Construct: ORF ccsbBroadEn_04498
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014556.1_s300c1, BRDN0000387276
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BMERB1 (89927)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89927 | BMERB1 | bMERB domain containing 1 | NM_033201.3 | 100% | 100% | |
| 2 | human | 89927 | BMERB1 | bMERB domain containing 1 | NM_001142469.1 | 88.1% | 83.9% | (many diffs) |
| 3 | mouse | 67254 | Bmerb1 | bMERB domain containing 1 | NM_144518.3 | 91.1% | 95% | (many diffs) |
| 4 | mouse | 67254 | Bmerb1 | bMERB domain containing 1 | XM_006522482.4 | 61.7% | 61.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 678
- ORF length:
- 612
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGA ATTAAAGCAA TCTTTGTCCA CCCATCTGGA AGCCGAGAAG CCTCTGAGGC 121 GCTATGGGGC GGTGGAGGAG ACGGCTTGGA AAACGGAGAG ACTGGGGAGA AATCAGCTGG 181 ACATCATCTC CATGGCGGAG ACAACCATGA TGCCAGAGGA GATTGAGCTG GAGATGGCAA 241 AAATTCAGCG TCTCCGGGAA GTCTTGGTCC GCCGGGAGTC TGAGCTCAGG TTCATGATGG 301 ATGACATCCA GCTCTGCAAG GACATCATGG ACTTGAAGCA GGAGCTGCAG AACTTGGTCG 361 CCATCCCAGA AAAAGAAAAA ACCAAACTGC AGAAGCAGAG AGAGGATGAG CTAATCCAGA 421 AGATCCACAA ACTGGTGCAG AAGAGAGACT TCCTGGTGGA CGATGCGGAG GTCGAGCGGT 481 TAAGGGAGCA AGAAGAAGAC AAGGAAATGG CTGATTTCCT GAGAATCAAG TTAAAACCTC 541 TAGACAAAGT AACCAAATCT CCAGCCAGCT CCCGGGCAGA GAAGAAAGCA GAGCCCCCAC 601 CTAGCAAGCC CACGGTGGCC AAGACGGGGC TGGCACTGAT CAAGGATTGT TGCGGGGCCA 661 CCCAGTGCAA CATCATGTAC CCAACTTTCT TGTACAAAGT tggcattata agaaagcatt 721 gcttatcaat ttgttgcaac gaac