Construct: ORF ccsbBroadEn_04474

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013748.1_s300c1, BRDN0000394996
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KRTAP4-2 (85291)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 85291 KRTAP4-2 keratin associated protein 4-2 NM_033062.4 99.7% 99.2% 284A>G
2 human 100132476 KRTAP4-7 keratin associated protein 4-7 NM_033061.3 75.2% 70.3% (many diffs)
3 human 84616 KRTAP4-4 keratin associated protein 4-4 NM_032524.2 70.4% 69% (many diffs)
4 human 728224 KRTAP4-8 keratin associated protein 4-8 NM_031960.3 66% 57.8% (many diffs)
5 human 85289 KRTAP4-5 keratin associated protein 4-5 NM_033188.4 64.2% 59.6% (many diffs)
6 human 83755 KRTAP4-12 keratin associated protein ... NM_031854.3 64% 62.6% (many diffs)
7 human 653240 KRTAP4-11 keratin associated protein ... NM_033059.4 63.1% 56.9% (many diffs)
8 human 728224 KRTAP4-8 keratin associated protein 4-8 NM_001322457.1 62.6% 54.8% (many diffs)
9 human 81871 KRTAP4-6 keratin associated protein 4-6 NM_030976.1 60% 58.5% (many diffs)
10 human 100996750 LOC100996750 keratin-associated protein 4-7 NM_001365252.1 57.3% 52.8% (many diffs)
11 human 100132386 KRTAP4-9 keratin associated protein 4-9 NM_001146041.1 56.8% 53.8% (many diffs)
12 mouse 69464 Krtap4-13 keratin associated protein ... NM_027087.3 70.5% 66.2% (many diffs)
13 mouse 670482 Gm11554 predicted gene 11554 NM_001099313.2 62.5% 56.5% (many diffs)
14 mouse 670472 Gm11569 predicted gene 11569 NM_001099312.1 62% 55.3% (many diffs)
15 mouse 68768 Krtap4-6 keratin associated protein 4-6 NM_026834.2 61.4% 52% (many diffs)
16 mouse 100040248 Gm11563 predicted gene 11563 NM_001126320.2 60.9% 60.5% (many diffs)
17 mouse 670496 Gm11564 predicted gene 11564 NM_001100614.1 60.6% 53.4% (many diffs)
18 mouse 670464 Gm11596 predicted gene 11596 NM_001099311.2 59.2% 50% (many diffs)
19 mouse 76444 Krtap4-7 keratin associated protein 4-7 NM_029613.1 56.8% 53.2% (many diffs)
20 mouse 665992 Krtap4-8 keratin associated protein 4-8 NM_001085547.2 54% 52.3% (many diffs)
21 mouse 665998 Krtap4-9 keratin associated protein 4-9 NM_001085548.2 48.9% 44.4% (many diffs)
22 mouse 100040276 Gm11595 predicted gene 11595 NM_001126322.1 41.5% 37.5% (many diffs)
23 mouse 100502865 2300003K06Rik RIKEN cDNA 2300003K06 gene NM_001195383.1 40.6% 32.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
477
ORF length:
408
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGTCAACTCC TGTTGTGGCT CTGTGTGCTC TGACCAGGGC TGTGGCCTAG
121AGAACTGCTG CCGTCCCAGC TGCTGCCAGA CCACCTGCTG CAGGACCACC TGCTGCCGCC
181CCAGCTGCTG TGTGTCCAGC TGCTGCAGAC CGCAGTGCTG CCAGTCTGTG TGCTGCCAGC
241CCACCTGCTG CAGCCCCAGC TGCTGCCAGA CCACTTGCTG CAGGACCACC TGCTGCCGTC
301CCAGCTGCTG TGTGTCCAGC TGCTTCAGAC CCCAGTGCTG CCAGTCTGTG TGCTGCCAGC
361CCACCTGCTG CCGCCCCAGC TGTGGCCAGA CCACCTGCTG CAGGACCACC TGCTACCGCC
421CCAGCTGCTG TGTGTCCACC TGCTGCCGCC CAACCTGCTC TAGTGGCTCT TGCTGCTTGC
481CAACTTTCTT GTACAAAGTt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
541aac

Download FASTA (ORF) (Full)