Construct: ORF ccsbBroadEn_04241
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012342.1_s300c1, BRDN0000388748
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDRG1 (81572)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81572 | PDRG1 | p53 and DNA damage regulated 1 | NM_030815.3 | 100% | 100% | |
| 2 | mouse | 68559 | Pdrg1 | p53 and DNA damage regulated 1 | NM_178939.2 | 88.2% | 91.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 465
- ORF length:
- 399
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCT ATCACCCGAG GCAGAGCGAG TGCTGCGGTA CCTTGTAGAA GTGGAGGAGC 121 TCGCCGAGGA GGTGCTGGCG GACAAGCGGC AGATTGTGGA CCTGGACACT AAAAGGAATC 181 AGAATCGAGA GGGCCTGAGG GCCCTGCAGA AGGATCTCAG CCTCTCTGAA GATGTGATGG 241 TTTGCTTCGG GAACATGTTT ATCAAGATGC CTCACCCTGA GACAAAGGAA ATGATTGAAA 301 AAGATCAAGA TCATCTGGAT AAAGAAATAG AAAAACTGCG GAAGCAACTT AAAGTGAAGG 361 TCAACCGCCT TTTTGAGGCC CAAGGCAAAC CGGAGCTGAA GGGTTTTAAC TTGAACCCCC 421 TCAACCAGGA TGAGCTTAAA GCTCTCAAGG TCATCTTGAA AGGATGCCCA ACTTTCTTGT 481 ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c