Construct: ORF ccsbBroadEn_04487
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012181.1_s300c1, BRDN0000386986
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSFY1 (86614)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 86614 | HSFY1 | heat shock transcription fa... | NM_033108.2 | 100% | 100% | |
| 2 | human | 159119 | HSFY2 | heat shock transcription fa... | NM_153716.2 | 100% | 100% | |
| 3 | human | 159119 | HSFY2 | heat shock transcription fa... | XM_017030030.2 | 81.1% | 79.6% | (many diffs) |
| 4 | human | 86614 | HSFY1 | heat shock transcription fa... | XM_017030085.2 | 81.1% | 79.6% | (many diffs) |
| 5 | human | 159119 | HSFY2 | heat shock transcription fa... | XM_005262508.4 | 80.4% | 80% | 0_1ins217;4C>A;8_9insCTTTCTCTGAACTTTCC |
| 6 | human | 86614 | HSFY1 | heat shock transcription fa... | XM_005262565.4 | 80.4% | 80% | 0_1ins217;4C>A;8_9insCTTTCTCTGAACTTTCC |
| 7 | human | 27437 | HSFY1P1 | HSFY1 pseudogene 1 | NR_003607.1 | 73% | (many diffs) | |
| 8 | human | 159119 | HSFY2 | heat shock transcription fa... | XM_017030031.2 | 49.6% | 44.5% | (many diffs) |
| 9 | human | 159119 | HSFY2 | heat shock transcription fa... | NM_001001877.1 | 47.3% | 44.3% | (many diffs) |
| 10 | human | 86614 | HSFY1 | heat shock transcription fa... | NM_152584.1 | 47.3% | 44.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1269
- ORF length:
- 1203
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC ACATGTTTCT TCAGAAACTC AAGATGTTTC CCCCAAAGAT GAATTAACTG 121 CTTCAGAAGC CTCCACTAGG TCTCCATTGT GTGAACACAC CTTCCCTGGG GACTCAGACT 181 TACGGTCAAT GATTGAAGAA CATGCTTTTC AGGTTTTGTC ACAAGGATCC TTGTTAGAAA 241 GTCCAAGTTA CACAGTTTGT GTCTCTGAGC CAGATAAAGA TGATGATTTT CTTTCTCTGA 301 ACTTTCCCAG GAAACTTTGG AAAATAGTGG AAAGTGACCA ATTCAAGTCT ATTTCATGGG 361 ATGAGAATGG AACTTGCATA GTGATTAATG AAGAACTCTT CAAGAAAGAA ATTTTGGAAA 421 CAAAGGCTCC TTACAGAATA TTTCAAACTG ATGCTATCAA AAGTTTTGTT CGACAGCTCA 481 ACCTTTATGG ATTTAGTAAA ATTCAACAGA ATTTTCAAAG ATCTGCCTTT CTAGCCACCT 541 TTCTGTCAGA AGAGAAAGAA TCGTCTGTCT TAAGCAAGTT AAAGTTCTAT TATAATCCAA 601 ATTTCAAGCG TGGCTATCCC CAACTTTTAG TAAGAGTGAA GAGAAGAATT GGTGTTAAAA 661 ATGCTTCACC TATATCTACT TTATTCAACG AAGATTTCAA CAAGAAGCAT TTTAGAGCAG 721 GGGCTAACAT GGAGAATCAT AATTCTGCCT TAGCTGCTGA AGCTAGTGAA GAAAGTTTAT 781 TTTCAGCCTC TAAAAATTTA AATATGCCTC TAACAAGGGA ATCTTCTGTC AGACAGATAA 841 TTGCAAATTC ATCTGTCCCC ATTAGAAGTG GTTTCCCTCC TCCTTCACCT TCAACCTCAG 901 TTGGACCATC AGAACAAATT GCAACAGATC AACATGCTAT TTTAAATCAG TTGACCACTA 961 TTCATATGCA CTCTCATAGT ACCTACATGC AAGCAAGGGG CCACATTGTG AATTTTATTA 1021 CAACCACAAC TTCTCAATAC CACATCATAT CTCCCTTACA AAATGGTTAT TTTGGGCTGA 1081 CAGTGGAACC ATCTGCTGTT CCCACACGAT ATCCTCTGGT ATCAGTCAAT GAGGCTCCAT 1141 ATCGTAACAT GCTACCAGCA GGCAACCCGT GGTTGCAAAT GCCTACGATC GCTGATAGAT 1201 CAGCTGCCCC TCATTCCAGG CTAGCTCTTC AACCATCACC ACTGGACAAA TATCACCCTA 1261 ATTACAACTG CCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa 1321 tttgttgcaa cgaac