 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XR_001747969.2 | 60.7% | 1_88del;980_981ins123;1818_2723delinsGT | |
| 41 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | XR_001747977.1 | 56.1% | (many diffs) | |
| 42 | human | 79796 | ALG9 | ALG9 alpha-1,2-mannosyltran... | NR_147984.2 | 27.2% | (many diffs) | |
| 43 | mouse | 102580 | Alg9 | asparagine-linked glycosyla... | NM_133981.2 | 86.3% | 92.4% | (many diffs) |
| 44 | mouse | 102580 | Alg9 | asparagine-linked glycosyla... | XM_006509902.1 | 80.4% | 85.9% | (many diffs) |
| 45 | mouse | 102580 | Alg9 | asparagine-linked glycosyla... | XM_006509901.1 | 79.9% | 79.7% | (many diffs) |
| 46 | mouse | 102580 | Alg9 | asparagine-linked glycosyla... | XM_006509903.3 | 72.5% | 69.6% | (many diffs) |
| 47 | mouse | 102580 | Alg9 | asparagine-linked glycosyla... | XM_017313065.1 | 66.6% | 63.3% | (many diffs) |
| 48 | mouse | 102580 | Alg9 | asparagine-linked glycosyla... | NR_045601.1 | 54.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1920
- ORF length:
- 1854
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC TAGTCGAGGG GCTCGGCAGC GCCTGAAGGG CAGCGGGGCC AGCAGTGGGG 121 ATACGGCCCC GGCTGCGGAC AAGCTGCGGG AGCTGCTGGG CAGCCGAGAG GCGGGCGGCG 181 CGGAGCACCG GACCGAGTTA TCTGGGAACA AAGCAGGACA AGTCTGGGCA CCTGAAGGAT 241 CTACTGCTTT CAAGTGTCTG CTTTCAGCAA GGTTATGTGC TGCTCTCCTG AGCAACATCT 301 CTGACTGTGA TGAAACATTC AACTACTGGG AGCCAACACA CTACCTCATC TATGGGGAAG 361 GGTTTCAGAC TTGGGAATAT TCCCCAGCAT ATGCCATTCG CTCCTATGCT TACCTGTTGC 421 TTCATGCCTG GCCAGCTGCA TTTCATGCAA GAATTCTACA AACTAATAAG ATTCTTGTGT 481 TTTACTTTTT GCGATGTCTT CTGGCTTTTG TGAGCTGTAT TTGTGAACTT TACTTTTACA 541 AGGCTGTGTG CAAGAAGTTT GGGTTGCACG TGAGTCGAAT GATGCTAGCC TTCTTGGTTC 601 TCAGCACTGG CATGTTTTGC TCATCATCAG CATTCCTTCC TAGTAGCTTC TGTATGTACA 661 CTACGTTGAT AGCCATGACT GGATGGTATA TGGACAAGAC TTCCATTGCT GTGCTGGGAG 721 TAGCAGCTGG GGCTATCTTA GGCTGGCCAT TCAGTGCAGC TCTTGGTTTA CCCATTGCCT 781 TTGATTTGCT GGTCATGAAA CACAGGTGGA AGAGTTTCTT TCATTGGTCG CTGATGGCCC 841 TCATACTATT TCTGGTGCCT GTGGTGGTCA TTGACAGCTA CTATTATGGG AAGTTGGTGA 901 TTGCACCACT CAACATTGTT TTGTATAATG TCTTTACTCC TCATGGACCT GATCTTTATG 961 GTACAGAACC CTGGTATTTC TATTTAATTA ATGGATTTCT GAATTTCAAT GTAGCCTTTG 1021 CTTTGGCTCT CCTAGTCCTA CCACTGACTT CTCTTATGGA ATACCTGCTG CAGAGATTTC 1081 ATGTTCAGAA TTTAGGCCAC CCGTATTGGC TTACCTTGGC TCCAATGTAT ATTTGGTTTA 1141 TAATTTTCTT CATCCAGCCT CACAAAGAGG AGAGATTTCT TTTCCCTGTG TATCCACTTA 1201 TATGTCTCTG TGGCGCTGTG GCTCTCTCTG CACTTCAGCA CAGTTTTCTG TACTTCCAGA 1261 AATGTTACCA CTTTGTGTTT CAACGATATC GCCTGGAGCA CTATACTGTG ACATCGAATT 1321 GGCTGGCATT AGGAACTGTC TTCCTGTTTG GGCTCTTGTC ATTTTCTCGC TCTGTGGCAC 1381 TGTTCAGAGG ATATCACGGG CCCCTTGATT TGTATCCAGA ATTTTACCGA ATTGCTACAG 1441 ACCCAACCAT CCACACTGTC CCAGAAGGCA GACCTGTGAA TGTCTGTGTG GGAAAAGAGT 1501 GGTATCGATT TCCCAGCAGC TTCCTTCTTC CTGACAATTG GCAGCTTCAG TTCATTCCAT 1561 CAGAGTTCAG AGGTCAGTTA CCAAAACCTT TTGCAGAAGG ACCTCTGGCC ACCCGGATTG 1621 TTCCTACTGA CATGAATGAC CAGAATCTAG AAGAGCCATC CAGATATATT GATATCAGTA 1681 AATGCCATTA TTTAGTGGAT TTGGACACCA TGAGAGAAAC ACCCCGGGAG CCAAAATATT 1741 CATCCAATAA AGAAGAATGG ATCAGCTTGG CCTATAGACC ATTCCTTGAT GCTTCTAGAT 1801 CTTCAAAGCT GCTGCGGGCA TTCTATGTCC CCTTCCTGTC AGATCAGTAT ACAGTGTACG 1861 TAAACTACAC CATCCTCAAA CCCCGGAAAG CAAAGCAAAT CAGGAAGAAA AGTGGAGGTT 1921 ACCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca 1981 acgaac